Effects of orally exposed SiO2 nanoparticles on lipid profiles in gut-liver axis of mice
Recently we proposed the possibility of orally exposed nanoparticles (NPs) to alter metabolite homeostasis by changing metabolism pathways, in addition to intestinal damages, but relatively few studies investigated the changes of metabolite profiles in multi-organs. This study investigated the influ...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324016567 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823856878280507392 |
|---|---|
| author | Kuanhang Li Zhengzheng Zhou Yi Cao |
| author_facet | Kuanhang Li Zhengzheng Zhou Yi Cao |
| author_sort | Kuanhang Li |
| collection | DOAJ |
| description | Recently we proposed the possibility of orally exposed nanoparticles (NPs) to alter metabolite homeostasis by changing metabolism pathways, in addition to intestinal damages, but relatively few studies investigated the changes of metabolite profiles in multi-organs. This study investigated the influences of orally exposed SiO2 NPs on lipid profiles in gut-liver axis. To this end, we treated mice with 16, 160 or 1600 mg/kg bodyweight SiO2 NPs via intragastric route. After 5 days exposure (once a day), we observed that SiO2 NPs induced minimal pathological changes but increased most of the trace elements. Furthermore, lipid staining was gradually decreased in intestines and livers with the increase of NP levels. Consistently, lipidomics results showed that most of the lipid classes in mouse intestines and livers were decreased following SiO2 NP administration. We further identified the lipid classes significantly decreased in both intestines and livers, such as phosphatidylserine (PS), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE). Only a few lipid classes, such as anandamide, showed opposite trends in these organs. For metabolism pathway, SiO2 NPs suppressed autophagy, showing as a significant decrease of microtubule-associated protein 1 A/1B light chain 3 (LC3) and adipose triglyceride lipase (Atgl), accompanying with an accumulation of P62, in both intestines and livers. However, lysosomal-associated membrane protein 2 (Lamp2) showed different trend, that it was significantly increased in intestines but decreased in livers. Combined, our results indicated that intragastric administration of SiO2 NPs altered trace element balance and lipid profiles, accompanying with a change of autophagic lipolysis proteins, in mouse gut-liver axis. |
| format | Article |
| id | doaj-art-0b560aa9a0494b8aa1e1398d739965c4 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-0b560aa9a0494b8aa1e1398d739965c42025-02-12T05:29:53ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117580Effects of orally exposed SiO2 nanoparticles on lipid profiles in gut-liver axis of miceKuanhang Li0Zhengzheng Zhou1Yi Cao2Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, ChinaNMPA Key Laboratory for Safety Evaluation of Cosmetics, Department of Hygiene Inspection & Quarantine Science, Guangdong Provincial Key Laboratory of Tropical Disease Research, School of Public Health, Southern Medical University, Guangzhou, Guangdong 510515, China; Corresponding authors.Hunan Province Key Laboratory of Typical Environmental Pollution and Health Hazards, School of Public Health, Hengyang Medical School, University of South China, Hengyang 421001, China; Corresponding authors.Recently we proposed the possibility of orally exposed nanoparticles (NPs) to alter metabolite homeostasis by changing metabolism pathways, in addition to intestinal damages, but relatively few studies investigated the changes of metabolite profiles in multi-organs. This study investigated the influences of orally exposed SiO2 NPs on lipid profiles in gut-liver axis. To this end, we treated mice with 16, 160 or 1600 mg/kg bodyweight SiO2 NPs via intragastric route. After 5 days exposure (once a day), we observed that SiO2 NPs induced minimal pathological changes but increased most of the trace elements. Furthermore, lipid staining was gradually decreased in intestines and livers with the increase of NP levels. Consistently, lipidomics results showed that most of the lipid classes in mouse intestines and livers were decreased following SiO2 NP administration. We further identified the lipid classes significantly decreased in both intestines and livers, such as phosphatidylserine (PS), phosphatidylglycerol (PG), and phosphatidylethanolamine (PE). Only a few lipid classes, such as anandamide, showed opposite trends in these organs. For metabolism pathway, SiO2 NPs suppressed autophagy, showing as a significant decrease of microtubule-associated protein 1 A/1B light chain 3 (LC3) and adipose triglyceride lipase (Atgl), accompanying with an accumulation of P62, in both intestines and livers. However, lysosomal-associated membrane protein 2 (Lamp2) showed different trend, that it was significantly increased in intestines but decreased in livers. Combined, our results indicated that intragastric administration of SiO2 NPs altered trace element balance and lipid profiles, accompanying with a change of autophagic lipolysis proteins, in mouse gut-liver axis.http://www.sciencedirect.com/science/article/pii/S0147651324016567SiO2 nanoparticles (NPs)Lipid profilesOral toxicityAutophagyTrace element balance |
| spellingShingle | Kuanhang Li Zhengzheng Zhou Yi Cao Effects of orally exposed SiO2 nanoparticles on lipid profiles in gut-liver axis of mice Ecotoxicology and Environmental Safety SiO2 nanoparticles (NPs) Lipid profiles Oral toxicity Autophagy Trace element balance |
| title | Effects of orally exposed SiO2 nanoparticles on lipid profiles in gut-liver axis of mice |
| title_full | Effects of orally exposed SiO2 nanoparticles on lipid profiles in gut-liver axis of mice |
| title_fullStr | Effects of orally exposed SiO2 nanoparticles on lipid profiles in gut-liver axis of mice |
| title_full_unstemmed | Effects of orally exposed SiO2 nanoparticles on lipid profiles in gut-liver axis of mice |
| title_short | Effects of orally exposed SiO2 nanoparticles on lipid profiles in gut-liver axis of mice |
| title_sort | effects of orally exposed sio2 nanoparticles on lipid profiles in gut liver axis of mice |
| topic | SiO2 nanoparticles (NPs) Lipid profiles Oral toxicity Autophagy Trace element balance |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324016567 |
| work_keys_str_mv | AT kuanhangli effectsoforallyexposedsio2nanoparticlesonlipidprofilesingutliveraxisofmice AT zhengzhengzhou effectsoforallyexposedsio2nanoparticlesonlipidprofilesingutliveraxisofmice AT yicao effectsoforallyexposedsio2nanoparticlesonlipidprofilesingutliveraxisofmice |