Detection and quantification of pathogens in saliva of adolescents with cerebral palsy: a cross-sectional study
BackgroundElevated levels of inflammatory mediators in saliva have been described in individuals with cerebral palsy (CP).ObjectiveThe aim of this study was to detect and quantify the pathogens Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum and Prevotella in...
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Frontiers Media S.A.
2023-12-01
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| Series: | Frontiers in Dental Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fdmed.2023.1208243/full |
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| author | Rosemeire Arai Yoshida Tiago Bertola Lobato Renata Gorjão Lucas Santiago França Lívia Araujo Alves Maria Teresa Botti Rodrigues Santos |
| author_facet | Rosemeire Arai Yoshida Tiago Bertola Lobato Renata Gorjão Lucas Santiago França Lívia Araujo Alves Maria Teresa Botti Rodrigues Santos |
| author_sort | Rosemeire Arai Yoshida |
| collection | DOAJ |
| description | BackgroundElevated levels of inflammatory mediators in saliva have been described in individuals with cerebral palsy (CP).ObjectiveThe aim of this study was to detect and quantify the pathogens Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum and Prevotella intermedia in the saliva of adolescents with CP.MethodsThis is a cross-sectional study with adolescents with CP to detect periodontopathogens from saliva samples. Initially, saliva was collected from the CP (n = 34) and control groups (n = 31), followed by the gingival index (GI) for distribution of the groups of individuals with CP and control with gingivitis (bleeding on probing ≥ 10%) and without gingivitis. Bacterial DNA was extracted from saliva samples for detection of periodontopathogens by quantitative PCR (q-PCR). Data were analyzed by Mann–Whitney and Kruskal–Wallis tests, analysis of variance t-test (ANOVA) with Tukey–Kramer post-hoc tests (p < 0.05).ResultsThe quantification of DNA of periodontopathogens in saliva samples showed that adolescents with CP present a variability (22.93–39.56) in the detection of P. gingivalis and that some subjects with CP and gingivitis (n = 4) present high quantification of P. gingivalis (ranging 39.56–37.65), although no significant difference was found between the groups (p > 0.05). A significant contrast was observed for the pathogen P. intermedia when comparing the difference in the control group (p = 0.0396). No major differences were detected in the quantification of periodontopathogens evaluated between the control group and CP.ConclusionAdolescents with CP showed variability in the detection of DNA of periodontopathogens, especially a great variation in the detection of P gingivalis in saliva of CP with gingivitis. |
| format | Article |
| id | doaj-art-128f044c18fd4df59a63ea1492d85db7 |
| institution | Kabale University |
| issn | 2673-4915 |
| language | English |
| publishDate | 2023-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Dental Medicine |
| spelling | doaj-art-128f044c18fd4df59a63ea1492d85db72025-02-11T10:46:57ZengFrontiers Media S.A.Frontiers in Dental Medicine2673-49152023-12-01410.3389/fdmed.2023.12082431208243Detection and quantification of pathogens in saliva of adolescents with cerebral palsy: a cross-sectional studyRosemeire Arai Yoshida0Tiago Bertola Lobato1Renata Gorjão2Lucas Santiago França3Lívia Araujo Alves4Maria Teresa Botti Rodrigues Santos5Postgraduate Program in Dentistry, Department of Individuals with Special Needs, Cruzeiro do Sul University, São Paulo, BrazilPostgraduate Interdisciplinary Program of Health Sciences, Cruzeiro do Sul University, São Paulo, BrazilPostgraduate Interdisciplinary Program of Health Sciences, Cruzeiro do Sul University, São Paulo, BrazilDepartment of Dentistry, Postgraduate Program in Dentistry, Cruzeiro do Sul University, São Paulo, BrazilDepartment of Dentistry, Postgraduate Program in Dentistry, Cruzeiro do Sul University, São Paulo, BrazilPostgraduate Program in Dentistry, Department of Individuals with Special Needs, Cruzeiro do Sul University, São Paulo, BrazilBackgroundElevated levels of inflammatory mediators in saliva have been described in individuals with cerebral palsy (CP).ObjectiveThe aim of this study was to detect and quantify the pathogens Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum and Prevotella intermedia in the saliva of adolescents with CP.MethodsThis is a cross-sectional study with adolescents with CP to detect periodontopathogens from saliva samples. Initially, saliva was collected from the CP (n = 34) and control groups (n = 31), followed by the gingival index (GI) for distribution of the groups of individuals with CP and control with gingivitis (bleeding on probing ≥ 10%) and without gingivitis. Bacterial DNA was extracted from saliva samples for detection of periodontopathogens by quantitative PCR (q-PCR). Data were analyzed by Mann–Whitney and Kruskal–Wallis tests, analysis of variance t-test (ANOVA) with Tukey–Kramer post-hoc tests (p < 0.05).ResultsThe quantification of DNA of periodontopathogens in saliva samples showed that adolescents with CP present a variability (22.93–39.56) in the detection of P. gingivalis and that some subjects with CP and gingivitis (n = 4) present high quantification of P. gingivalis (ranging 39.56–37.65), although no significant difference was found between the groups (p > 0.05). A significant contrast was observed for the pathogen P. intermedia when comparing the difference in the control group (p = 0.0396). No major differences were detected in the quantification of periodontopathogens evaluated between the control group and CP.ConclusionAdolescents with CP showed variability in the detection of DNA of periodontopathogens, especially a great variation in the detection of P gingivalis in saliva of CP with gingivitis.https://www.frontiersin.org/articles/10.3389/fdmed.2023.1208243/fullcerebral palsyperiodontal diseasePorphyromonas gingivalissalivaq-PCR |
| spellingShingle | Rosemeire Arai Yoshida Tiago Bertola Lobato Renata Gorjão Lucas Santiago França Lívia Araujo Alves Maria Teresa Botti Rodrigues Santos Detection and quantification of pathogens in saliva of adolescents with cerebral palsy: a cross-sectional study Frontiers in Dental Medicine cerebral palsy periodontal disease Porphyromonas gingivalis saliva q-PCR |
| title | Detection and quantification of pathogens in saliva of adolescents with cerebral palsy: a cross-sectional study |
| title_full | Detection and quantification of pathogens in saliva of adolescents with cerebral palsy: a cross-sectional study |
| title_fullStr | Detection and quantification of pathogens in saliva of adolescents with cerebral palsy: a cross-sectional study |
| title_full_unstemmed | Detection and quantification of pathogens in saliva of adolescents with cerebral palsy: a cross-sectional study |
| title_short | Detection and quantification of pathogens in saliva of adolescents with cerebral palsy: a cross-sectional study |
| title_sort | detection and quantification of pathogens in saliva of adolescents with cerebral palsy a cross sectional study |
| topic | cerebral palsy periodontal disease Porphyromonas gingivalis saliva q-PCR |
| url | https://www.frontiersin.org/articles/10.3389/fdmed.2023.1208243/full |
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