Genome wide identification of Dof transcription factors in Carmine radish reveals RsDof33 role in cadmium stress and anthocyanin biosynthesis
Abstract Carmine radish (Raphanus sativus L.) is cultivated in Fuling, Chongqing, for its red color. Dof-TFs are critical in regulating plant growth, development, stress responses, and signal transduction.This work comprehensively examined the structure, evolution, and expression of the carmine radi...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2025-02-01
|
| Series: | Scientific Reports |
| Subjects: | |
| Online Access: | https://doi.org/10.1038/s41598-025-88308-6 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823862284660768768 |
|---|---|
| author | Caiming Gou Jia Li Bo Chen Guoting Cheng Zhangfei Zheng Hua Peng Ahmed H. El-Sappah |
| author_facet | Caiming Gou Jia Li Bo Chen Guoting Cheng Zhangfei Zheng Hua Peng Ahmed H. El-Sappah |
| author_sort | Caiming Gou |
| collection | DOAJ |
| description | Abstract Carmine radish (Raphanus sativus L.) is cultivated in Fuling, Chongqing, for its red color. Dof-TFs are critical in regulating plant growth, development, stress responses, and signal transduction.This work comprehensively examined the structure, evolution, and expression of the carmine radish Dof gene and its behavior under cadmium (Cd) stress. The radish genome has 59 RsDofs, which are divided into nine clusters (A: 8, B1: 10, B2: 10, C1: 3, C2.1: 5, C2.2: 4, C3: 11, D1: 4, and D2: 4). Phylogenetic tree analysis revealed significant Dof gene family resemblance between Arabidopsis thaliana and Brassica napus. Perhaps segment duplication resulted in RsDof gene family expansion. Cd stress-induced RsDof expression patterns were studied using an RNA-seq atlas and qRT-PCR. The majority of RsDofs were tissue-specific and Cd-sensitive. The involvement of RsDof genes in Cd stress response and anthocyanin synthesis was verified using qRT-PCR. RsDof33 is involved in Cd stress response and anthocyanin synthesis. A. thaliana overexpressed the recombinant fusion protein RsDof33-GFP, which was localized to the nucleus, resulting in fewer rosette leaves, delayed flowering, and higher anthocyanin concentration. RsDof33-expressing plants had significantly higher transcript levels of the auxin biosynthetic genes YUCCA (AtYUC2), auxin efflux carrier (AtPIN4), and AtKNAT2, which are involved in leaf shape development, as well as AtPAL, AtCHS, AtCHI, AtDFR, AtLDOX, and AtUF3GT. These findings indicate that RsDofs are critical to plant development and stress responses. |
| format | Article |
| id | doaj-art-250c95414d854a0292fe465cf1fe3636 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-250c95414d854a0292fe465cf1fe36362025-02-09T12:32:58ZengNature PortfolioScientific Reports2045-23222025-02-0115112010.1038/s41598-025-88308-6Genome wide identification of Dof transcription factors in Carmine radish reveals RsDof33 role in cadmium stress and anthocyanin biosynthesisCaiming Gou0Jia Li1Bo Chen2Guoting Cheng3Zhangfei Zheng4Hua Peng5Ahmed H. El-Sappah6School of Agriculture, Forestry and Food Engineering, Yibin UniversitySchool of Agriculture, Forestry and Food Engineering, Yibin UniversitySchool of Agriculture, Forestry and Food Engineering, Yibin UniversityShaanxi Key Laboratory of Research and Utilization of Resource Plants On the Loess Plateau, College of Life Sciences, ‘Yan’an UniversityCollege of Agriculture, Fujian Agriculture and Forestry UniversityResearch Center for Tourism Agriculture Development, Sichuan Tourism CollegeSchool of Agriculture, Forestry and Food Engineering, Yibin UniversityAbstract Carmine radish (Raphanus sativus L.) is cultivated in Fuling, Chongqing, for its red color. Dof-TFs are critical in regulating plant growth, development, stress responses, and signal transduction.This work comprehensively examined the structure, evolution, and expression of the carmine radish Dof gene and its behavior under cadmium (Cd) stress. The radish genome has 59 RsDofs, which are divided into nine clusters (A: 8, B1: 10, B2: 10, C1: 3, C2.1: 5, C2.2: 4, C3: 11, D1: 4, and D2: 4). Phylogenetic tree analysis revealed significant Dof gene family resemblance between Arabidopsis thaliana and Brassica napus. Perhaps segment duplication resulted in RsDof gene family expansion. Cd stress-induced RsDof expression patterns were studied using an RNA-seq atlas and qRT-PCR. The majority of RsDofs were tissue-specific and Cd-sensitive. The involvement of RsDof genes in Cd stress response and anthocyanin synthesis was verified using qRT-PCR. RsDof33 is involved in Cd stress response and anthocyanin synthesis. A. thaliana overexpressed the recombinant fusion protein RsDof33-GFP, which was localized to the nucleus, resulting in fewer rosette leaves, delayed flowering, and higher anthocyanin concentration. RsDof33-expressing plants had significantly higher transcript levels of the auxin biosynthetic genes YUCCA (AtYUC2), auxin efflux carrier (AtPIN4), and AtKNAT2, which are involved in leaf shape development, as well as AtPAL, AtCHS, AtCHI, AtDFR, AtLDOX, and AtUF3GT. These findings indicate that RsDofs are critical to plant development and stress responses.https://doi.org/10.1038/s41598-025-88308-6Raphanus sativus L.Dof transcription factorsAnthocyanin biosynthesisExpression profile: OERsDof33 |
| spellingShingle | Caiming Gou Jia Li Bo Chen Guoting Cheng Zhangfei Zheng Hua Peng Ahmed H. El-Sappah Genome wide identification of Dof transcription factors in Carmine radish reveals RsDof33 role in cadmium stress and anthocyanin biosynthesis Scientific Reports Raphanus sativus L. Dof transcription factors Anthocyanin biosynthesis Expression profile: OERsDof33 |
| title | Genome wide identification of Dof transcription factors in Carmine radish reveals RsDof33 role in cadmium stress and anthocyanin biosynthesis |
| title_full | Genome wide identification of Dof transcription factors in Carmine radish reveals RsDof33 role in cadmium stress and anthocyanin biosynthesis |
| title_fullStr | Genome wide identification of Dof transcription factors in Carmine radish reveals RsDof33 role in cadmium stress and anthocyanin biosynthesis |
| title_full_unstemmed | Genome wide identification of Dof transcription factors in Carmine radish reveals RsDof33 role in cadmium stress and anthocyanin biosynthesis |
| title_short | Genome wide identification of Dof transcription factors in Carmine radish reveals RsDof33 role in cadmium stress and anthocyanin biosynthesis |
| title_sort | genome wide identification of dof transcription factors in carmine radish reveals rsdof33 role in cadmium stress and anthocyanin biosynthesis |
| topic | Raphanus sativus L. Dof transcription factors Anthocyanin biosynthesis Expression profile: OERsDof33 |
| url | https://doi.org/10.1038/s41598-025-88308-6 |
| work_keys_str_mv | AT caiminggou genomewideidentificationofdoftranscriptionfactorsincarmineradishrevealsrsdof33roleincadmiumstressandanthocyaninbiosynthesis AT jiali genomewideidentificationofdoftranscriptionfactorsincarmineradishrevealsrsdof33roleincadmiumstressandanthocyaninbiosynthesis AT bochen genomewideidentificationofdoftranscriptionfactorsincarmineradishrevealsrsdof33roleincadmiumstressandanthocyaninbiosynthesis AT guotingcheng genomewideidentificationofdoftranscriptionfactorsincarmineradishrevealsrsdof33roleincadmiumstressandanthocyaninbiosynthesis AT zhangfeizheng genomewideidentificationofdoftranscriptionfactorsincarmineradishrevealsrsdof33roleincadmiumstressandanthocyaninbiosynthesis AT huapeng genomewideidentificationofdoftranscriptionfactorsincarmineradishrevealsrsdof33roleincadmiumstressandanthocyaninbiosynthesis AT ahmedhelsappah genomewideidentificationofdoftranscriptionfactorsincarmineradishrevealsrsdof33roleincadmiumstressandanthocyaninbiosynthesis |