Three regulatory elements upstream of LMO4 are strongly associated with intermittent fertilization intensity in Chicken
Intermittent fertilization intensity (IFI) is closely related to higher fertilization in chicken hens. Recent studies have identified genes influencing sperm-oocyte interactions and immune regulation that impact the fertilization process in chickens. This research aims to identify key candidate gene...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125000069 |
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| author | Lei Wang Weijian Fan Xiuping Wang Yangming Pan Xing Hong Mingze Li ShiJun Li |
| author_facet | Lei Wang Weijian Fan Xiuping Wang Yangming Pan Xing Hong Mingze Li ShiJun Li |
| author_sort | Lei Wang |
| collection | DOAJ |
| description | Intermittent fertilization intensity (IFI) is closely related to higher fertilization in chicken hens. Recent studies have identified genes influencing sperm-oocyte interactions and immune regulation that impact the fertilization process in chickens. This research aims to identify key candidate genes and variants regulating IFI through transcriptomic analysis and dual luciferase assays. Our study's transcriptomic analysis of 12 individuals exhibiting extreme IFI revealed several key candidate genes. Validation using quantitative PCR highlighted PRSS12, DNER, WIF1, and NRXN1 as potential contributors to variations in IFI. Notably, we observed significant differences in the expression of LMO4, located 247.2 kb downstream of IFI-associated genomic regions. To explore variants potentially involved in the regulation of LMO4, we conducted short variant annotation and SV-GWAS, but found no significant associations with IFI. Further motif analysis and dual luciferase validation uncovered three regulatory elements within the associated region that exhibited enhanced promoter or enhancer activity following significant SNP mutations. In conclusion, our findings indicate that LMO4, PRSS12, DNER, WIF1, and NRXN1 serve as primary candidate genes for regulating IFI. Additionally, three regulatory elements significantly associated with IFI were identified upstream of LMO4. These variants hold promise for use in selecting low-IFI lines. |
| format | Article |
| id | doaj-art-00a2894c17bb46f680c346e31e0d02ca |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-00a2894c17bb46f680c346e31e0d02ca2025-02-08T04:59:17ZengElsevierPoultry Science0032-57912025-03-011043104769Three regulatory elements upstream of LMO4 are strongly associated with intermittent fertilization intensity in ChickenLei Wang0Weijian Fan1Xiuping Wang2Yangming Pan3Xing Hong4Mingze Li5ShiJun Li6Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei Province 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei Province 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei Province 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei Province 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei Province 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei Province 430070, ChinaKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China; Yazhouwan National Laboratory, Sanya 572024, PR China; Key Laboratory of Smart Farming for Agricultural Animals, Ministry of Education, Huazhong Agricultural University, Wuhan, Hubei Province 430070, China; Hubei Hongshan Laboratory, Wuhan, Hubei Province 430070, China; Corresponding author.Intermittent fertilization intensity (IFI) is closely related to higher fertilization in chicken hens. Recent studies have identified genes influencing sperm-oocyte interactions and immune regulation that impact the fertilization process in chickens. This research aims to identify key candidate genes and variants regulating IFI through transcriptomic analysis and dual luciferase assays. Our study's transcriptomic analysis of 12 individuals exhibiting extreme IFI revealed several key candidate genes. Validation using quantitative PCR highlighted PRSS12, DNER, WIF1, and NRXN1 as potential contributors to variations in IFI. Notably, we observed significant differences in the expression of LMO4, located 247.2 kb downstream of IFI-associated genomic regions. To explore variants potentially involved in the regulation of LMO4, we conducted short variant annotation and SV-GWAS, but found no significant associations with IFI. Further motif analysis and dual luciferase validation uncovered three regulatory elements within the associated region that exhibited enhanced promoter or enhancer activity following significant SNP mutations. In conclusion, our findings indicate that LMO4, PRSS12, DNER, WIF1, and NRXN1 serve as primary candidate genes for regulating IFI. Additionally, three regulatory elements significantly associated with IFI were identified upstream of LMO4. These variants hold promise for use in selecting low-IFI lines.http://www.sciencedirect.com/science/article/pii/S0032579125000069Reproduction traitsCandidate geneRegulatory variation |
| spellingShingle | Lei Wang Weijian Fan Xiuping Wang Yangming Pan Xing Hong Mingze Li ShiJun Li Three regulatory elements upstream of LMO4 are strongly associated with intermittent fertilization intensity in Chicken Poultry Science Reproduction traits Candidate gene Regulatory variation |
| title | Three regulatory elements upstream of LMO4 are strongly associated with intermittent fertilization intensity in Chicken |
| title_full | Three regulatory elements upstream of LMO4 are strongly associated with intermittent fertilization intensity in Chicken |
| title_fullStr | Three regulatory elements upstream of LMO4 are strongly associated with intermittent fertilization intensity in Chicken |
| title_full_unstemmed | Three regulatory elements upstream of LMO4 are strongly associated with intermittent fertilization intensity in Chicken |
| title_short | Three regulatory elements upstream of LMO4 are strongly associated with intermittent fertilization intensity in Chicken |
| title_sort | three regulatory elements upstream of lmo4 are strongly associated with intermittent fertilization intensity in chicken |
| topic | Reproduction traits Candidate gene Regulatory variation |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125000069 |
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