Cervical heterotopic heart transplantation in mice using a novel suture technique
Background: Vascularized transplantation models in mice are critical to understand mechanisms that mediate rejection and to develop new therapeutics. Standard abdominal heterotopic heart transplantation techniques employ an end-to-side suture technique and are the workhouse of transplant immunology...
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Elsevier
2025-02-01
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| Series: | JHLT Open |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2950133424001137 |
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| author | Hao Dun, MD MSc Maura Sticco-Ivins Yuriko Terada, MD PhD Amber Berning, MD Kory J. Lavine, MD PhD Daniel Kreisel, MD PhD Benjamin J. Kopecky, MD PhD |
| author_facet | Hao Dun, MD MSc Maura Sticco-Ivins Yuriko Terada, MD PhD Amber Berning, MD Kory J. Lavine, MD PhD Daniel Kreisel, MD PhD Benjamin J. Kopecky, MD PhD |
| author_sort | Hao Dun, MD MSc |
| collection | DOAJ |
| description | Background: Vascularized transplantation models in mice are critical to understand mechanisms that mediate rejection and to develop new therapeutics. Standard abdominal heterotopic heart transplantation techniques employ an end-to-side suture technique and are the workhouse of transplant immunology research laboratories. Recently, cervical heterotopic heart transplantation in mice has emerged as an alternative due to several advantages but is conventionally performed by suture or cuff techniques in an end-to-end fashion. Therefore, we introduce an end-to-side anastomosis technique. Methods: The donor pulmonary artery is end-to-side anastomosed to the recipient right external jugular vein, using a continuous 10–0 nylon suture. Vascular suturing is accomplished inside the vessel on the posterior wall, and then outside the vessel on the anterior wall. Finally, the donor ascending aorta is end-to-side anastomosed to the recipient common carotid artery with an identical suture technique. Results: The median times for the donor heart harvest, recipient preparation, anastomoses of the pulmonary artery to the external jugular vein, and the ascending aorta to the common carotid artery were 12, 10, 12 and 11 minutes, respectively. The survival rate was 100% (n = 20). Conclusions: We provide a detailed description of how to perform end-to-side anastomoses using a suture technique in the mouse cervical heart transplantation model. This procedure reconstitutes coronary blood flow in the heart graft with minimal interruption to recipient anatomy and provides an experimental platform to study transplant immunology. |
| format | Article |
| id | doaj-art-0708519b93344b6fb8d4c3eead68a0aa |
| institution | Kabale University |
| issn | 2950-1334 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | JHLT Open |
| spelling | doaj-art-0708519b93344b6fb8d4c3eead68a0aa2025-02-09T05:01:53ZengElsevierJHLT Open2950-13342025-02-017100164Cervical heterotopic heart transplantation in mice using a novel suture techniqueHao Dun, MD MSc0Maura Sticco-Ivins1Yuriko Terada, MD PhD2Amber Berning, MD3Kory J. Lavine, MD PhD4Daniel Kreisel, MD PhD5Benjamin J. Kopecky, MD PhD6Department of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus, Aurora, ColoradoDepartment of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus, Aurora, ColoradoDepartment of Surgery, Washington University, St. Louis, MissouriDepartment of Pathology, University of Colorado Anschutz Medical Campus, Aurora, ColoradoDepartment of Medicine, Division of Cardiology, Washington University, St. Louis, Missouri; Department of Pathology and Immunology, Washington University, St. Louis, Missouri; Department of Developmental Biology, Washington University, St. Louis, MissouriDepartment of Surgery, Washington University, St. Louis, Missouri; Department of Pathology and Immunology, Washington University, St. Louis, MissouriDepartment of Medicine, Division of Cardiology, University of Colorado Anschutz Medical Campus, Aurora, Colorado; Corresponding author: Benjamin J. Kopecky, MD, PhD, Department of Medicine, Division of Cardiology, University of Colorado, 12700 E 19th Ave, Mail Stop B139, Aurora, CO 80045-0508. Telephone: (303) 724-5471.Background: Vascularized transplantation models in mice are critical to understand mechanisms that mediate rejection and to develop new therapeutics. Standard abdominal heterotopic heart transplantation techniques employ an end-to-side suture technique and are the workhouse of transplant immunology research laboratories. Recently, cervical heterotopic heart transplantation in mice has emerged as an alternative due to several advantages but is conventionally performed by suture or cuff techniques in an end-to-end fashion. Therefore, we introduce an end-to-side anastomosis technique. Methods: The donor pulmonary artery is end-to-side anastomosed to the recipient right external jugular vein, using a continuous 10–0 nylon suture. Vascular suturing is accomplished inside the vessel on the posterior wall, and then outside the vessel on the anterior wall. Finally, the donor ascending aorta is end-to-side anastomosed to the recipient common carotid artery with an identical suture technique. Results: The median times for the donor heart harvest, recipient preparation, anastomoses of the pulmonary artery to the external jugular vein, and the ascending aorta to the common carotid artery were 12, 10, 12 and 11 minutes, respectively. The survival rate was 100% (n = 20). Conclusions: We provide a detailed description of how to perform end-to-side anastomoses using a suture technique in the mouse cervical heart transplantation model. This procedure reconstitutes coronary blood flow in the heart graft with minimal interruption to recipient anatomy and provides an experimental platform to study transplant immunology.http://www.sciencedirect.com/science/article/pii/S2950133424001137transplant immunologyheart transplantationend-to-side anastomosiscervical transplantationsuture technique |
| spellingShingle | Hao Dun, MD MSc Maura Sticco-Ivins Yuriko Terada, MD PhD Amber Berning, MD Kory J. Lavine, MD PhD Daniel Kreisel, MD PhD Benjamin J. Kopecky, MD PhD Cervical heterotopic heart transplantation in mice using a novel suture technique JHLT Open transplant immunology heart transplantation end-to-side anastomosis cervical transplantation suture technique |
| title | Cervical heterotopic heart transplantation in mice using a novel suture technique |
| title_full | Cervical heterotopic heart transplantation in mice using a novel suture technique |
| title_fullStr | Cervical heterotopic heart transplantation in mice using a novel suture technique |
| title_full_unstemmed | Cervical heterotopic heart transplantation in mice using a novel suture technique |
| title_short | Cervical heterotopic heart transplantation in mice using a novel suture technique |
| title_sort | cervical heterotopic heart transplantation in mice using a novel suture technique |
| topic | transplant immunology heart transplantation end-to-side anastomosis cervical transplantation suture technique |
| url | http://www.sciencedirect.com/science/article/pii/S2950133424001137 |
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