Prevalence and molecular characterization of Beta-lactamase resistance gene in multidrug resistance bacteria, Proteus spp.
Existing of drug resistance bacteria in meat is a series of health concern and beta-lactamase is responsible to generate multi drug resistances in bacteria. Meat is a source of delivering food born pathogen bacteria including Proteus species. Recently Proteus bacteria developed drug resistance again...
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| Format: | Article |
| Language: | English |
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Sulaimani Polytechnic University
2019-08-01
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| Series: | Kurdistan Journal of Applied Research |
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| Online Access: | https://kjar.spu.edu.iq/index.php/kjar/article/view/307 |
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| author | Sabiha S. Salih Shno J. Mohammed Imad M Noori Lana MA Mohammed Taib A. Hama Soor |
| author_facet | Sabiha S. Salih Shno J. Mohammed Imad M Noori Lana MA Mohammed Taib A. Hama Soor |
| author_sort | Sabiha S. Salih |
| collection | DOAJ |
| description | Existing of drug resistance bacteria in meat is a series of health concern and beta-lactamase is responsible to generate multi drug resistances in bacteria. Meat is a source of delivering food born pathogen bacteria including Proteus species. Recently Proteus bacteria developed drug resistance against many antimicrobial drugs and it causes difficulty in patient’s treatment. Hence its important to indicate the rate of Proteus species, P. mirabilis and P. Vulgaris, in the meat of different animals and to find the prevalence of b-lactamase resistance genes (blaTEM-1, blaCMY, blaCMY2, blaShv, blaOXA, and blaCTX) in Proteus species. Molecular identification of Proteus bacteria was confirmed by PCR amplification of part of 16S rRNA using Proteus specific set of primers. 70 meat samples (cattle, sheep, chicken, turkey, goat, and fish) were collected in local meat shops in the center of Sulaimani city. 29 (41.4%) samples were positive to Proteus species and 22 (75.87%) isolates were P. mirabilis and seven (24.13%) were P. vulgaris based on conventional biochemical tests. The drug sensitivity test was performed for all isolates using a disk diffusion assay (Kirby Bauer test). The multidrug resistance was found in all isolates and the most common drug resistance phenotype were against tetracycline, rifampin, and doxycycline, while the imepenem, tobramycin, and meropenem remain more effective against the bacteria. Resistance genes, blaTEM-1, and blaShv were found in five isolates (17.2%) of Proteus. Three isolates (10.3%) were positive to blaTEM-1 resistance gene and two isolates (6.8%) were positive to blaShv. All resistance genes recorded in this study were recovered in P. mirabilis and none of them was reported in p. vulgaris. None of the isolates was positive to beta-lactamase genes, blaCMY, blaCMY2, blaOXA, and blaCTX.
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| format | Article |
| id | doaj-art-0e28cd81560948ce902e8f524e21381c |
| institution | Kabale University |
| issn | 2411-7684 2411-7706 |
| language | English |
| publishDate | 2019-08-01 |
| publisher | Sulaimani Polytechnic University |
| record_format | Article |
| series | Kurdistan Journal of Applied Research |
| spelling | doaj-art-0e28cd81560948ce902e8f524e21381c2025-02-09T21:00:36ZengSulaimani Polytechnic UniversityKurdistan Journal of Applied Research2411-76842411-77062019-08-014310.24017/science.2019.ICHMS.2307Prevalence and molecular characterization of Beta-lactamase resistance gene in multidrug resistance bacteria, Proteus spp.Sabiha S. Salih0Shno J. Mohammed1Imad M Noori2Lana MA Mohammed3Taib A. Hama Soor4Laboratory department, College of Health, Sulaimani Polytechnic University, Sulaimani, IraqBacteriology department, Central laboratory, Sulaimani Teaching Hospital, Sulaimani, IraqNursing department, College of Health, Sulaimani Polytechnic University, Sulaimani, IraqLaboratory department, College of Health, Sulaimani Polytechnic University, Sulaimani, IraqLaboratory department, College of Health, Sulaimani Polytechnic University, Sulaimani, IraqExisting of drug resistance bacteria in meat is a series of health concern and beta-lactamase is responsible to generate multi drug resistances in bacteria. Meat is a source of delivering food born pathogen bacteria including Proteus species. Recently Proteus bacteria developed drug resistance against many antimicrobial drugs and it causes difficulty in patient’s treatment. Hence its important to indicate the rate of Proteus species, P. mirabilis and P. Vulgaris, in the meat of different animals and to find the prevalence of b-lactamase resistance genes (blaTEM-1, blaCMY, blaCMY2, blaShv, blaOXA, and blaCTX) in Proteus species. Molecular identification of Proteus bacteria was confirmed by PCR amplification of part of 16S rRNA using Proteus specific set of primers. 70 meat samples (cattle, sheep, chicken, turkey, goat, and fish) were collected in local meat shops in the center of Sulaimani city. 29 (41.4%) samples were positive to Proteus species and 22 (75.87%) isolates were P. mirabilis and seven (24.13%) were P. vulgaris based on conventional biochemical tests. The drug sensitivity test was performed for all isolates using a disk diffusion assay (Kirby Bauer test). The multidrug resistance was found in all isolates and the most common drug resistance phenotype were against tetracycline, rifampin, and doxycycline, while the imepenem, tobramycin, and meropenem remain more effective against the bacteria. Resistance genes, blaTEM-1, and blaShv were found in five isolates (17.2%) of Proteus. Three isolates (10.3%) were positive to blaTEM-1 resistance gene and two isolates (6.8%) were positive to blaShv. All resistance genes recorded in this study were recovered in P. mirabilis and none of them was reported in p. vulgaris. None of the isolates was positive to beta-lactamase genes, blaCMY, blaCMY2, blaOXA, and blaCTX. https://kjar.spu.edu.iq/index.php/kjar/article/view/307Meat born pathogen, Proteus spp., Antibiotic sensitivity test, Resistance genes, |
| spellingShingle | Sabiha S. Salih Shno J. Mohammed Imad M Noori Lana MA Mohammed Taib A. Hama Soor Prevalence and molecular characterization of Beta-lactamase resistance gene in multidrug resistance bacteria, Proteus spp. Kurdistan Journal of Applied Research Meat born pathogen, Proteus spp., Antibiotic sensitivity test, Resistance genes, |
| title | Prevalence and molecular characterization of Beta-lactamase resistance gene in multidrug resistance bacteria, Proteus spp. |
| title_full | Prevalence and molecular characterization of Beta-lactamase resistance gene in multidrug resistance bacteria, Proteus spp. |
| title_fullStr | Prevalence and molecular characterization of Beta-lactamase resistance gene in multidrug resistance bacteria, Proteus spp. |
| title_full_unstemmed | Prevalence and molecular characterization of Beta-lactamase resistance gene in multidrug resistance bacteria, Proteus spp. |
| title_short | Prevalence and molecular characterization of Beta-lactamase resistance gene in multidrug resistance bacteria, Proteus spp. |
| title_sort | prevalence and molecular characterization of beta lactamase resistance gene in multidrug resistance bacteria proteus spp |
| topic | Meat born pathogen, Proteus spp., Antibiotic sensitivity test, Resistance genes, |
| url | https://kjar.spu.edu.iq/index.php/kjar/article/view/307 |
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