Highly efficient assessment and selective determination of pancreatic cancer biomarker CA-19-9 using co-electrodeposited MXene-AgNPs nanocomposite
An ultrasensitive and label-free electrochemical immunosensor based on MXene-AgNPs nanocomposite is reported for pancreas cancer diagnostics. Ease of deposition, high speed, thickness of deposition and high efficiency of these biosensors are their special and fundamental advantages.For this purpose,...
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Elsevier
2025-02-01
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| Series: | Sensing and Bio-Sensing Research |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214180425000194 |
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| author | Mahsa Mirzayi Balal Khalilzadeh Sattar Akbari Nakhjavani Rahim Mohammad-Rezaei |
| author_facet | Mahsa Mirzayi Balal Khalilzadeh Sattar Akbari Nakhjavani Rahim Mohammad-Rezaei |
| author_sort | Mahsa Mirzayi |
| collection | DOAJ |
| description | An ultrasensitive and label-free electrochemical immunosensor based on MXene-AgNPs nanocomposite is reported for pancreas cancer diagnostics. Ease of deposition, high speed, thickness of deposition and high efficiency of these biosensors are their special and fundamental advantages.For this purpose, a pencil graphite electrode (PGE) was modified by silver and MXene (Ti3C2Tx (to increase efficiency and also the active surface area of PGE. Deposition of AgNPs-MXene nanocomposite then immobilization of streptavidin and biotinylated antibody on the electrode increases the sensitivity, conductivity and stability of the designed immunosensor. The electrochemical measurements in the attendance and absence of AgNPs-MXene can causing an increase in electrical currents. To examine the morphology of the developed immunosensor for the purpose of enhancing its optimal performance, the characterization methods employed include Field Emission Scanning Electron Microscopy (FE-SEM), Fourier-Transform Infrared Spectroscopy (FT-IR), and X-ray Diffraction (XRD). The results of the studies projected antibody (Ab) CA-19-9/AgNPs-MXene electrochemical immunosensor as a potential and affordable alternate of conventional methods like ELISA. The desirable LOD was recorded as 1 nU/mL. The repeatability and reproducibility were successfully inspected for ten repeated mensuration and the lowest RSD percentages were observed as about 5 % for the concentration of 1, 10 and 20 nU/mL. As a proof of the discussed results, the presented biosensor has a high sufficiency to be used in clinical laboratories to diagnose pancreatic cancer. We believe that our fabricated sensor can be the plan of diseases management program due to affordable rapid and label free detection of a targeted biomarkers. |
| format | Article |
| id | doaj-art-1e5a69b440a34282986806c24945a2f1 |
| institution | Kabale University |
| issn | 2214-1804 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensing and Bio-Sensing Research |
| spelling | doaj-art-1e5a69b440a34282986806c24945a2f12025-02-12T05:31:05ZengElsevierSensing and Bio-Sensing Research2214-18042025-02-0147100753Highly efficient assessment and selective determination of pancreatic cancer biomarker CA-19-9 using co-electrodeposited MXene-AgNPs nanocompositeMahsa Mirzayi0Balal Khalilzadeh1Sattar Akbari Nakhjavani2Rahim Mohammad-Rezaei3Biosensor Sciences and Technologies Research Center, Ardabil University of Medical Sciences, Ardabil, Iran; Analytical Chemistry Research Lab., Faculty of Basic Sciences, Azerbaijan Shahid Madani University, Tabriz, IranStem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Correspondence to: B. Khalilzadeh, Stem Cell Research Center (SCRC), Tabriz University of Medical Sciences, 51664-14766 Tabriz, Iran.Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, IranAnalytical Chemistry Research Lab., Faculty of Basic Sciences, Azerbaijan Shahid Madani University, Tabriz, Iran; Correspondence to: R. Mohammad-Rezaei, Department of Chemistry, Faculty of Basic Sciences, Azarbaijan Shahid Madani University, Tabriz, Iran.An ultrasensitive and label-free electrochemical immunosensor based on MXene-AgNPs nanocomposite is reported for pancreas cancer diagnostics. Ease of deposition, high speed, thickness of deposition and high efficiency of these biosensors are their special and fundamental advantages.For this purpose, a pencil graphite electrode (PGE) was modified by silver and MXene (Ti3C2Tx (to increase efficiency and also the active surface area of PGE. Deposition of AgNPs-MXene nanocomposite then immobilization of streptavidin and biotinylated antibody on the electrode increases the sensitivity, conductivity and stability of the designed immunosensor. The electrochemical measurements in the attendance and absence of AgNPs-MXene can causing an increase in electrical currents. To examine the morphology of the developed immunosensor for the purpose of enhancing its optimal performance, the characterization methods employed include Field Emission Scanning Electron Microscopy (FE-SEM), Fourier-Transform Infrared Spectroscopy (FT-IR), and X-ray Diffraction (XRD). The results of the studies projected antibody (Ab) CA-19-9/AgNPs-MXene electrochemical immunosensor as a potential and affordable alternate of conventional methods like ELISA. The desirable LOD was recorded as 1 nU/mL. The repeatability and reproducibility were successfully inspected for ten repeated mensuration and the lowest RSD percentages were observed as about 5 % for the concentration of 1, 10 and 20 nU/mL. As a proof of the discussed results, the presented biosensor has a high sufficiency to be used in clinical laboratories to diagnose pancreatic cancer. We believe that our fabricated sensor can be the plan of diseases management program due to affordable rapid and label free detection of a targeted biomarkers.http://www.sciencedirect.com/science/article/pii/S2214180425000194Electrochemical biosensorMXeneSilver nanoparticlesPancreatic cancerCA-19-9 |
| spellingShingle | Mahsa Mirzayi Balal Khalilzadeh Sattar Akbari Nakhjavani Rahim Mohammad-Rezaei Highly efficient assessment and selective determination of pancreatic cancer biomarker CA-19-9 using co-electrodeposited MXene-AgNPs nanocomposite Sensing and Bio-Sensing Research Electrochemical biosensor MXene Silver nanoparticles Pancreatic cancer CA-19-9 |
| title | Highly efficient assessment and selective determination of pancreatic cancer biomarker CA-19-9 using co-electrodeposited MXene-AgNPs nanocomposite |
| title_full | Highly efficient assessment and selective determination of pancreatic cancer biomarker CA-19-9 using co-electrodeposited MXene-AgNPs nanocomposite |
| title_fullStr | Highly efficient assessment and selective determination of pancreatic cancer biomarker CA-19-9 using co-electrodeposited MXene-AgNPs nanocomposite |
| title_full_unstemmed | Highly efficient assessment and selective determination of pancreatic cancer biomarker CA-19-9 using co-electrodeposited MXene-AgNPs nanocomposite |
| title_short | Highly efficient assessment and selective determination of pancreatic cancer biomarker CA-19-9 using co-electrodeposited MXene-AgNPs nanocomposite |
| title_sort | highly efficient assessment and selective determination of pancreatic cancer biomarker ca 19 9 using co electrodeposited mxene agnps nanocomposite |
| topic | Electrochemical biosensor MXene Silver nanoparticles Pancreatic cancer CA-19-9 |
| url | http://www.sciencedirect.com/science/article/pii/S2214180425000194 |
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