The effect of Garcinia livingstonei aqueous leaf extract on hyperglycaemic-induced human sperm cell: An in-vitro study
Hyperglycaemia is the main instigator of the development of male infertility in men with diabetes mellitus (DM). The consequent production of excessive reactive oxidative species (ROS) and a compromised antioxidant defence system leads to testicular damage and a decline in sperm parameters. Suppleme...
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Elsevier
2025-02-01
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| Series: | Phytomedicine Plus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031325000235 |
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| author | Murendeni Nethengwe Kunle Okaiyeto Chinyerum S. Opuwari Oluwafemi O. Oguntibeju |
| author_facet | Murendeni Nethengwe Kunle Okaiyeto Chinyerum S. Opuwari Oluwafemi O. Oguntibeju |
| author_sort | Murendeni Nethengwe |
| collection | DOAJ |
| description | Hyperglycaemia is the main instigator of the development of male infertility in men with diabetes mellitus (DM). The consequent production of excessive reactive oxidative species (ROS) and a compromised antioxidant defence system leads to testicular damage and a decline in sperm parameters. Supplementation with antioxidants sourced from medicinal plants is beneficial in the treatment of DM-related male infertility. However, a search for more efficacious and easily accessibly medicinal plants is paramount. Garcinia livingstonei is a well-known and accessible medicinal plant used in traditional practices for the treatment of diabetic complications. However, very scarce information on the plant extract's potential benefits exists in the literature. The current study investigated the effect of aqueous leaf extract of G. livingstonei on hyperglycaemic human male sperm cell parameters. Hyperglycaemia was induced using sperm media supplemented with 100 mM D-glucose over 24 h. Hyperglycaemia induction led to a significant (P<0.05) decline in sperm motility, vitality, DNA integrity, mitochondrial membrane potential (MMP), and acrosome integrity. The effect of the plant extract on spermatozoa was concentration-dependent, with the lowest concentration demonstrating the highest protective effects. A significant (P<0.05) improvement in all tested sperm parameters was observed after exposure of the hyperglycaemic spermatozoa to aqueous leaf extract of G. livingstonei. The effect of acarbose on all tested sperm parameters was comparable to that of the plant extract. The findings of this study suggest the potential therapeutic effect of G. livingstonei in the treatment of DM-related male infertility. However, the use of an appropriate dosage depending on the pathological target is paramount. |
| format | Article |
| id | doaj-art-09fdea2dd1734f24ba1cc1b377dabd78 |
| institution | Kabale University |
| issn | 2667-0313 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj-art-09fdea2dd1734f24ba1cc1b377dabd782025-02-10T04:35:24ZengElsevierPhytomedicine Plus2667-03132025-02-0151100750The effect of Garcinia livingstonei aqueous leaf extract on hyperglycaemic-induced human sperm cell: An in-vitro studyMurendeni Nethengwe0Kunle Okaiyeto1Chinyerum S. Opuwari2Oluwafemi O. Oguntibeju3Phytomedicine and Phytochemistry Group, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Bellville 7535, South AfricaPhytomedicine and Phytochemistry Group, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Bellville 7535, South AfricaDepartment of Medical Biosciences, University of the Western Cape, Bellville, South AfricaPhytomedicine and Phytochemistry Group, Department of Biomedical Sciences, Faculty of Health and Wellness Sciences, Cape Peninsula University of Technology, Bellville 7535, South Africa; Corresponding author.Hyperglycaemia is the main instigator of the development of male infertility in men with diabetes mellitus (DM). The consequent production of excessive reactive oxidative species (ROS) and a compromised antioxidant defence system leads to testicular damage and a decline in sperm parameters. Supplementation with antioxidants sourced from medicinal plants is beneficial in the treatment of DM-related male infertility. However, a search for more efficacious and easily accessibly medicinal plants is paramount. Garcinia livingstonei is a well-known and accessible medicinal plant used in traditional practices for the treatment of diabetic complications. However, very scarce information on the plant extract's potential benefits exists in the literature. The current study investigated the effect of aqueous leaf extract of G. livingstonei on hyperglycaemic human male sperm cell parameters. Hyperglycaemia was induced using sperm media supplemented with 100 mM D-glucose over 24 h. Hyperglycaemia induction led to a significant (P<0.05) decline in sperm motility, vitality, DNA integrity, mitochondrial membrane potential (MMP), and acrosome integrity. The effect of the plant extract on spermatozoa was concentration-dependent, with the lowest concentration demonstrating the highest protective effects. A significant (P<0.05) improvement in all tested sperm parameters was observed after exposure of the hyperglycaemic spermatozoa to aqueous leaf extract of G. livingstonei. The effect of acarbose on all tested sperm parameters was comparable to that of the plant extract. The findings of this study suggest the potential therapeutic effect of G. livingstonei in the treatment of DM-related male infertility. However, the use of an appropriate dosage depending on the pathological target is paramount.http://www.sciencedirect.com/science/article/pii/S2667031325000235Garcinia livingstoneiHyperglycaemiaMale infertilityMedicinal plantsOxidative stress |
| spellingShingle | Murendeni Nethengwe Kunle Okaiyeto Chinyerum S. Opuwari Oluwafemi O. Oguntibeju The effect of Garcinia livingstonei aqueous leaf extract on hyperglycaemic-induced human sperm cell: An in-vitro study Phytomedicine Plus Garcinia livingstonei Hyperglycaemia Male infertility Medicinal plants Oxidative stress |
| title | The effect of Garcinia livingstonei aqueous leaf extract on hyperglycaemic-induced human sperm cell: An in-vitro study |
| title_full | The effect of Garcinia livingstonei aqueous leaf extract on hyperglycaemic-induced human sperm cell: An in-vitro study |
| title_fullStr | The effect of Garcinia livingstonei aqueous leaf extract on hyperglycaemic-induced human sperm cell: An in-vitro study |
| title_full_unstemmed | The effect of Garcinia livingstonei aqueous leaf extract on hyperglycaemic-induced human sperm cell: An in-vitro study |
| title_short | The effect of Garcinia livingstonei aqueous leaf extract on hyperglycaemic-induced human sperm cell: An in-vitro study |
| title_sort | effect of garcinia livingstonei aqueous leaf extract on hyperglycaemic induced human sperm cell an in vitro study |
| topic | Garcinia livingstonei Hyperglycaemia Male infertility Medicinal plants Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S2667031325000235 |
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