Crotalaria madurensis flavonol glycosides’ antibacterial activity against Staphylococcus aureus
Abstract Methicillin-resistant Staphylococcus aureus (MRSA) infections are prevalent in hospitals and often lead to significant health complications. This study aimed to explore the chemical composition of the aerial part of Crotalaria madurensis and evaluate its antioxidant and antibacterial proper...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2024-11-01
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| Series: | AMB Express |
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| Online Access: | https://doi.org/10.1186/s13568-024-01776-3 |
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| author | Hala Sh. Mohammed Salwa A. Abu El Wafa Mona H. Ibrahim Rasha Mohammad Fathy Noha A. Seif-Eldein |
| author_facet | Hala Sh. Mohammed Salwa A. Abu El Wafa Mona H. Ibrahim Rasha Mohammad Fathy Noha A. Seif-Eldein |
| author_sort | Hala Sh. Mohammed |
| collection | DOAJ |
| description | Abstract Methicillin-resistant Staphylococcus aureus (MRSA) infections are prevalent in hospitals and often lead to significant health complications. This study aimed to explore the chemical composition of the aerial part of Crotalaria madurensis and evaluate its antioxidant and antibacterial properties. The impact of gamma irradiation on the antibacterial properties of the plant extract and metabolite 1 against MRSA was also examined. Fourier-transform infrared (FTIR) analysis was conducted on the filtrates of untreated MRSA and MRSA treated with the plant extract and metabolite 1. Four flavonol glycosides were identified as gossypetin 8-methoxy, 3-O-β-D-xylopyranoside (metabolite 1), gossypetin 8-O-β-D-glucopyranoside (metabolite 2), kaempferol 3-O-β-D-glucpyranoside (Astragalin, metabolite 3), and herbacetin 7-methoxy-3-O-β-D-glucopyranoside (metabolite 4). All metabolites exhibited significant antioxidant properties using different assays. The antibacterial efficacy of the extract and metabolite 1, which showed substantial antioxidant properties compared to the other isolated metabolites, was evaluated. Both the plant extract and metabolite 1 significantly reduced the viability and cell count of MRSA at concentrations of 1.0 and 0.5 mg/ml. The antibacterial activity of the plant extract and metabolite 1 was assessed after gamma irradiation at 50 and 100 Gy, which did not significantly affect the antibacterial efficiency. FTIR analysis indicated that the plant extract and metabolite 1 significantly altered the band frequency values, bandwidth, and peak intensity % of the treated MRSA filtrate. Molecular docking studies suggested that metabolite 1 exhibited the highest antioxidant and anti-MRSA activity, with strong binding scores like the ligand, indicating an effective interaction and high affinity between metabolite 1 and the target molecule. |
| format | Article |
| id | doaj-art-227b44a2c5d34f209bb05da6b2a4c244 |
| institution | Kabale University |
| issn | 2191-0855 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | AMB Express |
| spelling | doaj-art-227b44a2c5d34f209bb05da6b2a4c2442025-02-09T12:55:52ZengSpringerOpenAMB Express2191-08552024-11-0114111910.1186/s13568-024-01776-3Crotalaria madurensis flavonol glycosides’ antibacterial activity against Staphylococcus aureusHala Sh. Mohammed0Salwa A. Abu El Wafa1Mona H. Ibrahim2Rasha Mohammad Fathy3Noha A. Seif-Eldein4Pharmacognosy and Medicinal Plants Department, Faculty of Pharmacy, Al-Azhar UniversityPharmacognosy and Medicinal Plants Department, Faculty of Pharmacy, Al-Azhar UniversityDepartment of Pharmaceutical Medicinal Chemistry and Drug Design, Faculty of Pharmacy (Girls), Al-Azhar UniversityDrug Radiation Research Department, National Center for Radiation Research and Technology (NCRRT), Egyptian Atomic Energy AuthorityPharmacognosy and Medicinal Plants Department, Faculty of Pharmacy, Al-Azhar UniversityAbstract Methicillin-resistant Staphylococcus aureus (MRSA) infections are prevalent in hospitals and often lead to significant health complications. This study aimed to explore the chemical composition of the aerial part of Crotalaria madurensis and evaluate its antioxidant and antibacterial properties. The impact of gamma irradiation on the antibacterial properties of the plant extract and metabolite 1 against MRSA was also examined. Fourier-transform infrared (FTIR) analysis was conducted on the filtrates of untreated MRSA and MRSA treated with the plant extract and metabolite 1. Four flavonol glycosides were identified as gossypetin 8-methoxy, 3-O-β-D-xylopyranoside (metabolite 1), gossypetin 8-O-β-D-glucopyranoside (metabolite 2), kaempferol 3-O-β-D-glucpyranoside (Astragalin, metabolite 3), and herbacetin 7-methoxy-3-O-β-D-glucopyranoside (metabolite 4). All metabolites exhibited significant antioxidant properties using different assays. The antibacterial efficacy of the extract and metabolite 1, which showed substantial antioxidant properties compared to the other isolated metabolites, was evaluated. Both the plant extract and metabolite 1 significantly reduced the viability and cell count of MRSA at concentrations of 1.0 and 0.5 mg/ml. The antibacterial activity of the plant extract and metabolite 1 was assessed after gamma irradiation at 50 and 100 Gy, which did not significantly affect the antibacterial efficiency. FTIR analysis indicated that the plant extract and metabolite 1 significantly altered the band frequency values, bandwidth, and peak intensity % of the treated MRSA filtrate. Molecular docking studies suggested that metabolite 1 exhibited the highest antioxidant and anti-MRSA activity, with strong binding scores like the ligand, indicating an effective interaction and high affinity between metabolite 1 and the target molecule.https://doi.org/10.1186/s13568-024-01776-3AntioxidantCrotalaria madurensisFlavonolMRSAAntibacterialDocking study |
| spellingShingle | Hala Sh. Mohammed Salwa A. Abu El Wafa Mona H. Ibrahim Rasha Mohammad Fathy Noha A. Seif-Eldein Crotalaria madurensis flavonol glycosides’ antibacterial activity against Staphylococcus aureus AMB Express Antioxidant Crotalaria madurensis Flavonol MRSA Antibacterial Docking study |
| title | Crotalaria madurensis flavonol glycosides’ antibacterial activity against Staphylococcus aureus |
| title_full | Crotalaria madurensis flavonol glycosides’ antibacterial activity against Staphylococcus aureus |
| title_fullStr | Crotalaria madurensis flavonol glycosides’ antibacterial activity against Staphylococcus aureus |
| title_full_unstemmed | Crotalaria madurensis flavonol glycosides’ antibacterial activity against Staphylococcus aureus |
| title_short | Crotalaria madurensis flavonol glycosides’ antibacterial activity against Staphylococcus aureus |
| title_sort | crotalaria madurensis flavonol glycosides antibacterial activity against staphylococcus aureus |
| topic | Antioxidant Crotalaria madurensis Flavonol MRSA Antibacterial Docking study |
| url | https://doi.org/10.1186/s13568-024-01776-3 |
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