An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids
The enzyme-linked immunosorbent assay (ELISA) detects antigen-antibody interactions by using enzyme-labelled conjugates and enzyme substrates that generate colour changes. This review aims to provide an overview of ELISA, its various types, and its applications in detecting metabolites in biological...
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| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2025-02-01
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| Series: | Journal of International Medical Research |
| Online Access: | https://doi.org/10.1177/03000605251315913 |
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| _version_ | 1823864060104409088 |
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| author | Suleyman Aydin Elif Emre Kader Ugur Mustafa Ata Aydin İbrahim Sahin Vedat Cinar Taner Akbulut |
| author_facet | Suleyman Aydin Elif Emre Kader Ugur Mustafa Ata Aydin İbrahim Sahin Vedat Cinar Taner Akbulut |
| author_sort | Suleyman Aydin |
| collection | DOAJ |
| description | The enzyme-linked immunosorbent assay (ELISA) detects antigen-antibody interactions by using enzyme-labelled conjugates and enzyme substrates that generate colour changes. This review aims to provide an overview of ELISA, its various types, and its applications in detecting metabolites in biological fluids. The article discusses the history of the assay, its underlying principles and procedures, common ELISA protocols, and the most accurate and reliable techniques for measuring peptide molecules in biological fluids. Additionally, we emphasize best laboratory practices to achieve consistent, high-quality results and outline the essential materials for setting up an ELISA laboratory, drawing from our over 30 years of experience in the field. |
| format | Article |
| id | doaj-art-21240b65008242dcbce08d3d1a7a1ec9 |
| institution | Kabale University |
| issn | 1473-2300 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Journal of International Medical Research |
| spelling | doaj-art-21240b65008242dcbce08d3d1a7a1ec92025-02-09T06:03:19ZengSAGE PublishingJournal of International Medical Research1473-23002025-02-015310.1177/03000605251315913An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluidsSuleyman AydinElif EmreKader UgurMustafa Ata Aydinİbrahim SahinVedat CinarTaner AkbulutThe enzyme-linked immunosorbent assay (ELISA) detects antigen-antibody interactions by using enzyme-labelled conjugates and enzyme substrates that generate colour changes. This review aims to provide an overview of ELISA, its various types, and its applications in detecting metabolites in biological fluids. The article discusses the history of the assay, its underlying principles and procedures, common ELISA protocols, and the most accurate and reliable techniques for measuring peptide molecules in biological fluids. Additionally, we emphasize best laboratory practices to achieve consistent, high-quality results and outline the essential materials for setting up an ELISA laboratory, drawing from our over 30 years of experience in the field.https://doi.org/10.1177/03000605251315913 |
| spellingShingle | Suleyman Aydin Elif Emre Kader Ugur Mustafa Ata Aydin İbrahim Sahin Vedat Cinar Taner Akbulut An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids Journal of International Medical Research |
| title | An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids |
| title_full | An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids |
| title_fullStr | An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids |
| title_full_unstemmed | An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids |
| title_short | An overview of ELISA: a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids |
| title_sort | overview of elisa a review and update on best laboratory practices for quantifying peptides and proteins in biological fluids |
| url | https://doi.org/10.1177/03000605251315913 |
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