Spireoside Controls Blast Disease by Disrupting Membrane Integrity of Magnaporthe oryzae
The application of fungicides is an effective strategy for controlling plant diseases. Among these agents, plant-derived antifungal metabolites are particularly promising due to their eco-friendly and sustainable nature. Plant secondary metabolites typically exhibit broad-spectrum antifungal activit...
Saved in:
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Rice Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S167263082400091X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823856807430324224 |
|---|---|
| author | Xu Liting He Kaiwei Guo Chunyu Quan Cantao Ma Yahuan Zhang Wei Ren Lifen Wang Long Song Li Ouyang Qing Yin Junjie Zhu Xiaobo Tang Yongyan He Min Chen Xuewei Li Weitao |
| author_facet | Xu Liting He Kaiwei Guo Chunyu Quan Cantao Ma Yahuan Zhang Wei Ren Lifen Wang Long Song Li Ouyang Qing Yin Junjie Zhu Xiaobo Tang Yongyan He Min Chen Xuewei Li Weitao |
| author_sort | Xu Liting |
| collection | DOAJ |
| description | The application of fungicides is an effective strategy for controlling plant diseases. Among these agents, plant-derived antifungal metabolites are particularly promising due to their eco-friendly and sustainable nature. Plant secondary metabolites typically exhibit broad-spectrum antifungal activity without selective toxicity against pathogens. However, only a small fraction of antifungal metabolites have been identified from the tens of thousands of known plant secondary metabolites. In this study, we conducted a metabolomic analysis on both blast-resistant (Digu) and -susceptible (Lijiangxintuanheigu) rice varieties to uncover novel metabolites that enhance blast resistance. We found that 24 and 48 h post-inoculation with Magnaporthe oryzae were critical time points for metabolomic profiling, based on the infected status of M. oryzae in rice and the observed differences in shikimate accumulation between the two varieties. Following metabolomic analysis, we identified nine flavonoids that were differentially accumulated and are considered potential candidates for disease control. Among these, apigenin-7-glucoside, rhamnetin, and spireoside were found to be effective in controlling blast disease, with spireoside demonstrating the most pronounced efficacy. We discovered that spireoside controlled blast disease by inhibiting both spore germination and appressorium formation in M. oryzae, primarily through disrupting cell membrane integrity. However, spireoside did not induce rice immunity. Furthermore, spireoside was also effective in controlling sheath blight disease. Thus, spireoside shows considerable promise as a candidate for the development of a fungicide for controlling plant diseases. |
| format | Article |
| id | doaj-art-203d39b001394ad8b9bcc23f68183501 |
| institution | Kabale University |
| issn | 1672-6308 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Rice Science |
| spelling | doaj-art-203d39b001394ad8b9bcc23f681835012025-02-12T05:30:49ZengElsevierRice Science1672-63082025-01-01321107117Spireoside Controls Blast Disease by Disrupting Membrane Integrity of Magnaporthe oryzaeXu Liting0He Kaiwei1Guo Chunyu2Quan Cantao3Ma Yahuan4Zhang Wei5Ren Lifen6Wang Long7Song Li8Ouyang Qing9Yin Junjie10Zhu Xiaobo11Tang Yongyan12He Min13Chen Xuewei14Li Weitao15State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaCorresponding author:; State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, ChinaThe application of fungicides is an effective strategy for controlling plant diseases. Among these agents, plant-derived antifungal metabolites are particularly promising due to their eco-friendly and sustainable nature. Plant secondary metabolites typically exhibit broad-spectrum antifungal activity without selective toxicity against pathogens. However, only a small fraction of antifungal metabolites have been identified from the tens of thousands of known plant secondary metabolites. In this study, we conducted a metabolomic analysis on both blast-resistant (Digu) and -susceptible (Lijiangxintuanheigu) rice varieties to uncover novel metabolites that enhance blast resistance. We found that 24 and 48 h post-inoculation with Magnaporthe oryzae were critical time points for metabolomic profiling, based on the infected status of M. oryzae in rice and the observed differences in shikimate accumulation between the two varieties. Following metabolomic analysis, we identified nine flavonoids that were differentially accumulated and are considered potential candidates for disease control. Among these, apigenin-7-glucoside, rhamnetin, and spireoside were found to be effective in controlling blast disease, with spireoside demonstrating the most pronounced efficacy. We discovered that spireoside controlled blast disease by inhibiting both spore germination and appressorium formation in M. oryzae, primarily through disrupting cell membrane integrity. However, spireoside did not induce rice immunity. Furthermore, spireoside was also effective in controlling sheath blight disease. Thus, spireoside shows considerable promise as a candidate for the development of a fungicide for controlling plant diseases.http://www.sciencedirect.com/science/article/pii/S167263082400091Xappressorium formationblast diseasemembrane integrityresistancespireosidespore germination |
| spellingShingle | Xu Liting He Kaiwei Guo Chunyu Quan Cantao Ma Yahuan Zhang Wei Ren Lifen Wang Long Song Li Ouyang Qing Yin Junjie Zhu Xiaobo Tang Yongyan He Min Chen Xuewei Li Weitao Spireoside Controls Blast Disease by Disrupting Membrane Integrity of Magnaporthe oryzae Rice Science appressorium formation blast disease membrane integrity resistance spireoside spore germination |
| title | Spireoside Controls Blast Disease by Disrupting Membrane Integrity of Magnaporthe oryzae |
| title_full | Spireoside Controls Blast Disease by Disrupting Membrane Integrity of Magnaporthe oryzae |
| title_fullStr | Spireoside Controls Blast Disease by Disrupting Membrane Integrity of Magnaporthe oryzae |
| title_full_unstemmed | Spireoside Controls Blast Disease by Disrupting Membrane Integrity of Magnaporthe oryzae |
| title_short | Spireoside Controls Blast Disease by Disrupting Membrane Integrity of Magnaporthe oryzae |
| title_sort | spireoside controls blast disease by disrupting membrane integrity of magnaporthe oryzae |
| topic | appressorium formation blast disease membrane integrity resistance spireoside spore germination |
| url | http://www.sciencedirect.com/science/article/pii/S167263082400091X |
| work_keys_str_mv | AT xuliting spireosidecontrolsblastdiseasebydisruptingmembraneintegrityofmagnaportheoryzae AT hekaiwei spireosidecontrolsblastdiseasebydisruptingmembraneintegrityofmagnaportheoryzae AT guochunyu spireosidecontrolsblastdiseasebydisruptingmembraneintegrityofmagnaportheoryzae AT quancantao spireosidecontrolsblastdiseasebydisruptingmembraneintegrityofmagnaportheoryzae AT mayahuan spireosidecontrolsblastdiseasebydisruptingmembraneintegrityofmagnaportheoryzae AT zhangwei spireosidecontrolsblastdiseasebydisruptingmembraneintegrityofmagnaportheoryzae AT renlifen spireosidecontrolsblastdiseasebydisruptingmembraneintegrityofmagnaportheoryzae AT wanglong spireosidecontrolsblastdiseasebydisruptingmembraneintegrityofmagnaportheoryzae AT songli spireosidecontrolsblastdiseasebydisruptingmembraneintegrityofmagnaportheoryzae AT ouyangqing spireosidecontrolsblastdiseasebydisruptingmembraneintegrityofmagnaportheoryzae AT yinjunjie spireosidecontrolsblastdiseasebydisruptingmembraneintegrityofmagnaportheoryzae AT zhuxiaobo spireosidecontrolsblastdiseasebydisruptingmembraneintegrityofmagnaportheoryzae AT tangyongyan spireosidecontrolsblastdiseasebydisruptingmembraneintegrityofmagnaportheoryzae AT hemin spireosidecontrolsblastdiseasebydisruptingmembraneintegrityofmagnaportheoryzae AT chenxuewei spireosidecontrolsblastdiseasebydisruptingmembraneintegrityofmagnaportheoryzae AT liweitao spireosidecontrolsblastdiseasebydisruptingmembraneintegrityofmagnaportheoryzae |