Exploring molecular disparities of H-type vasculature endothelial cells in osteonecrosis of the femoral head through single-cell analysis
Abstract Objective Recent studies highlight the role of H-type vasculature in bone regeneration. This study, based on single-cell RNA sequencing (scRNA-seq), aims to explore the changes in H-type vasculature endothelial cells (H_ECs) in osteonecrosis of the femoral head (ONFH) and hip osteoarthritis...
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| Format: | Article |
| Language: | English |
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BMC
2025-02-01
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| Series: | BMC Musculoskeletal Disorders |
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| Online Access: | https://doi.org/10.1186/s12891-024-08267-3 |
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| author | Wei Shi Dong Li Qian Xu Kai Zhang Xinyu Liang Hui Li Zhijun Li Huafeng Zhang |
| author_facet | Wei Shi Dong Li Qian Xu Kai Zhang Xinyu Liang Hui Li Zhijun Li Huafeng Zhang |
| author_sort | Wei Shi |
| collection | DOAJ |
| description | Abstract Objective Recent studies highlight the role of H-type vasculature in bone regeneration. This study, based on single-cell RNA sequencing (scRNA-seq), aims to explore the changes in H-type vasculature endothelial cells (H_ECs) in osteonecrosis of the femoral head (ONFH) and hip osteoarthritis (HOA), focusing on the death modes such as ferroptosis, pyroptosis, and parthanatos. Methods We re-analyzed the scRNA-seq data of femoral head samples publicly available in 2022. This study selected nine femoral head samples (3 each from HOA, ONFH stage 3 A, and ONFH stage 4). CD31 + EMCN + endothelial cells were classified as H_ECs. Molecular differences were assessed using Gene Ontology and KEGG analysis. Hypoxia, ferroptosis, pyroptosis, and parthanatos indices were calculated, and transcription factors were predicted using SENIC. Cell communication was analyzed with CellChat. Results After integrating the 9 samples, 14 cell types were identified: B cells, Mesenchymal stem cells, Osteoblasts, Endothelial cells, Monocytes, T cells, NK cells, Fibroblasts, Macrophages, Common myeloid progenitors, Chondrocytes, Myelocytes, Osteoclasts, and Pericytes. The number of endothelial cells and H_ECs decreased with necrosis severity. H_ECs showed higher angiogenic capacity but lower stress resistance compared to other endothelial cells. Angiogenic capacity decreased in necrotic samples, accompanied by an elevation in inflammation levels. The hypoxia index was higher, with ferroptosis increased in stage 3 A and parthanatos in stages 3 A and 4. No change was observed in pyroptosis. Cell communication analysis revealed downregulation of SLIT3-ROBO4 signaling during necrosis. Conclusion H_ECs show molecular differences compared to other endothelial cells. Ferroptosis and parthanatos contribute to the demise of H_ECs in ONFH, with pericytes and fibroblasts supporting H_EC angiogenesis. |
| format | Article |
| id | doaj-art-13de752ab61e469e9a641b0e4921176b |
| institution | Kabale University |
| issn | 1471-2474 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Musculoskeletal Disorders |
| spelling | doaj-art-13de752ab61e469e9a641b0e4921176b2025-02-09T12:04:12ZengBMCBMC Musculoskeletal Disorders1471-24742025-02-0126111010.1186/s12891-024-08267-3Exploring molecular disparities of H-type vasculature endothelial cells in osteonecrosis of the femoral head through single-cell analysisWei Shi0Dong Li1Qian Xu2Kai Zhang3Xinyu Liang4Hui Li5Zhijun Li6Huafeng Zhang7Department of Orthopedics, Tianjin Medical University General HospitalDepartment of Orthopedics, Tianjin Medical University General HospitalSchool of integrative Medicine, Tianjin University of Traditional Chinese MedicineDepartment of Orthopedics, Tianjin Medical University General HospitalDepartment of Orthopedics, Tianjin Medical University General HospitalDepartment of Orthopedics, Tianjin Medical University General HospitalDepartment of Orthopedics, Tianjin Medical University General HospitalDepartment of Orthopedics, Tianjin Medical University General HospitalAbstract Objective Recent studies highlight the role of H-type vasculature in bone regeneration. This study, based on single-cell RNA sequencing (scRNA-seq), aims to explore the changes in H-type vasculature endothelial cells (H_ECs) in osteonecrosis of the femoral head (ONFH) and hip osteoarthritis (HOA), focusing on the death modes such as ferroptosis, pyroptosis, and parthanatos. Methods We re-analyzed the scRNA-seq data of femoral head samples publicly available in 2022. This study selected nine femoral head samples (3 each from HOA, ONFH stage 3 A, and ONFH stage 4). CD31 + EMCN + endothelial cells were classified as H_ECs. Molecular differences were assessed using Gene Ontology and KEGG analysis. Hypoxia, ferroptosis, pyroptosis, and parthanatos indices were calculated, and transcription factors were predicted using SENIC. Cell communication was analyzed with CellChat. Results After integrating the 9 samples, 14 cell types were identified: B cells, Mesenchymal stem cells, Osteoblasts, Endothelial cells, Monocytes, T cells, NK cells, Fibroblasts, Macrophages, Common myeloid progenitors, Chondrocytes, Myelocytes, Osteoclasts, and Pericytes. The number of endothelial cells and H_ECs decreased with necrosis severity. H_ECs showed higher angiogenic capacity but lower stress resistance compared to other endothelial cells. Angiogenic capacity decreased in necrotic samples, accompanied by an elevation in inflammation levels. The hypoxia index was higher, with ferroptosis increased in stage 3 A and parthanatos in stages 3 A and 4. No change was observed in pyroptosis. Cell communication analysis revealed downregulation of SLIT3-ROBO4 signaling during necrosis. Conclusion H_ECs show molecular differences compared to other endothelial cells. Ferroptosis and parthanatos contribute to the demise of H_ECs in ONFH, with pericytes and fibroblasts supporting H_EC angiogenesis.https://doi.org/10.1186/s12891-024-08267-3Type H vesselsEndothelial cellscRNA-seqBioinformaticsBone |
| spellingShingle | Wei Shi Dong Li Qian Xu Kai Zhang Xinyu Liang Hui Li Zhijun Li Huafeng Zhang Exploring molecular disparities of H-type vasculature endothelial cells in osteonecrosis of the femoral head through single-cell analysis BMC Musculoskeletal Disorders Type H vessels Endothelial cell scRNA-seq Bioinformatics Bone |
| title | Exploring molecular disparities of H-type vasculature endothelial cells in osteonecrosis of the femoral head through single-cell analysis |
| title_full | Exploring molecular disparities of H-type vasculature endothelial cells in osteonecrosis of the femoral head through single-cell analysis |
| title_fullStr | Exploring molecular disparities of H-type vasculature endothelial cells in osteonecrosis of the femoral head through single-cell analysis |
| title_full_unstemmed | Exploring molecular disparities of H-type vasculature endothelial cells in osteonecrosis of the femoral head through single-cell analysis |
| title_short | Exploring molecular disparities of H-type vasculature endothelial cells in osteonecrosis of the femoral head through single-cell analysis |
| title_sort | exploring molecular disparities of h type vasculature endothelial cells in osteonecrosis of the femoral head through single cell analysis |
| topic | Type H vessels Endothelial cell scRNA-seq Bioinformatics Bone |
| url | https://doi.org/10.1186/s12891-024-08267-3 |
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