CDCA7 enhances STAT3 transcriptional activity to regulate aerobic glycolysis and promote pancreatic cancer progression and gemcitabine resistance
Abstract Cell division cycle associated 7 (CDCA7) plays a role in various malignancies, especially pancreatic cancer (PC). However, its expression pattern and functional significance in PC require further research. Therefore, this study aimed to investigate CDCA7 expression levels and biological fun...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Publishing Group
2025-02-01
|
| Series: | Cell Death and Disease |
| Online Access: | https://doi.org/10.1038/s41419-025-07399-1 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823861528726601728 |
|---|---|
| author | Dijie Zheng Yazhu Deng Lu Deng Zhiwei He Xinghao Sun Yanyu Gong Binbin Shi Deqin Lu Chao Yu |
| author_facet | Dijie Zheng Yazhu Deng Lu Deng Zhiwei He Xinghao Sun Yanyu Gong Binbin Shi Deqin Lu Chao Yu |
| author_sort | Dijie Zheng |
| collection | DOAJ |
| description | Abstract Cell division cycle associated 7 (CDCA7) plays a role in various malignancies, especially pancreatic cancer (PC). However, its expression pattern and functional significance in PC require further research. Therefore, this study aimed to investigate CDCA7 expression levels and biological functions in PC using in vitro and in vivo experiments. Western blotting, immunohistochemistry, and real-time polymerase chain reaction were performed to detect CDCA7 expression in PC cells and tissues. Additionally, the biological functions of CDCA7 were assessed using cell proliferation, wound healing, and Transwell assays. CDCA7 overexpression promoted PC cell proliferation, migration, and invasion, and increased resistance to the chemotherapy drug gemcitabine, possibly through enhanced aerobic glycolysis. Additionally, immunoprecipitation assay showed that CDCA7 interacted with STAT3 protein and affected the transcriptional regulation of hexokinase 2. Conclusively, targeting CDCA7 might be a promising therapeutic strategy to increase gemcitabine sensitivity by inhibiting glycolysis in PC cells. |
| format | Article |
| id | doaj-art-1b336c788e874ad7b42ab9b459986896 |
| institution | Kabale University |
| issn | 2041-4889 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Publishing Group |
| record_format | Article |
| series | Cell Death and Disease |
| spelling | doaj-art-1b336c788e874ad7b42ab9b4599868962025-02-09T12:56:41ZengNature Publishing GroupCell Death and Disease2041-48892025-02-0116111210.1038/s41419-025-07399-1CDCA7 enhances STAT3 transcriptional activity to regulate aerobic glycolysis and promote pancreatic cancer progression and gemcitabine resistanceDijie Zheng0Yazhu Deng1Lu Deng2Zhiwei He3Xinghao Sun4Yanyu Gong5Binbin Shi6Deqin Lu7Chao Yu8School of Basic Medical Sciences, Guizhou Medical UniversitySchool of Basic Medical Sciences, Guizhou Medical UniversityDepartment of Hepatobiliary Surgery, the Affiliated Hospital of Guizhou Medical UniversityDepartment of Hepatobiliary Surgery, the Affiliated Hospital of Guizhou Medical UniversityDepartment of Hepatobiliary Surgery, the Affiliated Hospital of Guizhou Medical UniversityDepartment of Hepatobiliary Surgery, the Affiliated Hospital of Guizhou Medical UniversityDepartment of Hepatobiliary Surgery, the Affiliated Hospital of Guizhou Medical UniversitySchool of Basic Medical Sciences, Guizhou Medical UniversityDepartment of Hepatobiliary Surgery, the Affiliated Hospital of Guizhou Medical UniversityAbstract Cell division cycle associated 7 (CDCA7) plays a role in various malignancies, especially pancreatic cancer (PC). However, its expression pattern and functional significance in PC require further research. Therefore, this study aimed to investigate CDCA7 expression levels and biological functions in PC using in vitro and in vivo experiments. Western blotting, immunohistochemistry, and real-time polymerase chain reaction were performed to detect CDCA7 expression in PC cells and tissues. Additionally, the biological functions of CDCA7 were assessed using cell proliferation, wound healing, and Transwell assays. CDCA7 overexpression promoted PC cell proliferation, migration, and invasion, and increased resistance to the chemotherapy drug gemcitabine, possibly through enhanced aerobic glycolysis. Additionally, immunoprecipitation assay showed that CDCA7 interacted with STAT3 protein and affected the transcriptional regulation of hexokinase 2. Conclusively, targeting CDCA7 might be a promising therapeutic strategy to increase gemcitabine sensitivity by inhibiting glycolysis in PC cells.https://doi.org/10.1038/s41419-025-07399-1 |
| spellingShingle | Dijie Zheng Yazhu Deng Lu Deng Zhiwei He Xinghao Sun Yanyu Gong Binbin Shi Deqin Lu Chao Yu CDCA7 enhances STAT3 transcriptional activity to regulate aerobic glycolysis and promote pancreatic cancer progression and gemcitabine resistance Cell Death and Disease |
| title | CDCA7 enhances STAT3 transcriptional activity to regulate aerobic glycolysis and promote pancreatic cancer progression and gemcitabine resistance |
| title_full | CDCA7 enhances STAT3 transcriptional activity to regulate aerobic glycolysis and promote pancreatic cancer progression and gemcitabine resistance |
| title_fullStr | CDCA7 enhances STAT3 transcriptional activity to regulate aerobic glycolysis and promote pancreatic cancer progression and gemcitabine resistance |
| title_full_unstemmed | CDCA7 enhances STAT3 transcriptional activity to regulate aerobic glycolysis and promote pancreatic cancer progression and gemcitabine resistance |
| title_short | CDCA7 enhances STAT3 transcriptional activity to regulate aerobic glycolysis and promote pancreatic cancer progression and gemcitabine resistance |
| title_sort | cdca7 enhances stat3 transcriptional activity to regulate aerobic glycolysis and promote pancreatic cancer progression and gemcitabine resistance |
| url | https://doi.org/10.1038/s41419-025-07399-1 |
| work_keys_str_mv | AT dijiezheng cdca7enhancesstat3transcriptionalactivitytoregulateaerobicglycolysisandpromotepancreaticcancerprogressionandgemcitabineresistance AT yazhudeng cdca7enhancesstat3transcriptionalactivitytoregulateaerobicglycolysisandpromotepancreaticcancerprogressionandgemcitabineresistance AT ludeng cdca7enhancesstat3transcriptionalactivitytoregulateaerobicglycolysisandpromotepancreaticcancerprogressionandgemcitabineresistance AT zhiweihe cdca7enhancesstat3transcriptionalactivitytoregulateaerobicglycolysisandpromotepancreaticcancerprogressionandgemcitabineresistance AT xinghaosun cdca7enhancesstat3transcriptionalactivitytoregulateaerobicglycolysisandpromotepancreaticcancerprogressionandgemcitabineresistance AT yanyugong cdca7enhancesstat3transcriptionalactivitytoregulateaerobicglycolysisandpromotepancreaticcancerprogressionandgemcitabineresistance AT binbinshi cdca7enhancesstat3transcriptionalactivitytoregulateaerobicglycolysisandpromotepancreaticcancerprogressionandgemcitabineresistance AT deqinlu cdca7enhancesstat3transcriptionalactivitytoregulateaerobicglycolysisandpromotepancreaticcancerprogressionandgemcitabineresistance AT chaoyu cdca7enhancesstat3transcriptionalactivitytoregulateaerobicglycolysisandpromotepancreaticcancerprogressionandgemcitabineresistance |