Fragrant olive (Osmanthus fragrans Lour.) flower extract attenuates dexamethasone-induced muscle atrophy through stimulation of PI3K/Akt pathway in C2C12 myotubes and C57BL/6 mice
Maintaining protein homeostasis is crucial to prevent muscle wasting caused by an imbalance between muscle protein synthesis and degradation. In this study, in vitro and in vivo experiments were performed using C2C12 myotubes and C57BL/6 mice treated with dexamethasone to investigate the anti-atroph...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464625000362 |
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| author | Taeuk Kim Seungok Lee Sein Lee Hyejung Han Chul-Hong Kim Dong-Woo Lee Jae-Kwan Hwang |
| author_facet | Taeuk Kim Seungok Lee Sein Lee Hyejung Han Chul-Hong Kim Dong-Woo Lee Jae-Kwan Hwang |
| author_sort | Taeuk Kim |
| collection | DOAJ |
| description | Maintaining protein homeostasis is crucial to prevent muscle wasting caused by an imbalance between muscle protein synthesis and degradation. In this study, in vitro and in vivo experiments were performed using C2C12 myotubes and C57BL/6 mice treated with dexamethasone to investigate the anti-atrophic effects of Osmanthus fragrans water extract (OFWE). In C2C12 myotubes, OFWE improved the protein synthesis by upregulating the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin pathway and muscle differentiation by activating myogenesis-related genes. Moreover, OFWE treatment reduced muscle protein degradation by regulating the nuclear translocation of forkhead box O3a and the expression of E3 ubiquitin ligases. In an animal model, OFWE administration was shown to increase muscle mass, myofiber size, and exercise capacity. OFWE also activated the PI3K/Akt pathway and inhibited protein catabolism. In conclusion, OFWE exhibited beneficial effects on muscle atrophy both in vitro and in vivo. |
| format | Article |
| id | doaj-art-0da5434f7a1145f2b35dd0a4eb4db645 |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-0da5434f7a1145f2b35dd0a4eb4db6452025-02-09T04:59:59ZengElsevierJournal of Functional Foods1756-46462025-02-01125106694Fragrant olive (Osmanthus fragrans Lour.) flower extract attenuates dexamethasone-induced muscle atrophy through stimulation of PI3K/Akt pathway in C2C12 myotubes and C57BL/6 miceTaeuk Kim0Seungok Lee1Sein Lee2Hyejung Han3Chul-Hong Kim4Dong-Woo Lee5Jae-Kwan Hwang6Graduate Program in Bioindustrial Engineering, Yonsei University, Seoul 03772, Republic of KoreaGraduate Program in Bioindustrial Engineering, Yonsei University, Seoul 03772, Republic of KoreaFood Research Center, Binggrae Co., Ltd., Namyangju 12253, Republic of KoreaFood Research Center, Binggrae Co., Ltd., Namyangju 12253, Republic of KoreaFood Research Center, Binggrae Co., Ltd., Namyangju 12253, Republic of KoreaGraduate Program in Bioindustrial Engineering, Yonsei University, Seoul 03772, Republic of Korea; Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea; Corresponding author at: Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea.Graduate Program in Bioindustrial Engineering, Yonsei University, Seoul 03772, Republic of Korea; Department of Biotechnology, Yonsei University, Seoul 03722, Republic of Korea; Corresponding author at: Graduate Program in Bioindustrial Engineering, Yonsei University, Seoul 03772, Republic of Korea.Maintaining protein homeostasis is crucial to prevent muscle wasting caused by an imbalance between muscle protein synthesis and degradation. In this study, in vitro and in vivo experiments were performed using C2C12 myotubes and C57BL/6 mice treated with dexamethasone to investigate the anti-atrophic effects of Osmanthus fragrans water extract (OFWE). In C2C12 myotubes, OFWE improved the protein synthesis by upregulating the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin pathway and muscle differentiation by activating myogenesis-related genes. Moreover, OFWE treatment reduced muscle protein degradation by regulating the nuclear translocation of forkhead box O3a and the expression of E3 ubiquitin ligases. In an animal model, OFWE administration was shown to increase muscle mass, myofiber size, and exercise capacity. OFWE also activated the PI3K/Akt pathway and inhibited protein catabolism. In conclusion, OFWE exhibited beneficial effects on muscle atrophy both in vitro and in vivo.http://www.sciencedirect.com/science/article/pii/S1756464625000362Osmanthus fragransFragrant oliveDexamethasoneMuscle atrophyPI3K/Akt |
| spellingShingle | Taeuk Kim Seungok Lee Sein Lee Hyejung Han Chul-Hong Kim Dong-Woo Lee Jae-Kwan Hwang Fragrant olive (Osmanthus fragrans Lour.) flower extract attenuates dexamethasone-induced muscle atrophy through stimulation of PI3K/Akt pathway in C2C12 myotubes and C57BL/6 mice Journal of Functional Foods Osmanthus fragrans Fragrant olive Dexamethasone Muscle atrophy PI3K/Akt |
| title | Fragrant olive (Osmanthus fragrans Lour.) flower extract attenuates dexamethasone-induced muscle atrophy through stimulation of PI3K/Akt pathway in C2C12 myotubes and C57BL/6 mice |
| title_full | Fragrant olive (Osmanthus fragrans Lour.) flower extract attenuates dexamethasone-induced muscle atrophy through stimulation of PI3K/Akt pathway in C2C12 myotubes and C57BL/6 mice |
| title_fullStr | Fragrant olive (Osmanthus fragrans Lour.) flower extract attenuates dexamethasone-induced muscle atrophy through stimulation of PI3K/Akt pathway in C2C12 myotubes and C57BL/6 mice |
| title_full_unstemmed | Fragrant olive (Osmanthus fragrans Lour.) flower extract attenuates dexamethasone-induced muscle atrophy through stimulation of PI3K/Akt pathway in C2C12 myotubes and C57BL/6 mice |
| title_short | Fragrant olive (Osmanthus fragrans Lour.) flower extract attenuates dexamethasone-induced muscle atrophy through stimulation of PI3K/Akt pathway in C2C12 myotubes and C57BL/6 mice |
| title_sort | fragrant olive osmanthus fragrans lour flower extract attenuates dexamethasone induced muscle atrophy through stimulation of pi3k akt pathway in c2c12 myotubes and c57bl 6 mice |
| topic | Osmanthus fragrans Fragrant olive Dexamethasone Muscle atrophy PI3K/Akt |
| url | http://www.sciencedirect.com/science/article/pii/S1756464625000362 |
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