Exploring the efficacy of AMACR, ERG, and AR immunostains in prostatic adenocarcinoma and their association with novel grade groups
The study examines the utility of AMACR, ERG, and AR immunostains in diagnosing prostatic adenocarcinoma (PCa) and assessing prognosis in comparison to the Gleason score and new WHO grading groups. Seventeen PCa biopsies and five benign prostatic hyperplasia (BPH) biopsies were analyzed. Immunoreact...
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| Format: | Article |
| Language: | English |
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PAGEPress Publications
2025-02-01
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| Series: | European Journal of Histochemistry |
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| Online Access: | https://www.ejh.it/index.php/ejh/article/view/4172 |
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| author | Mohamed O. Andarawi Hassan Otifi Hesham Hassan Adil A. Yousif Saadalnour A. Mustafa Shawgi A. Elsiddig Asad Ma Babker Elryah I. Ali Omer Osman Elhag |
| author_facet | Mohamed O. Andarawi Hassan Otifi Hesham Hassan Adil A. Yousif Saadalnour A. Mustafa Shawgi A. Elsiddig Asad Ma Babker Elryah I. Ali Omer Osman Elhag |
| author_sort | Mohamed O. Andarawi |
| collection | DOAJ |
| description | The study examines the utility of AMACR, ERG, and AR immunostains in diagnosing prostatic adenocarcinoma (PCa) and assessing prognosis in comparison to the Gleason score and new WHO grading groups. Seventeen PCa biopsies and five benign prostatic hyperplasia (BPH) biopsies were analyzed. Immunoreactivity, scored from 1 to 3 based on percentage of positive cells and intensity of expression, was assessed, revealing 76.47% positivity for AMACR, 35.29% for ERG, and 94.12% for AR in PCa cases, with variable scores and intensity among markers and grade groups. AMACR sensitivity and ERG specificity were noted. Higher-grade PCa exhibited increased positivity for both markers, indicating prognostic significance. In BPH cases, AMACR showed positivity in 2 cases, ERG in 1, and AR in all cases, albeit with lower expression. Differential expression was observed among immunomarkers and grade groups of malignancy. AMACR and ERG stains serve as sensitive and specific markers for PCa diagnosis and prognosis. Their increasing positivity with higher-grade groups underscores prognostic value. These findings highlight the importance of immunostains in refining PCa diagnosis and prognostication.
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| format | Article |
| id | doaj-art-0d594e9749244418acafd346b7d06514 |
| institution | Kabale University |
| issn | 1121-760X 2038-8306 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj-art-0d594e9749244418acafd346b7d065142025-02-12T07:55:01ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062025-02-0169110.4081/ejh.2025.4172Exploring the efficacy of AMACR, ERG, and AR immunostains in prostatic adenocarcinoma and their association with novel grade groupsMohamed O. Andarawi0https://orcid.org/0000-0002-9027-8449Hassan Otifi1Hesham Hassan2Adil A. Yousif 3https://orcid.org/0009-0004-2438-0367Saadalnour A. Mustafa4Shawgi A. Elsiddig 5https://orcid.org/0000-0001-8210-4361Asad Ma Babker6https://orcid.org/0000-0002-5022-5676Elryah I. Ali 7https://orcid.org/0000-0002-7444-0944Omer Osman Elhag8Pathology Department, College of Medicine, King Khalid University, AbhaPathology Department, College of Medicine, King Khalid University, AbhaPathology Department, College of Medicine, King Khalid University, AbhaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, AbhaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Najran UniversityClinical Laboratory Sciences Department, College of Applied Medical Sciences, Jouf University, SakakaDepartment of Medical Laboratory Sciences, College of Health Sciences, Gulf Medical University, AjmanDepartment of Medical Laboratory Technology, College of Applied Medical Sciences, Northern Border University, ArarDepartment of Histopathology and Cytology, Faculty of Medical Laboratory Sciences, Omdurman Islamic UniversityThe study examines the utility of AMACR, ERG, and AR immunostains in diagnosing prostatic adenocarcinoma (PCa) and assessing prognosis in comparison to the Gleason score and new WHO grading groups. Seventeen PCa biopsies and five benign prostatic hyperplasia (BPH) biopsies were analyzed. Immunoreactivity, scored from 1 to 3 based on percentage of positive cells and intensity of expression, was assessed, revealing 76.47% positivity for AMACR, 35.29% for ERG, and 94.12% for AR in PCa cases, with variable scores and intensity among markers and grade groups. AMACR sensitivity and ERG specificity were noted. Higher-grade PCa exhibited increased positivity for both markers, indicating prognostic significance. In BPH cases, AMACR showed positivity in 2 cases, ERG in 1, and AR in all cases, albeit with lower expression. Differential expression was observed among immunomarkers and grade groups of malignancy. AMACR and ERG stains serve as sensitive and specific markers for PCa diagnosis and prognosis. Their increasing positivity with higher-grade groups underscores prognostic value. These findings highlight the importance of immunostains in refining PCa diagnosis and prognostication. https://www.ejh.it/index.php/ejh/article/view/4172Prostatic adenocarcinomaGleason scoreAMACRERGARPSA |
| spellingShingle | Mohamed O. Andarawi Hassan Otifi Hesham Hassan Adil A. Yousif Saadalnour A. Mustafa Shawgi A. Elsiddig Asad Ma Babker Elryah I. Ali Omer Osman Elhag Exploring the efficacy of AMACR, ERG, and AR immunostains in prostatic adenocarcinoma and their association with novel grade groups European Journal of Histochemistry Prostatic adenocarcinoma Gleason score AMACR ERG AR PSA |
| title | Exploring the efficacy of AMACR, ERG, and AR immunostains in prostatic adenocarcinoma and their association with novel grade groups |
| title_full | Exploring the efficacy of AMACR, ERG, and AR immunostains in prostatic adenocarcinoma and their association with novel grade groups |
| title_fullStr | Exploring the efficacy of AMACR, ERG, and AR immunostains in prostatic adenocarcinoma and their association with novel grade groups |
| title_full_unstemmed | Exploring the efficacy of AMACR, ERG, and AR immunostains in prostatic adenocarcinoma and their association with novel grade groups |
| title_short | Exploring the efficacy of AMACR, ERG, and AR immunostains in prostatic adenocarcinoma and their association with novel grade groups |
| title_sort | exploring the efficacy of amacr erg and ar immunostains in prostatic adenocarcinoma and their association with novel grade groups |
| topic | Prostatic adenocarcinoma Gleason score AMACR ERG AR PSA |
| url | https://www.ejh.it/index.php/ejh/article/view/4172 |
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