Assessing the effect of beta-propiolactone inactivation on the antigenicity and immunogenicity of cluster 2.1 duck Tembusu virus
Duck Tembusu virus (DTMUV), an emerging avian pathogenic flavivirus, causes severe neurological disorders and acute egg drop syndrome in ducks. Currently, several clusters of DTMUV exhibiting significant antigenic variation are circulating in Asia with distinct geographical distributions. Therefore,...
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Elsevier
2025-03-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125001154 |
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| author | Kanana Rungprasert Wikanda Tunterek Phornphisut Areeraksakul Duangduean Prakairungnamthip Chalida Sri-in Navapon Techakriengkrai Wijit Banlunara Christine A. Jansen Teerawut Nedumpun Aunyaratana Thontiravong |
| author_facet | Kanana Rungprasert Wikanda Tunterek Phornphisut Areeraksakul Duangduean Prakairungnamthip Chalida Sri-in Navapon Techakriengkrai Wijit Banlunara Christine A. Jansen Teerawut Nedumpun Aunyaratana Thontiravong |
| author_sort | Kanana Rungprasert |
| collection | DOAJ |
| description | Duck Tembusu virus (DTMUV), an emerging avian pathogenic flavivirus, causes severe neurological disorders and acute egg drop syndrome in ducks. Currently, several clusters of DTMUV exhibiting significant antigenic variation are circulating in Asia with distinct geographical distributions. Therefore, developing vaccines based on virus clusters specific to regions is essential. To generate an effective inactivated vaccine, the virus inactivation procedure must be optimized for each virus strain. However, no information is available on the optimal inactivation protocol for cluster 2.1 DTMUV, which predominantly circulates in several Asian countries. This study aimed to determine the effect of beta-propiolactone (BPL) inactivation on the infectivity, antigenic integrity, and immunogenicity of cluster 2.1 DTMUV. Our results demonstrated that all conditions of BPL treatment (1:2000, 1:3000, and 1:4000 (vol/vol) concentration; 24, 48, and 72 h of incubation) could completely inactivate cluster 2.1 DTMUV, as evidenced by the absence of cytopathic effect (CPE) and DTMUV antigens after 3 passages in baby hamster kidney (BHK-21) cells. However, BPL at 1:4000 (vol/vol) concentration with 24 h of incubation preserved both the total protein content and the antigenic integrity of cluster 2.1 DTMUV more effectively than other conditions. Furthermore, we found that cluster 2.1 DTMUV inactivated with BPL under this condition was safe and highly immunogenic in ducks. This was evidenced by the absence of clinical signs and the robust induction of DTMUV-specific neutralizing antibodies and T helper lymphocyte responses in immunized ducks. Overall, these findings suggest that a 1:4000 dilution of BPL with 24 h of incubation is the optimal condition for complete inactivation of cluster 2.1 DTMUV without significant loss of antigenicity and immunogenicity. This protocol can serve as a guideline for efficient cluster 2.1 DTMUV inactivation, which is valuable for vaccine and immunoassay development. |
| format | Article |
| id | doaj-art-1395270de53d40f9ae8399ee12c5489b |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj-art-1395270de53d40f9ae8399ee12c5489b2025-02-07T04:46:35ZengElsevierPoultry Science0032-57912025-03-011043104878Assessing the effect of beta-propiolactone inactivation on the antigenicity and immunogenicity of cluster 2.1 duck Tembusu virusKanana Rungprasert0Wikanda Tunterek1Phornphisut Areeraksakul2Duangduean Prakairungnamthip3Chalida Sri-in4Navapon Techakriengkrai5Wijit Banlunara6Christine A. Jansen7Teerawut Nedumpun8Aunyaratana Thontiravong9Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, ThailandDepartment of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, ThailandDepartment of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, ThailandDepartment of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, ThailandCenter of Excellence in Animal Vector-Borne Disease, Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, ThailandDepartment of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, ThailandDepartment of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, ThailandCell Biology and Immunology Group, Department of Animal Sciences, Wageningen University and Research, Wageningen, the NetherlandsDepartment of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, ThailandDepartment of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand; Center of Excellence in Animal Vector-Borne Disease, Department of Veterinary Pathology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand; Center of Excellence for Emerging and Re-emerging Infectious Diseases in Animals (CUEIDAs), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand; Center of Excellence of Systems Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok, Thailand; Corresponding author: Aunyaratana Thontiravong, Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand.Duck Tembusu virus (DTMUV), an emerging avian pathogenic flavivirus, causes severe neurological disorders and acute egg drop syndrome in ducks. Currently, several clusters of DTMUV exhibiting significant antigenic variation are circulating in Asia with distinct geographical distributions. Therefore, developing vaccines based on virus clusters specific to regions is essential. To generate an effective inactivated vaccine, the virus inactivation procedure must be optimized for each virus strain. However, no information is available on the optimal inactivation protocol for cluster 2.1 DTMUV, which predominantly circulates in several Asian countries. This study aimed to determine the effect of beta-propiolactone (BPL) inactivation on the infectivity, antigenic integrity, and immunogenicity of cluster 2.1 DTMUV. Our results demonstrated that all conditions of BPL treatment (1:2000, 1:3000, and 1:4000 (vol/vol) concentration; 24, 48, and 72 h of incubation) could completely inactivate cluster 2.1 DTMUV, as evidenced by the absence of cytopathic effect (CPE) and DTMUV antigens after 3 passages in baby hamster kidney (BHK-21) cells. However, BPL at 1:4000 (vol/vol) concentration with 24 h of incubation preserved both the total protein content and the antigenic integrity of cluster 2.1 DTMUV more effectively than other conditions. Furthermore, we found that cluster 2.1 DTMUV inactivated with BPL under this condition was safe and highly immunogenic in ducks. This was evidenced by the absence of clinical signs and the robust induction of DTMUV-specific neutralizing antibodies and T helper lymphocyte responses in immunized ducks. Overall, these findings suggest that a 1:4000 dilution of BPL with 24 h of incubation is the optimal condition for complete inactivation of cluster 2.1 DTMUV without significant loss of antigenicity and immunogenicity. This protocol can serve as a guideline for efficient cluster 2.1 DTMUV inactivation, which is valuable for vaccine and immunoassay development.http://www.sciencedirect.com/science/article/pii/S0032579125001154AntigenicityBeta-propiolactoneDuck Tembusu virusImmunogenicityVirus inactivation |
| spellingShingle | Kanana Rungprasert Wikanda Tunterek Phornphisut Areeraksakul Duangduean Prakairungnamthip Chalida Sri-in Navapon Techakriengkrai Wijit Banlunara Christine A. Jansen Teerawut Nedumpun Aunyaratana Thontiravong Assessing the effect of beta-propiolactone inactivation on the antigenicity and immunogenicity of cluster 2.1 duck Tembusu virus Poultry Science Antigenicity Beta-propiolactone Duck Tembusu virus Immunogenicity Virus inactivation |
| title | Assessing the effect of beta-propiolactone inactivation on the antigenicity and immunogenicity of cluster 2.1 duck Tembusu virus |
| title_full | Assessing the effect of beta-propiolactone inactivation on the antigenicity and immunogenicity of cluster 2.1 duck Tembusu virus |
| title_fullStr | Assessing the effect of beta-propiolactone inactivation on the antigenicity and immunogenicity of cluster 2.1 duck Tembusu virus |
| title_full_unstemmed | Assessing the effect of beta-propiolactone inactivation on the antigenicity and immunogenicity of cluster 2.1 duck Tembusu virus |
| title_short | Assessing the effect of beta-propiolactone inactivation on the antigenicity and immunogenicity of cluster 2.1 duck Tembusu virus |
| title_sort | assessing the effect of beta propiolactone inactivation on the antigenicity and immunogenicity of cluster 2 1 duck tembusu virus |
| topic | Antigenicity Beta-propiolactone Duck Tembusu virus Immunogenicity Virus inactivation |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125001154 |
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