Comparison of characteristics and immune responses between paired human nasal and bronchial epithelial organoids
Abstract Background The nasal epithelium, as part of a continuous and integrated airway epithelium, provides a more accessible sample source than the bronchial epithelium. However, the similarities and differences in gene expression patterns and immune responses between these two sites have not been...
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BMC
2025-02-01
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| Series: | Cell & Bioscience |
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| Online Access: | https://doi.org/10.1186/s13578-024-01342-1 |
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| author | Lu Zhu Wenhao Yang Jiaxin Luo Danli Lu Yanan Hu Rui Zhang Yan Li Li Qiu Zelian Chen Lina Chen Hanmin Liu |
| author_facet | Lu Zhu Wenhao Yang Jiaxin Luo Danli Lu Yanan Hu Rui Zhang Yan Li Li Qiu Zelian Chen Lina Chen Hanmin Liu |
| author_sort | Lu Zhu |
| collection | DOAJ |
| description | Abstract Background The nasal epithelium, as part of a continuous and integrated airway epithelium, provides a more accessible sample source than the bronchial epithelium. However, the similarities and differences in gene expression patterns and immune responses between these two sites have not been extensively studied. Results Four lines of matched nasal and bronchial airway epithelial cells obtained from the four patients were embedded in Matrigel and cultured in thechemically defined medium to generate patient-derived nasal organoids (NO) and bronchial organoids (BO). Histologic examination of nasal organoid tissue revealed high similarity and a reduced ciliary beat frequency compared to bronchial organoid tissue. Whole exome sequencing revealed that over 99% of single nucleotides were shared between the NO and matched BO and there was a 95% overlap in their RNA transcriptomes. RNA sequencing analysis of differentially expressed genes indicated a significant reduction in the immune response in NO. RSV infection revealed more productive replication in NO, with a downregulated immune pathway identified by RNA sequencing analysis and upregulated levels of pro-inflammatory cytokines in culture supernatants in NO compared to BO. Conclusions NO and BO serve as robust in vitro models, faithfully recapitulating the biological characteristics of upper respiratory epithelial cells. The different regions of respiratory epithelial cells exhibit distinct immune responses, underscoring their complementary roles in exploring airway immune mechanisms and disease pathophysiology. |
| format | Article |
| id | doaj-art-09c0dbc431044829865c3416c3942c53 |
| institution | Kabale University |
| issn | 2045-3701 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell & Bioscience |
| spelling | doaj-art-09c0dbc431044829865c3416c3942c532025-02-09T12:56:20ZengBMCCell & Bioscience2045-37012025-02-0115111810.1186/s13578-024-01342-1Comparison of characteristics and immune responses between paired human nasal and bronchial epithelial organoidsLu Zhu0Wenhao Yang1Jiaxin Luo2Danli Lu3Yanan Hu4Rui Zhang5Yan Li6Li Qiu7Zelian Chen8Lina Chen9Hanmin Liu10Department of Pediatric Pulmonology and Immunology, West China Second University Hospital, Sichuan UniversityDepartment of Pediatric Pulmonology and Immunology, West China Second University Hospital, Sichuan UniversityDepartment of Pediatric Pulmonology and Immunology, West China Second University Hospital, Sichuan UniversityDepartment of Pediatric Pulmonology and Immunology, West China Second University Hospital, Sichuan UniversityDepartment of Pediatric Pulmonology and Immunology, West China Second University Hospital, Sichuan UniversityDepartment of Pediatric Pulmonology and Immunology, West China Second University Hospital, Sichuan UniversityDepartment of Pediatric Pulmonology and Immunology, West China Second University Hospital, Sichuan UniversityDepartment of Pediatric Pulmonology and Immunology, West China Second University Hospital, Sichuan UniversityDepartment of Pediatric Pulmonology and Immunology, West China Second University Hospital, Sichuan UniversityDepartment of Pediatric Pulmonology and Immunology, West China Second University Hospital, Sichuan UniversityDepartment of Pediatric Pulmonology and Immunology, West China Second University Hospital, Sichuan UniversityAbstract Background The nasal epithelium, as part of a continuous and integrated airway epithelium, provides a more accessible sample source than the bronchial epithelium. However, the similarities and differences in gene expression patterns and immune responses between these two sites have not been extensively studied. Results Four lines of matched nasal and bronchial airway epithelial cells obtained from the four patients were embedded in Matrigel and cultured in thechemically defined medium to generate patient-derived nasal organoids (NO) and bronchial organoids (BO). Histologic examination of nasal organoid tissue revealed high similarity and a reduced ciliary beat frequency compared to bronchial organoid tissue. Whole exome sequencing revealed that over 99% of single nucleotides were shared between the NO and matched BO and there was a 95% overlap in their RNA transcriptomes. RNA sequencing analysis of differentially expressed genes indicated a significant reduction in the immune response in NO. RSV infection revealed more productive replication in NO, with a downregulated immune pathway identified by RNA sequencing analysis and upregulated levels of pro-inflammatory cytokines in culture supernatants in NO compared to BO. Conclusions NO and BO serve as robust in vitro models, faithfully recapitulating the biological characteristics of upper respiratory epithelial cells. The different regions of respiratory epithelial cells exhibit distinct immune responses, underscoring their complementary roles in exploring airway immune mechanisms and disease pathophysiology.https://doi.org/10.1186/s13578-024-01342-1Respiratory epithelial cellsOrganoidsImmune responsesRespiratory syncytial virus |
| spellingShingle | Lu Zhu Wenhao Yang Jiaxin Luo Danli Lu Yanan Hu Rui Zhang Yan Li Li Qiu Zelian Chen Lina Chen Hanmin Liu Comparison of characteristics and immune responses between paired human nasal and bronchial epithelial organoids Cell & Bioscience Respiratory epithelial cells Organoids Immune responses Respiratory syncytial virus |
| title | Comparison of characteristics and immune responses between paired human nasal and bronchial epithelial organoids |
| title_full | Comparison of characteristics and immune responses between paired human nasal and bronchial epithelial organoids |
| title_fullStr | Comparison of characteristics and immune responses between paired human nasal and bronchial epithelial organoids |
| title_full_unstemmed | Comparison of characteristics and immune responses between paired human nasal and bronchial epithelial organoids |
| title_short | Comparison of characteristics and immune responses between paired human nasal and bronchial epithelial organoids |
| title_sort | comparison of characteristics and immune responses between paired human nasal and bronchial epithelial organoids |
| topic | Respiratory epithelial cells Organoids Immune responses Respiratory syncytial virus |
| url | https://doi.org/10.1186/s13578-024-01342-1 |
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