Reactivatable stimulated emission depletion microscopy using fluorescence-recoverable nanographene
Abstract Stimulated emission depletion (STED) microscopy, a key optical super-resolution imaging method, has extended our ability to view details to resolution levels of tens of nanometers. Its resolution depends on fluorophore de-excitation efficiency, and increases with depletion laser power. Howe...
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Nature Portfolio
2025-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-56401-z |
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| author | Qiqi Yang Antonio Virgilio Failla Petri Turunen Ana Mateos-Maroto Meiyu Gai Werner Zuschratter Sophia Westendorf Márton Gelléri Qiang Chen Goudappagouda Hao Zhao Xingfu Zhu Svenja Morsbach Marcus Scheele Wei Yan Katharina Landfester Ryota Kabe Mischa Bonn Akimitsu Narita Xiaomin Liu |
| author_facet | Qiqi Yang Antonio Virgilio Failla Petri Turunen Ana Mateos-Maroto Meiyu Gai Werner Zuschratter Sophia Westendorf Márton Gelléri Qiang Chen Goudappagouda Hao Zhao Xingfu Zhu Svenja Morsbach Marcus Scheele Wei Yan Katharina Landfester Ryota Kabe Mischa Bonn Akimitsu Narita Xiaomin Liu |
| author_sort | Qiqi Yang |
| collection | DOAJ |
| description | Abstract Stimulated emission depletion (STED) microscopy, a key optical super-resolution imaging method, has extended our ability to view details to resolution levels of tens of nanometers. Its resolution depends on fluorophore de-excitation efficiency, and increases with depletion laser power. However, high-power irradiation permanently turns off the fluorescence due to photo-bleaching of the fluorophores. As a result, there is a trade-off between spatial resolution and imaging time. Here, we overcome this limitation by introducing reactivatable STED (ReSTED) based on the photophysical properties of the nanographene dibenzo[hi,st]ovalene (DBOV). In contrast to the photo-induced decomposition of other fluorophores, the fluorescence of DBOV is only temporarily deactivated and can be reactivated by near-infrared light (including the 775 nm depletion beam). As a result, this fluorophore allows for hours-long, high-resolution 3D STED imaging, greatly expanding the applications of STED microscopy. |
| format | Article |
| id | doaj-art-07308c2015c84580b934b5624a9c3799 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-07308c2015c84580b934b5624a9c37992025-02-09T12:46:09ZengNature PortfolioNature Communications2041-17232025-02-0116111310.1038/s41467-025-56401-zReactivatable stimulated emission depletion microscopy using fluorescence-recoverable nanographeneQiqi Yang0Antonio Virgilio Failla1Petri Turunen2Ana Mateos-Maroto3Meiyu Gai4Werner Zuschratter5Sophia Westendorf6Márton Gelléri7Qiang Chen8Goudappagouda9Hao Zhao10Xingfu Zhu11Svenja Morsbach12Marcus Scheele13Wei Yan14Katharina Landfester15Ryota Kabe16Mischa Bonn17Akimitsu Narita18Xiaomin Liu19Max Planck Institute for Polymer ResearchUKE Microscopy Imaging Facility, University Medical Center Hamburg-EppendorfInstitute of Molecular Biology gGmbHMax Planck Institute for Polymer ResearchMax Planck Institute for Polymer ResearchLeibniz Institute for NeurobiologyInstitute of Physical and Theoretical Chemistry, University of TuebingenInstitute of Molecular Biology gGmbHMax Planck Institute for Polymer ResearchOrganic and Carbon Nanomaterials Unit, Okinawa Institute of Science and Technology Graduate UniversityOrganic and Carbon Nanomaterials Unit, Okinawa Institute of Science and Technology Graduate UniversityMax Planck Institute for Polymer ResearchMax Planck Institute for Polymer ResearchInstitute of Physical and Theoretical Chemistry, University of TuebingenKey Laboratory of 3D Micro/Nano Fabrication and Characterization of Zhejiang Province, School of Engineering, Westlake UniversityMax Planck Institute for Polymer ResearchOrganic Optoelectronics Unit, Okinawa Institute of Science and Technology Graduate UniversityMax Planck Institute for Polymer ResearchMax Planck Institute for Polymer ResearchMax Planck Institute for Polymer ResearchAbstract Stimulated emission depletion (STED) microscopy, a key optical super-resolution imaging method, has extended our ability to view details to resolution levels of tens of nanometers. Its resolution depends on fluorophore de-excitation efficiency, and increases with depletion laser power. However, high-power irradiation permanently turns off the fluorescence due to photo-bleaching of the fluorophores. As a result, there is a trade-off between spatial resolution and imaging time. Here, we overcome this limitation by introducing reactivatable STED (ReSTED) based on the photophysical properties of the nanographene dibenzo[hi,st]ovalene (DBOV). In contrast to the photo-induced decomposition of other fluorophores, the fluorescence of DBOV is only temporarily deactivated and can be reactivated by near-infrared light (including the 775 nm depletion beam). As a result, this fluorophore allows for hours-long, high-resolution 3D STED imaging, greatly expanding the applications of STED microscopy.https://doi.org/10.1038/s41467-025-56401-z |
| spellingShingle | Qiqi Yang Antonio Virgilio Failla Petri Turunen Ana Mateos-Maroto Meiyu Gai Werner Zuschratter Sophia Westendorf Márton Gelléri Qiang Chen Goudappagouda Hao Zhao Xingfu Zhu Svenja Morsbach Marcus Scheele Wei Yan Katharina Landfester Ryota Kabe Mischa Bonn Akimitsu Narita Xiaomin Liu Reactivatable stimulated emission depletion microscopy using fluorescence-recoverable nanographene Nature Communications |
| title | Reactivatable stimulated emission depletion microscopy using fluorescence-recoverable nanographene |
| title_full | Reactivatable stimulated emission depletion microscopy using fluorescence-recoverable nanographene |
| title_fullStr | Reactivatable stimulated emission depletion microscopy using fluorescence-recoverable nanographene |
| title_full_unstemmed | Reactivatable stimulated emission depletion microscopy using fluorescence-recoverable nanographene |
| title_short | Reactivatable stimulated emission depletion microscopy using fluorescence-recoverable nanographene |
| title_sort | reactivatable stimulated emission depletion microscopy using fluorescence recoverable nanographene |
| url | https://doi.org/10.1038/s41467-025-56401-z |
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