The anticancer properties, cell-cycle cytotoxicity and apoptosis of cissus rotundifolia, trema orientalis, and buddleja polystachya with ocular applications
Background: Buddleja polystachya, Trema orientalis, and Cissus rotundifolia were applied locally for various ocular purposes, while receiving few scientific evaluations. Purpose: This study aimed to screen the anticancer properties and determine the cell-cycle cytotoxicity and apoptotic activity of...
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Elsevier
2025-02-01
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| Series: | Phytomedicine Plus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031324001258 |
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| author | Ali Hendi Alghamdi Aimun A.E. Ahmed Mahadi bashir Haidar abdalgadir Asaad khalid Ashraf N. Abdalla Mohamed E. elzubier Riyad almaimani Bassem refaat Khalid alzahrani Saleh MS. alghamdi Sheraz gul |
| author_facet | Ali Hendi Alghamdi Aimun A.E. Ahmed Mahadi bashir Haidar abdalgadir Asaad khalid Ashraf N. Abdalla Mohamed E. elzubier Riyad almaimani Bassem refaat Khalid alzahrani Saleh MS. alghamdi Sheraz gul |
| author_sort | Ali Hendi Alghamdi |
| collection | DOAJ |
| description | Background: Buddleja polystachya, Trema orientalis, and Cissus rotundifolia were applied locally for various ocular purposes, while receiving few scientific evaluations. Purpose: This study aimed to screen the anticancer properties and determine the cell-cycle cytotoxicity and apoptotic activity of the most promising plant extract. Methods: In this study, MTT assays with MCF7 (human breast adenocarcinoma), HT29 (human colorectal adenocarcinoma) and HepG2 (human liver adenocarcinoma) were used. In addition to MRC5 (normal human foetal lung fibroblast) was carried out for preliminary activity screening and selectivity. The most promising extract was subjected to GC–MS analysis to determine the phytochemical composition. Additionally, a clonogenic assay was performed to measure tumor cell survival and subsequent proliferative capacity after drug exposure was conducted for the most active extract(s) and finally western blotting was used to determine the expression change of the two selected proteins (survivin and CCND1) in order to determine the exact mechanistic features of the most promising plant extract. Results: The six extracts showed variable IC50 values ranging from 1.77 to - 40.97 μg/mL. The most active extracts were C. rotundifolia coded as (stem; BEP-03A and leaves; BEP-03B) on HepG2 cells and showed ∼ 4 and 8 fold selectivity compared to normal MRC5 cells. Both extracts showed a dose-dependent clonogenic effect on HepG2 cells, which was comparable to the effect of doxorubicin. The extract (BEP-03B) caused a significant decrease in the expression of survivin and CCND1 compared to the control GAPDH at its highest dose (12 µg/mL). The GC–MS chromatogram of the leaf of C. rotundifolia extract (BEP-03B) revealed the presence of 17 compounds, as the main phytoconstituents representing 57.5 % of the total compounds present in BEP-03B Three steroidal components (12, 14 and 15) were the main components, while compound stigmast-5-en-3-ol (compound 15) was the main component. Conclusions: Leaves of Cissus rotundifolia (Forssk.) Vahl, possess a significant cytotoxic effect and it may produce this effect, through apoptosis induction, perturbation, and disruption of the cell cycle. The detected phytoconstituents in the plant extract might be involved in the tested cytotoxic activity and its molecular apoptotic mechanism. Future studies are required to isolate the active ingredient(s) and confirm the therapeutic application(s). |
| format | Article |
| id | doaj-art-12c92d4aed21424081251d65cab02343 |
| institution | Kabale University |
| issn | 2667-0313 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
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| series | Phytomedicine Plus |
| spelling | doaj-art-12c92d4aed21424081251d65cab023432025-02-10T04:35:03ZengElsevierPhytomedicine Plus2667-03132025-02-0151100651The anticancer properties, cell-cycle cytotoxicity and apoptosis of cissus rotundifolia, trema orientalis, and buddleja polystachya with ocular applicationsAli Hendi Alghamdi0Aimun A.E. Ahmed1Mahadi bashir2Haidar abdalgadir3Asaad khalid4Ashraf N. Abdalla5Mohamed E. elzubier6Riyad almaimani7Bassem refaat8Khalid alzahrani9Saleh MS. alghamdi10Sheraz gul11Surgery Department, Faculty of Medicine, Al-Baha University, B.O. Box: 1998, Al Baha 65515, Saudi Arabia; Corresponding author.Pharmacology Department, Faculty of Medicine, Al-Baha University, B.O. Box: 1998, Al Baha 65515, Saudi Arabia; Pharmacology Department, Faculty of Pharmacy, Omdurman Islamic University, P.O. Box 2587, Khartoum 11111, SudanSurgery Department, Faculty of Medicine, Al-Baha University, B.O. Box: 1998, Al Baha 65515, Saudi ArabiaBiology Department, Faculty of Science, Al-Baha University, Al Baha 65515, Saudi ArabiaSubstance Abuse and Toxicology Research Center, Jazan University, P. O. Box: 114, Jazan 82511, Saudi ArabiaDepartment of Pharmacology and Toxicology, College of Pharmacy, Umm Al-Qura University, Makkah 21955, Saudi Arabia; Department of Pharmacology and Toxicology, National Center for Research, Medicinal and Aromatic Plants Research Institute, P.O. Box 2424, Khartoum 11111, SudanDepartment of Biochemistry, Faculty of Medicine, Umm Al-Qura University, Makkah 21955, Saudi ArabiaDepartment of Biochemistry, Faculty of Medicine, Umm Al-Qura University, Makkah 21955, Saudi ArabiaLaboratory Medicine Department, Faculty of Applied Medical Sciences, Umm Al-Qura University, Makkah 21955, Saudi ArabiaFaculty of Medicine, Al-Baha University, B.O. Box: 1998, Al Baha 65515, Saudi ArabiaFaculty of Medicine, Al-Baha University, B.O. Box: 1998, Al Baha 65515, Saudi ArabiaFraunhofer Institure for Translational Medicine and Pharmacology, Schnackenburgallee 114, Hamburg D-22525, GermanyBackground: Buddleja polystachya, Trema orientalis, and Cissus rotundifolia were applied locally for various ocular purposes, while receiving few scientific evaluations. Purpose: This study aimed to screen the anticancer properties and determine the cell-cycle cytotoxicity and apoptotic activity of the most promising plant extract. Methods: In this study, MTT assays with MCF7 (human breast adenocarcinoma), HT29 (human colorectal adenocarcinoma) and HepG2 (human liver adenocarcinoma) were used. In addition to MRC5 (normal human foetal lung fibroblast) was carried out for preliminary activity screening and selectivity. The most promising extract was subjected to GC–MS analysis to determine the phytochemical composition. Additionally, a clonogenic assay was performed to measure tumor cell survival and subsequent proliferative capacity after drug exposure was conducted for the most active extract(s) and finally western blotting was used to determine the expression change of the two selected proteins (survivin and CCND1) in order to determine the exact mechanistic features of the most promising plant extract. Results: The six extracts showed variable IC50 values ranging from 1.77 to - 40.97 μg/mL. The most active extracts were C. rotundifolia coded as (stem; BEP-03A and leaves; BEP-03B) on HepG2 cells and showed ∼ 4 and 8 fold selectivity compared to normal MRC5 cells. Both extracts showed a dose-dependent clonogenic effect on HepG2 cells, which was comparable to the effect of doxorubicin. The extract (BEP-03B) caused a significant decrease in the expression of survivin and CCND1 compared to the control GAPDH at its highest dose (12 µg/mL). The GC–MS chromatogram of the leaf of C. rotundifolia extract (BEP-03B) revealed the presence of 17 compounds, as the main phytoconstituents representing 57.5 % of the total compounds present in BEP-03B Three steroidal components (12, 14 and 15) were the main components, while compound stigmast-5-en-3-ol (compound 15) was the main component. Conclusions: Leaves of Cissus rotundifolia (Forssk.) Vahl, possess a significant cytotoxic effect and it may produce this effect, through apoptosis induction, perturbation, and disruption of the cell cycle. The detected phytoconstituents in the plant extract might be involved in the tested cytotoxic activity and its molecular apoptotic mechanism. Future studies are required to isolate the active ingredient(s) and confirm the therapeutic application(s).http://www.sciencedirect.com/science/article/pii/S2667031324001258CytotoxicityClonogenicityApoptosisCell-cycleCissus rotundifolia |
| spellingShingle | Ali Hendi Alghamdi Aimun A.E. Ahmed Mahadi bashir Haidar abdalgadir Asaad khalid Ashraf N. Abdalla Mohamed E. elzubier Riyad almaimani Bassem refaat Khalid alzahrani Saleh MS. alghamdi Sheraz gul The anticancer properties, cell-cycle cytotoxicity and apoptosis of cissus rotundifolia, trema orientalis, and buddleja polystachya with ocular applications Phytomedicine Plus Cytotoxicity Clonogenicity Apoptosis Cell-cycle Cissus rotundifolia |
| title | The anticancer properties, cell-cycle cytotoxicity and apoptosis of cissus rotundifolia, trema orientalis, and buddleja polystachya with ocular applications |
| title_full | The anticancer properties, cell-cycle cytotoxicity and apoptosis of cissus rotundifolia, trema orientalis, and buddleja polystachya with ocular applications |
| title_fullStr | The anticancer properties, cell-cycle cytotoxicity and apoptosis of cissus rotundifolia, trema orientalis, and buddleja polystachya with ocular applications |
| title_full_unstemmed | The anticancer properties, cell-cycle cytotoxicity and apoptosis of cissus rotundifolia, trema orientalis, and buddleja polystachya with ocular applications |
| title_short | The anticancer properties, cell-cycle cytotoxicity and apoptosis of cissus rotundifolia, trema orientalis, and buddleja polystachya with ocular applications |
| title_sort | anticancer properties cell cycle cytotoxicity and apoptosis of cissus rotundifolia trema orientalis and buddleja polystachya with ocular applications |
| topic | Cytotoxicity Clonogenicity Apoptosis Cell-cycle Cissus rotundifolia |
| url | http://www.sciencedirect.com/science/article/pii/S2667031324001258 |
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