Prokaryotic cellulase gene clusters derived from 2,305 metagenomes
Abstract Cellulose is a carbon source widespread in nature. However, it is a difficult task for any organism to get carbon atoms from the cellulose as it has a highly complex structure. Only a few taxonomic groups are known to decompose cellulose. They do it by producing cellulases, the various enzy...
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Nature Portfolio
2025-02-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-04524-9 |
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| author | Bing Song Fernando D. K. Tria Josip Skejo |
| author_facet | Bing Song Fernando D. K. Tria Josip Skejo |
| author_sort | Bing Song |
| collection | DOAJ |
| description | Abstract Cellulose is a carbon source widespread in nature. However, it is a difficult task for any organism to get carbon atoms from the cellulose as it has a highly complex structure. Only a few taxonomic groups are known to decompose cellulose. They do it by producing cellulases, the various enzymes which break beta-glycosidic bonds in the cellulose. Cellulases were identified in 1,735 metagenomes from 225 bioprojects. The set of 12,837 metagenome-derived cellulases encompass three catalytic functions: exoglucanases (CBH, 1,042), endoglucanases (EG, 5,685), and beta-glucosidases (βG, 6,110). All three enzymatic functions are thought to be necessary for driving cellulase to a cascade of reactions that can make cellulose available as glucose. These metagenome-derived cellulases were clustered into protein families for each EC category individually, resulting in a total of 136 clusters, with the majority observed for EG (97 clusters), followed by βG (19 clusters) and CBH (19 clusters). These clusters provided a useful cellulase dataset for future research on cellulase utilization. |
| format | Article |
| id | doaj-art-0e799018736a48cca108a6a4eafa8800 |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
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| spelling | doaj-art-0e799018736a48cca108a6a4eafa88002025-02-09T12:11:34ZengNature PortfolioScientific Data2052-44632025-02-011211510.1038/s41597-025-04524-9Prokaryotic cellulase gene clusters derived from 2,305 metagenomesBing Song0Fernando D. K. Tria1Josip Skejo2College of Animal Science and Technology, Northwest A&F UniversityInstitute of Molecular Evolution, Heinrich-Heine University Düsseldorf, Universitätstraße 1Institute of Molecular Evolution, Heinrich-Heine University Düsseldorf, Universitätstraße 1Abstract Cellulose is a carbon source widespread in nature. However, it is a difficult task for any organism to get carbon atoms from the cellulose as it has a highly complex structure. Only a few taxonomic groups are known to decompose cellulose. They do it by producing cellulases, the various enzymes which break beta-glycosidic bonds in the cellulose. Cellulases were identified in 1,735 metagenomes from 225 bioprojects. The set of 12,837 metagenome-derived cellulases encompass three catalytic functions: exoglucanases (CBH, 1,042), endoglucanases (EG, 5,685), and beta-glucosidases (βG, 6,110). All three enzymatic functions are thought to be necessary for driving cellulase to a cascade of reactions that can make cellulose available as glucose. These metagenome-derived cellulases were clustered into protein families for each EC category individually, resulting in a total of 136 clusters, with the majority observed for EG (97 clusters), followed by βG (19 clusters) and CBH (19 clusters). These clusters provided a useful cellulase dataset for future research on cellulase utilization.https://doi.org/10.1038/s41597-025-04524-9 |
| spellingShingle | Bing Song Fernando D. K. Tria Josip Skejo Prokaryotic cellulase gene clusters derived from 2,305 metagenomes Scientific Data |
| title | Prokaryotic cellulase gene clusters derived from 2,305 metagenomes |
| title_full | Prokaryotic cellulase gene clusters derived from 2,305 metagenomes |
| title_fullStr | Prokaryotic cellulase gene clusters derived from 2,305 metagenomes |
| title_full_unstemmed | Prokaryotic cellulase gene clusters derived from 2,305 metagenomes |
| title_short | Prokaryotic cellulase gene clusters derived from 2,305 metagenomes |
| title_sort | prokaryotic cellulase gene clusters derived from 2 305 metagenomes |
| url | https://doi.org/10.1038/s41597-025-04524-9 |
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