Chlorella’s transport inhibition: A powerful defense against high ammonium stress
Ammonium (NH₄⁺) is a primary nitrogen source for many species, yet NH₄⁺-rich wastewater presents a substantial risk to environment. Chlorella sorokiniana is widely recognized for wastewater treatment. The development of high NH₄⁺ tolerant strains has the potential to significantly enhance wastewater...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651324015367 |
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| author | Lihe Xu Li Chen Longxing Jiang Jingni Zhang Peike Wu Wenguo Wang |
| author_facet | Lihe Xu Li Chen Longxing Jiang Jingni Zhang Peike Wu Wenguo Wang |
| author_sort | Lihe Xu |
| collection | DOAJ |
| description | Ammonium (NH₄⁺) is a primary nitrogen source for many species, yet NH₄⁺-rich wastewater presents a substantial risk to environment. Chlorella sorokiniana is widely recognized for wastewater treatment. The development of high NH₄⁺ tolerant strains has the potential to significantly enhance wastewater treatment efficiency and reduce treatment costs. This study reports the identification of a C. sorokiniana strain designated hact (high ammonium concentration tolerance). This strain demonstrates a remarkable tolerance to NH₄⁺ (1000 mg/L). Integrative analyses of physiology, metabolomics, and transcriptomics demonstrated that transport inhibition is the principal resistance mechanism against high NH₄⁺ stress in C. sorokiniana. Notably, under elevated NH₄⁺ conditions, the hact strain maintained robust intracellular homeostasis. In contrast, the wild-type (WT) strain exhibited suppressed metabolic activity, reactive oxygen species (ROS), and an excess of detrimental metabolites such as amines. This research enriches our understanding of microalgal molecular responses to high NH₄⁺ stress, paving the way for the development of engineered optimization strategies for microalgal bioremediation systems treating NH₄⁺-rich wastewater. |
| format | Article |
| id | doaj-art-224f6a22f83842ecaf43b6e33da0309e |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-224f6a22f83842ecaf43b6e33da0309e2025-02-12T05:29:23ZengElsevierEcotoxicology and Environmental Safety0147-65132025-01-01290117460Chlorella’s transport inhibition: A powerful defense against high ammonium stressLihe Xu0Li Chen1Longxing Jiang2Jingni Zhang3Peike Wu4Wenguo Wang5Biogas Institute of Ministry of Agriculture and Rural Affairs, Chengdu 610041, China; Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture and Rural Affairs, Chengdu 610041, ChinaBiogas Institute of Ministry of Agriculture and Rural Affairs, Chengdu 610041, China; Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture and Rural Affairs, Chengdu 610041, ChinaBiogas Institute of Ministry of Agriculture and Rural Affairs, Chengdu 610041, China; Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture and Rural Affairs, Chengdu 610041, ChinaBiogas Institute of Ministry of Agriculture and Rural Affairs, Chengdu 610041, China; Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture and Rural Affairs, Chengdu 610041, ChinaBiogas Institute of Ministry of Agriculture and Rural Affairs, Chengdu 610041, China; Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture and Rural Affairs, Chengdu 610041, ChinaBiogas Institute of Ministry of Agriculture and Rural Affairs, Chengdu 610041, China; Key Laboratory of Development and Application of Rural Renewable Energy, Ministry of Agriculture and Rural Affairs, Chengdu 610041, China; Corresponding author at: Biogas Institute of Ministry of Agriculture and Rural Affairs, Chengdu 610041, China.Ammonium (NH₄⁺) is a primary nitrogen source for many species, yet NH₄⁺-rich wastewater presents a substantial risk to environment. Chlorella sorokiniana is widely recognized for wastewater treatment. The development of high NH₄⁺ tolerant strains has the potential to significantly enhance wastewater treatment efficiency and reduce treatment costs. This study reports the identification of a C. sorokiniana strain designated hact (high ammonium concentration tolerance). This strain demonstrates a remarkable tolerance to NH₄⁺ (1000 mg/L). Integrative analyses of physiology, metabolomics, and transcriptomics demonstrated that transport inhibition is the principal resistance mechanism against high NH₄⁺ stress in C. sorokiniana. Notably, under elevated NH₄⁺ conditions, the hact strain maintained robust intracellular homeostasis. In contrast, the wild-type (WT) strain exhibited suppressed metabolic activity, reactive oxygen species (ROS), and an excess of detrimental metabolites such as amines. This research enriches our understanding of microalgal molecular responses to high NH₄⁺ stress, paving the way for the development of engineered optimization strategies for microalgal bioremediation systems treating NH₄⁺-rich wastewater.http://www.sciencedirect.com/science/article/pii/S0147651324015367C. sorokinianaHigh NH4+ concentration toleranceUptake of NH4+Transport inhibitionNH₄⁺-rich wastewater |
| spellingShingle | Lihe Xu Li Chen Longxing Jiang Jingni Zhang Peike Wu Wenguo Wang Chlorella’s transport inhibition: A powerful defense against high ammonium stress Ecotoxicology and Environmental Safety C. sorokiniana High NH4+ concentration tolerance Uptake of NH4+ Transport inhibition NH₄⁺-rich wastewater |
| title | Chlorella’s transport inhibition: A powerful defense against high ammonium stress |
| title_full | Chlorella’s transport inhibition: A powerful defense against high ammonium stress |
| title_fullStr | Chlorella’s transport inhibition: A powerful defense against high ammonium stress |
| title_full_unstemmed | Chlorella’s transport inhibition: A powerful defense against high ammonium stress |
| title_short | Chlorella’s transport inhibition: A powerful defense against high ammonium stress |
| title_sort | chlorella s transport inhibition a powerful defense against high ammonium stress |
| topic | C. sorokiniana High NH4+ concentration tolerance Uptake of NH4+ Transport inhibition NH₄⁺-rich wastewater |
| url | http://www.sciencedirect.com/science/article/pii/S0147651324015367 |
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