Whole-transcriptome analysis reveals the characteristics of intramuscular fat circRNA expression and its associated network in grazing yaks of different months of age under cold stress
Background: The aim of this study was to screen circRNAs related to fat deposition in yaks, and to identify candidate circRNAs for yak meat quality improvement. Six male yaks with insignificant differences in body weights were selected as test subjects, and 3 yaks (G18_IMF) were randomly slaughtered...
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Elsevier
2025-03-01
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| Series: | Electronic Journal of Biotechnology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0717345825000016 |
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| author | Yaqian Liu Yonggang Sun Yincang Han Weiqin Ding Shengwei Jin Jianyu Chen Fajie Gou |
| author_facet | Yaqian Liu Yonggang Sun Yincang Han Weiqin Ding Shengwei Jin Jianyu Chen Fajie Gou |
| author_sort | Yaqian Liu |
| collection | DOAJ |
| description | Background: The aim of this study was to screen circRNAs related to fat deposition in yaks, and to identify candidate circRNAs for yak meat quality improvement. Six male yaks with insignificant differences in body weights were selected as test subjects, and 3 yaks (G18_IMF) were randomly slaughtered at the beginning of the experiment, while the remaining 3 yaks were naturally grazed until 24 months of age (G24_IMF), and then slaughtered at the end of the experiment, and the intramuscular fat was collected from the dorsal muscle. At the end of the experiment, the yaks were slaughtered and the intramuscular fat from the back was collected for whole transcriptome sequencing. Results: The results showed that 352 differential circRNAs, 86 differential miRNAs and 3981 differential mRNAs were found. miRNAs and mRNAs network regulation maps were successfully constructed through gene expression correlation analysis and target gene prediction. Conclusions: Taking the intersection of the predicted circRNA target genes with the differential miRNAs for intramuscular fat in yaks of different months of age, we obtained two candidate ceRNA pairs that might be related to intramuscular fat deposition in yaks, and found that bta-let-7i might be related to fat deposition in yaks and might be regulated by X_85287959_85291068, and that X_85287959_85291068 could be a candidate circRNA to enhance the quality of yak meat. The results may provide a reference for further investigation of the regulatory network of intramuscular fat deposition in yak.How to cite: Liu Y, Sun Y, Han Y, et al. Whole-transcriptome analysis reveals the characteristics of intramuscular fat circRNA expression and its associated network in grazing yaks of different months of age under cold stress. Electron J Biotechnol 2025;74. https://doi.org/10.1016/j.ejbt.2024.10.004. |
| format | Article |
| id | doaj-art-1787674223674b67995979389882d53f |
| institution | Kabale University |
| issn | 0717-3458 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Electronic Journal of Biotechnology |
| spelling | doaj-art-1787674223674b67995979389882d53f2025-02-07T04:46:52ZengElsevierElectronic Journal of Biotechnology0717-34582025-03-01741118Whole-transcriptome analysis reveals the characteristics of intramuscular fat circRNA expression and its associated network in grazing yaks of different months of age under cold stressYaqian Liu0Yonggang Sun1Yincang Han2Weiqin Ding3Shengwei Jin4Jianyu Chen5Fajie Gou6Academy of Animal Husbandry and Veterinary Sciences, Qinghai University, Xining, China; Qinghai Key Laboratory of Plateau Livestock Genetic Resources Protection and Innovative Utilization, Xining, ChinaCorresponding author.; Academy of Animal Husbandry and Veterinary Sciences, Qinghai University, Xining, China; Qinghai Key Laboratory of Plateau Livestock Genetic Resources Protection and Innovative Utilization, Xining, ChinaAcademy of Animal Husbandry and Veterinary Sciences, Qinghai University, Xining, China; Qinghai Key Laboratory of Plateau Livestock Genetic Resources Protection and Innovative Utilization, Xining, ChinaAcademy of Animal Husbandry and Veterinary Sciences, Qinghai University, Xining, China; Qinghai Key Laboratory of Plateau Livestock Genetic Resources Protection and Innovative Utilization, Xining, ChinaAcademy of Animal Husbandry and Veterinary Sciences, Qinghai University, Xining, China; Qinghai Key Laboratory of Plateau Livestock Genetic Resources Protection and Innovative Utilization, Xining, ChinaAcademy of Animal Husbandry and Veterinary Sciences, Qinghai University, Xining, China; Qinghai Key Laboratory of Plateau Livestock Genetic Resources Protection and Innovative Utilization, Xining, ChinaAcademy of Animal Husbandry and Veterinary Sciences, Qinghai University, Xining, China; Qinghai Key Laboratory of Plateau Livestock Genetic Resources Protection and Innovative Utilization, Xining, ChinaBackground: The aim of this study was to screen circRNAs related to fat deposition in yaks, and to identify candidate circRNAs for yak meat quality improvement. Six male yaks with insignificant differences in body weights were selected as test subjects, and 3 yaks (G18_IMF) were randomly slaughtered at the beginning of the experiment, while the remaining 3 yaks were naturally grazed until 24 months of age (G24_IMF), and then slaughtered at the end of the experiment, and the intramuscular fat was collected from the dorsal muscle. At the end of the experiment, the yaks were slaughtered and the intramuscular fat from the back was collected for whole transcriptome sequencing. Results: The results showed that 352 differential circRNAs, 86 differential miRNAs and 3981 differential mRNAs were found. miRNAs and mRNAs network regulation maps were successfully constructed through gene expression correlation analysis and target gene prediction. Conclusions: Taking the intersection of the predicted circRNA target genes with the differential miRNAs for intramuscular fat in yaks of different months of age, we obtained two candidate ceRNA pairs that might be related to intramuscular fat deposition in yaks, and found that bta-let-7i might be related to fat deposition in yaks and might be regulated by X_85287959_85291068, and that X_85287959_85291068 could be a candidate circRNA to enhance the quality of yak meat. The results may provide a reference for further investigation of the regulatory network of intramuscular fat deposition in yak.How to cite: Liu Y, Sun Y, Han Y, et al. Whole-transcriptome analysis reveals the characteristics of intramuscular fat circRNA expression and its associated network in grazing yaks of different months of age under cold stress. Electron J Biotechnol 2025;74. https://doi.org/10.1016/j.ejbt.2024.10.004.http://www.sciencedirect.com/science/article/pii/S0717345825000016bta-let-7iceRNAcircRNACold stressGene expressionIntramuscular fat |
| spellingShingle | Yaqian Liu Yonggang Sun Yincang Han Weiqin Ding Shengwei Jin Jianyu Chen Fajie Gou Whole-transcriptome analysis reveals the characteristics of intramuscular fat circRNA expression and its associated network in grazing yaks of different months of age under cold stress Electronic Journal of Biotechnology bta-let-7i ceRNA circRNA Cold stress Gene expression Intramuscular fat |
| title | Whole-transcriptome analysis reveals the characteristics of intramuscular fat circRNA expression and its associated network in grazing yaks of different months of age under cold stress |
| title_full | Whole-transcriptome analysis reveals the characteristics of intramuscular fat circRNA expression and its associated network in grazing yaks of different months of age under cold stress |
| title_fullStr | Whole-transcriptome analysis reveals the characteristics of intramuscular fat circRNA expression and its associated network in grazing yaks of different months of age under cold stress |
| title_full_unstemmed | Whole-transcriptome analysis reveals the characteristics of intramuscular fat circRNA expression and its associated network in grazing yaks of different months of age under cold stress |
| title_short | Whole-transcriptome analysis reveals the characteristics of intramuscular fat circRNA expression and its associated network in grazing yaks of different months of age under cold stress |
| title_sort | whole transcriptome analysis reveals the characteristics of intramuscular fat circrna expression and its associated network in grazing yaks of different months of age under cold stress |
| topic | bta-let-7i ceRNA circRNA Cold stress Gene expression Intramuscular fat |
| url | http://www.sciencedirect.com/science/article/pii/S0717345825000016 |
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