Pathogenic potential of ornithogenic <i>Escherichia coli</i> strains detected in the Earth's polar regions
Introduction. Pathogenic strains of Escherichia coli are an important object of surveillance within the One Health concept in the wild, agriculture and human society. Migratory bird colonies and high latitude avian colonies may be points of active intraspecies and interspecies contact between dif...
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Central Research Institute for Epidemiology
2024-12-01
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| Series: | Журнал микробиологии, эпидемиологии и иммунобиологии |
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| Online Access: | https://microbiol.crie.ru/jour/article/viewFile/18723/1550 |
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| author | Batyrbek I. Aslanov Daniil V. Azarov Maria A. Makarova Elizaveta G. Marysheva Lyudmila A. Kraeva Aleksey S. Mokhov Ekaterina A. Lebedeva Nikita E. Goncharov Natalya V. Lebedeva Dmitry A. Starikov Victoria V. Kolodzhieva Dmitry E. Polev Artemy E. Goncharov |
| author_facet | Batyrbek I. Aslanov Daniil V. Azarov Maria A. Makarova Elizaveta G. Marysheva Lyudmila A. Kraeva Aleksey S. Mokhov Ekaterina A. Lebedeva Nikita E. Goncharov Natalya V. Lebedeva Dmitry A. Starikov Victoria V. Kolodzhieva Dmitry E. Polev Artemy E. Goncharov |
| author_sort | Batyrbek I. Aslanov |
| collection | DOAJ |
| description | Introduction. Pathogenic strains of Escherichia coli are an important object of surveillance within the One Health concept in the wild, agriculture and human society.
Migratory bird colonies and high latitude avian colonies may be points of active intraspecies and interspecies contact between different animal species, accompanied by the spread of pathogens. At the same time, the phylogeography of E. coli in relation to the presence of natural foci of colibacillosis in polar regions remains virtually unstudied.
The aim of this study was to assess the pathogenic potential of E. coli strains from the polar regions of the Earth, based on the analysis of the genomes of these bacteria from typical ornithogenic ecosystems of the Arctic and Antarctic.
Materials and methods. The study used collections of E. coli isolated from ornithogenic biological material during expeditions to high latitude areas of the Arctic (archipelagos of Novaya Zemlya, Franz Josef Land, Svalbard) and Antarctic (Haswell Archipelago). 16 cultures associated with avian E. coli (12 polar and 4 temperate strains) were selected for genome-wide sequencing using BGI technology. The annotation of the genomes focused on the identification of genes for pathogenicity factors and antimicrobial resistance, as well as the identification of strains belonging to individual genetic lineages using the cgMLST method.
Results. The annotation of the genomes allowed their assignment to different sequence types in the multilocus sequencing typing and genome-wide sequencing typing schemes. The analysis of the geographical distribution of the sequence types of polar E. coli strains determined by the cgMLST method showed their global representation in geographically distant regions of the planet. For example, cgST 133718 was observed in Antarctica (strain 17_1myr) and in the UK, and sequence 11903, to which strain 32-1 from the northernmost point of Novaya Zemlya belonged, was previously identified in the USA.
All strains studied were characterized by the presence of extensive virulence. Among the pathogenicity factors identified were haemolysins A, E, F, siderophores, including the yersiniabactin gene cluster, a number of adhesion, colonization and invasion factors, as well as the thermostable enterotoxin EAST-1 and genes that characterize enteroaggregative strains of E. coli (the virulence regulator gene eilA and enteroaggregative protein (air)). One of the Arctic strains (33-1) had determinants of antibiotic resistance, in particular the extended-spectrum beta-lactamase gene TEM-1b and the Tn1721 transposon, including tetracycline resistance genes (tetA-TetR), were detected in its genome.
Conclusion. The results of the study indicate the circulation of E. coli strains with strong pathogenic potential in high-latitude Arctic and Antarctic ornithogenic ecosystems. The analysis of genomic data indicates the presence of geographically widespread genetic lineages in these regions, which justifies the importance of monitoring epidemic clones of E. coli, along with monitoring for other pathogens, in bird colonies in high-latitude areas. |
| format | Article |
| id | doaj-art-1fd4f1bb28184c22ba945f4718232e3e |
| institution | Kabale University |
| issn | 0372-9311 2686-7613 |
| language | Russian |
| publishDate | 2024-12-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Журнал микробиологии, эпидемиологии и иммунобиологии |
| spelling | doaj-art-1fd4f1bb28184c22ba945f4718232e3e2025-02-06T21:11:31ZrusCentral Research Institute for EpidemiologyЖурнал микробиологии, эпидемиологии и иммунобиологии0372-93112686-76132024-12-01101675876810.36233/0372-9311-6072796Pathogenic potential of ornithogenic <i>Escherichia coli</i> strains detected in the Earth's polar regionsBatyrbek I. Aslanov0https://orcid.org/0000-0002-6890-8096Daniil V. Azarov1https://orcid.org/0000-0003-2483-5144Maria A. Makarova2https://orcid.org/0000-0003-3600-2377Elizaveta G. Marysheva3https://orcid.org/0009-0000-8241-5290Lyudmila A. Kraeva4https://orcid.org/0000-0002-9115-3250Aleksey S. Mokhov5https://orcid.org/0000-0002-1519-5299Ekaterina A. Lebedeva6https://orcid.org/0000-0001-9547-0192Nikita E. Goncharov7https://orcid.org/0000-0002-6097-5091Natalya V. Lebedeva8https://orcid.org/0000-0003-3545-753XDmitry A. Starikov9https://orcid.org/0000-0002-3721-8615Victoria V. Kolodzhieva10https://orcid.org/0000-0002-1537-211XDmitry E. Polev11https://orcid.org/0000-0001-9679-2791Artemy E. Goncharov12https://orcid.org/0000-0002-5206-6656North-Western State Medical University named after I.I. MechnikovNorth-Western State Medical University named after I.I. MechnikovNorth-Western State Medical University named after I.I. MechnikovSaint Petersburg State UniversitySaint Petersburg Pasteur InstituteNorth-Western State Medical University named after I.I. MechnikovNorth-Western State Medical University named after I.I. MechnikovSaint Petersburg Pasteur InstituteMurmansk Marine Biological InstituteNizhne-Svirsky State Nature ReserveNorth-Western State Medical University named after I.I. MechnikovSaint Petersburg Pasteur InstituteNorth-Western State Medical University named after I.I. MechnikovIntroduction. Pathogenic strains of Escherichia coli are an important object of surveillance within the One Health concept in the wild, agriculture and human society. Migratory bird colonies and high latitude avian colonies may be points of active intraspecies and interspecies contact between different animal species, accompanied by the spread of pathogens. At the same time, the phylogeography of E. coli in relation to the presence of natural foci of colibacillosis in polar regions remains virtually unstudied. The aim of this study was to assess the pathogenic potential of E. coli strains from the polar regions of the Earth, based on the analysis of the genomes of these bacteria from typical ornithogenic ecosystems of the Arctic and Antarctic. Materials and methods. The study used collections of E. coli isolated from ornithogenic biological material during expeditions to high latitude areas of the Arctic (archipelagos of Novaya Zemlya, Franz Josef Land, Svalbard) and Antarctic (Haswell Archipelago). 16 cultures associated with avian E. coli (12 polar and 4 temperate strains) were selected for genome-wide sequencing using BGI technology. The annotation of the genomes focused on the identification of genes for pathogenicity factors and antimicrobial resistance, as well as the identification of strains belonging to individual genetic lineages using the cgMLST method. Results. The annotation of the genomes allowed their assignment to different sequence types in the multilocus sequencing typing and genome-wide sequencing typing schemes. The analysis of the geographical distribution of the sequence types of polar E. coli strains determined by the cgMLST method showed their global representation in geographically distant regions of the planet. For example, cgST 133718 was observed in Antarctica (strain 17_1myr) and in the UK, and sequence 11903, to which strain 32-1 from the northernmost point of Novaya Zemlya belonged, was previously identified in the USA. All strains studied were characterized by the presence of extensive virulence. Among the pathogenicity factors identified were haemolysins A, E, F, siderophores, including the yersiniabactin gene cluster, a number of adhesion, colonization and invasion factors, as well as the thermostable enterotoxin EAST-1 and genes that characterize enteroaggregative strains of E. coli (the virulence regulator gene eilA and enteroaggregative protein (air)). One of the Arctic strains (33-1) had determinants of antibiotic resistance, in particular the extended-spectrum beta-lactamase gene TEM-1b and the Tn1721 transposon, including tetracycline resistance genes (tetA-TetR), were detected in its genome. Conclusion. The results of the study indicate the circulation of E. coli strains with strong pathogenic potential in high-latitude Arctic and Antarctic ornithogenic ecosystems. The analysis of genomic data indicates the presence of geographically widespread genetic lineages in these regions, which justifies the importance of monitoring epidemic clones of E. coli, along with monitoring for other pathogens, in bird colonies in high-latitude areas.https://microbiol.crie.ru/jour/article/viewFile/18723/1550arcticantarcticaescherichia coligenome sequencingpathogenicity factorsornithogenic ecosystems |
| spellingShingle | Batyrbek I. Aslanov Daniil V. Azarov Maria A. Makarova Elizaveta G. Marysheva Lyudmila A. Kraeva Aleksey S. Mokhov Ekaterina A. Lebedeva Nikita E. Goncharov Natalya V. Lebedeva Dmitry A. Starikov Victoria V. Kolodzhieva Dmitry E. Polev Artemy E. Goncharov Pathogenic potential of ornithogenic <i>Escherichia coli</i> strains detected in the Earth's polar regions Журнал микробиологии, эпидемиологии и иммунобиологии arctic antarctica escherichia coli genome sequencing pathogenicity factors ornithogenic ecosystems |
| title | Pathogenic potential of ornithogenic <i>Escherichia coli</i> strains detected in the Earth's polar regions |
| title_full | Pathogenic potential of ornithogenic <i>Escherichia coli</i> strains detected in the Earth's polar regions |
| title_fullStr | Pathogenic potential of ornithogenic <i>Escherichia coli</i> strains detected in the Earth's polar regions |
| title_full_unstemmed | Pathogenic potential of ornithogenic <i>Escherichia coli</i> strains detected in the Earth's polar regions |
| title_short | Pathogenic potential of ornithogenic <i>Escherichia coli</i> strains detected in the Earth's polar regions |
| title_sort | pathogenic potential of ornithogenic i escherichia coli i strains detected in the earth s polar regions |
| topic | arctic antarctica escherichia coli genome sequencing pathogenicity factors ornithogenic ecosystems |
| url | https://microbiol.crie.ru/jour/article/viewFile/18723/1550 |
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