The Effect of Culture Techniques of Hypoxic Stem Cell Secretome on The Number of Growth Factor TGF-β, BMP-2, VEGF
Background: Mesenchymal stem/stromal cell (MSC) therapy is now an effective therapeutic modality for treating various diseases. In its application, stem cells require signaling molecules, including growth factors, cytokines, and chemokines. Signaling molecules function in an orderly manner and are g...
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Universitas Airlangga
2022-04-01
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| Series: | Journal Orthopaedi and Traumatology Surabaya |
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| Online Access: | https://e-journal.unair.ac.id/JOINTS/article/view/33057 |
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| author | Mouli Edward Ferdiansyah Mahyudin Dionysius Bramta Putra Manyakori |
| author_facet | Mouli Edward Ferdiansyah Mahyudin Dionysius Bramta Putra Manyakori |
| author_sort | Mouli Edward |
| collection | DOAJ |
| description | Background: Mesenchymal stem/stromal cell (MSC) therapy is now an effective therapeutic modality for treating various diseases. In its application, stem cells require signaling molecules, including growth factors, cytokines, and chemokines. Signaling molecules function in an orderly manner and are greatly influenced by the physiological environment. Stem cell culture techniques with hypoxic conditions can produce growth factors similar to those found in fracture conditions. This study aimed to evaluate the differential expression of growth factors in cultured normoxic and hypoxic bone marrow stem cell (BMSCs).
Methods: This in vitro laboratory experimental study examined normoxic and hypoxic BMSC cultures. BMSCs were harvested from rabbits, propagated in vitro, and cultured under normoxic and hypoxic conditions. Vascular endothelial growth factor (VEGF), Transforming growth factor-β (TGF-β), and Bone morphogenetic protein-2(BMP-2) levels were measured using ELISA.
Results: VEGF, TGF-β, and BMP-2 expression showed significant differences between the normoxia and hypoxia groups. The VEGF, TGF-β, and BMP-2 expressions were higher in the hypoxia group compared with the normoxia group (p < 0.05).
Conclusion: The expression of TGF-β1, VEGF, and BMP-2 growth factors in cultured BMSCs was significantly different between normoxic and hypoxic conditions. TGF-β1, VEGF, and BMP-2 expression increased under hypoxic conditions. |
| format | Article |
| id | doaj-art-0eb362fd74434721bc46ca48a206ebd2 |
| institution | Kabale University |
| issn | 2722-712X 2460-8742 |
| language | English |
| publishDate | 2022-04-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Journal Orthopaedi and Traumatology Surabaya |
| spelling | doaj-art-0eb362fd74434721bc46ca48a206ebd22025-02-12T07:28:21ZengUniversitas AirlanggaJournal Orthopaedi and Traumatology Surabaya2722-712X2460-87422022-04-011115910.20473/joints.v11i1.2022.5-927126The Effect of Culture Techniques of Hypoxic Stem Cell Secretome on The Number of Growth Factor TGF-β, BMP-2, VEGFMouli Edward0https://orcid.org/0000-0002-1667-9770Ferdiansyah Mahyudin1https://orcid.org/0000-0001-8757-9251Dionysius Bramta Putra Manyakori2https://orcid.org/0000-0001-5713-4147Department of Orthopedics and Traumatology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; Department of Orthopedics and Traumatology, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia; Cell and Tissue Bank-Regenerative Medicine, Dr. Soetomo General Academic Hospital, Surabaya, IndonesiaDepartment of Orthopedics and Traumatology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia; Department of Orthopedics and Traumatology, Dr. Soetomo General Academic Hospital, Surabaya, Indonesia; Cell and Tissue Bank-Regenerative Medicine, Dr. Soetomo General Academic Hospital, Surabaya, IndonesiaDepartment of Orthopedics and Traumatology, Dr. Soeroto General Hospital, Ngawi, IndonesiaBackground: Mesenchymal stem/stromal cell (MSC) therapy is now an effective therapeutic modality for treating various diseases. In its application, stem cells require signaling molecules, including growth factors, cytokines, and chemokines. Signaling molecules function in an orderly manner and are greatly influenced by the physiological environment. Stem cell culture techniques with hypoxic conditions can produce growth factors similar to those found in fracture conditions. This study aimed to evaluate the differential expression of growth factors in cultured normoxic and hypoxic bone marrow stem cell (BMSCs). Methods: This in vitro laboratory experimental study examined normoxic and hypoxic BMSC cultures. BMSCs were harvested from rabbits, propagated in vitro, and cultured under normoxic and hypoxic conditions. Vascular endothelial growth factor (VEGF), Transforming growth factor-β (TGF-β), and Bone morphogenetic protein-2(BMP-2) levels were measured using ELISA. Results: VEGF, TGF-β, and BMP-2 expression showed significant differences between the normoxia and hypoxia groups. The VEGF, TGF-β, and BMP-2 expressions were higher in the hypoxia group compared with the normoxia group (p < 0.05). Conclusion: The expression of TGF-β1, VEGF, and BMP-2 growth factors in cultured BMSCs was significantly different between normoxic and hypoxic conditions. TGF-β1, VEGF, and BMP-2 expression increased under hypoxic conditions.https://e-journal.unair.ac.id/JOINTS/article/view/33057bone morphogenetic protein-2human and medicinehypoxic secretomevascular endothelial growth factortransforming growth factor-β |
| spellingShingle | Mouli Edward Ferdiansyah Mahyudin Dionysius Bramta Putra Manyakori The Effect of Culture Techniques of Hypoxic Stem Cell Secretome on The Number of Growth Factor TGF-β, BMP-2, VEGF Journal Orthopaedi and Traumatology Surabaya bone morphogenetic protein-2 human and medicine hypoxic secretome vascular endothelial growth factor transforming growth factor-β |
| title | The Effect of Culture Techniques of Hypoxic Stem Cell Secretome on The Number of Growth Factor TGF-β, BMP-2, VEGF |
| title_full | The Effect of Culture Techniques of Hypoxic Stem Cell Secretome on The Number of Growth Factor TGF-β, BMP-2, VEGF |
| title_fullStr | The Effect of Culture Techniques of Hypoxic Stem Cell Secretome on The Number of Growth Factor TGF-β, BMP-2, VEGF |
| title_full_unstemmed | The Effect of Culture Techniques of Hypoxic Stem Cell Secretome on The Number of Growth Factor TGF-β, BMP-2, VEGF |
| title_short | The Effect of Culture Techniques of Hypoxic Stem Cell Secretome on The Number of Growth Factor TGF-β, BMP-2, VEGF |
| title_sort | effect of culture techniques of hypoxic stem cell secretome on the number of growth factor tgf β bmp 2 vegf |
| topic | bone morphogenetic protein-2 human and medicine hypoxic secretome vascular endothelial growth factor transforming growth factor-β |
| url | https://e-journal.unair.ac.id/JOINTS/article/view/33057 |
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