An Open-source Python Tool for Traction Force Microscopy on Micropatterned Substrates
Cell-generated forces play a critical role in driving and regulating complex biological processes, such as cell migration and division and cell and tissue morphogenesis in development and disease. Traction force microscopy (TFM) is an established technique developed in the field of mechanobiology us...
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| Language: | English |
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Bio-protocol LLC
2025-01-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5156&type=0 |
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| author | Artur Ruppel Vladimir Misiak Martial Balland |
| author_facet | Artur Ruppel Vladimir Misiak Martial Balland |
| author_sort | Artur Ruppel |
| collection | DOAJ |
| description | Cell-generated forces play a critical role in driving and regulating complex biological processes, such as cell migration and division and cell and tissue morphogenesis in development and disease. Traction force microscopy (TFM) is an established technique developed in the field of mechanobiology used to quantify cellular forces exerted on soft substrates and internal mechanical tissue stresses. TFM measures cell-generated traction forces in 2D or 3D environments with varying mechanical and biochemical properties. This technique involves embedding fiducial markers in the substrate, imaging substrate deformations caused by the cells, and using mathematical models to infer forces. This protocol compiles procedures from various previously published studies and software packages and describes how to perform TFM on 2D micropatterned substrates. Although not the focus of this protocol, the methods and software packages shown here also allow to perform monolayer stress microscopy (MSM), a method to calculate internal mechanical stress within the cells by modeling them as a thin plate with linear and homogeneous material properties. TFM and MSM are non-invasive methods capable of yielding spatially and temporally resolved force and stress maps with high throughput. As such, they enable the generation of rich datasets, which can provide valuable insights into the roles of cell-generated forces in various physiological and pathological processes. |
| format | Article |
| id | doaj-art-0c3869b414b243c4ac4ee7aa4b8ac066 |
| institution | Kabale University |
| issn | 2331-8325 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-0c3869b414b243c4ac4ee7aa4b8ac0662025-02-07T08:16:31ZengBio-protocol LLCBio-Protocol2331-83252025-01-0115110.21769/BioProtoc.5156An Open-source Python Tool for Traction Force Microscopy on Micropatterned SubstratesArtur Ruppel0Vladimir Misiak1Martial Balland2Centre de Recherche en Biologie cellulaire de Montpellier (CRBM), Université de Montpellier, CNRS, Montpellier, FranceLaboratoire Interdisciplinaire de Physique (LIPhy), Université Grenoble Alpes, CNRS, Grenoble, FranceLaboratoire Interdisciplinaire de Physique (LIPhy), Université Grenoble Alpes, CNRS, Grenoble, FranceCell-generated forces play a critical role in driving and regulating complex biological processes, such as cell migration and division and cell and tissue morphogenesis in development and disease. Traction force microscopy (TFM) is an established technique developed in the field of mechanobiology used to quantify cellular forces exerted on soft substrates and internal mechanical tissue stresses. TFM measures cell-generated traction forces in 2D or 3D environments with varying mechanical and biochemical properties. This technique involves embedding fiducial markers in the substrate, imaging substrate deformations caused by the cells, and using mathematical models to infer forces. This protocol compiles procedures from various previously published studies and software packages and describes how to perform TFM on 2D micropatterned substrates. Although not the focus of this protocol, the methods and software packages shown here also allow to perform monolayer stress microscopy (MSM), a method to calculate internal mechanical stress within the cells by modeling them as a thin plate with linear and homogeneous material properties. TFM and MSM are non-invasive methods capable of yielding spatially and temporally resolved force and stress maps with high throughput. As such, they enable the generation of rich datasets, which can provide valuable insights into the roles of cell-generated forces in various physiological and pathological processes.https://bio-protocol.org/en/bpdetail?id=5156&type=0 |
| spellingShingle | Artur Ruppel Vladimir Misiak Martial Balland An Open-source Python Tool for Traction Force Microscopy on Micropatterned Substrates Bio-Protocol |
| title | An Open-source Python Tool for Traction Force Microscopy on Micropatterned Substrates |
| title_full | An Open-source Python Tool for Traction Force Microscopy on Micropatterned Substrates |
| title_fullStr | An Open-source Python Tool for Traction Force Microscopy on Micropatterned Substrates |
| title_full_unstemmed | An Open-source Python Tool for Traction Force Microscopy on Micropatterned Substrates |
| title_short | An Open-source Python Tool for Traction Force Microscopy on Micropatterned Substrates |
| title_sort | open source python tool for traction force microscopy on micropatterned substrates |
| url | https://bio-protocol.org/en/bpdetail?id=5156&type=0 |
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