Optimization of extraction and enrichment process of cannabidiol from industrial hemp and evaluation of its bioactivity
IntroductionThe Cannabis Sativa L., a perennial dioecious herb renowned for its industrial applications, serves as the source of hemp. Cannabidiol (CBD), a non-psychotropic compound derived from industrial hemp, has garnered considerable interest due to its promising therapeutic potential.MethodsThe...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2025.1495779/full |
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| author | Junkai Wu Xiaomeng Zhang Xiaoqing Liu Zunlai Sheng Zunlai Sheng Jianping Hu Feiyan Zhang |
| author_facet | Junkai Wu Xiaomeng Zhang Xiaoqing Liu Zunlai Sheng Zunlai Sheng Jianping Hu Feiyan Zhang |
| author_sort | Junkai Wu |
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| description | IntroductionThe Cannabis Sativa L., a perennial dioecious herb renowned for its industrial applications, serves as the source of hemp. Cannabidiol (CBD), a non-psychotropic compound derived from industrial hemp, has garnered considerable interest due to its promising therapeutic potential.MethodsThe extraction parameters for CBD from industrial hemp were optimized using the Box-Behnken design and response surface methodology (RSM). The purification process involved characterizing the penetration and desorption profiles of CBD on HPD-100 resin. The in vitro antibacterial activity was assessed by determining the minimum inhibitory concentration (MIC) against Staphylococcus aureus and Escherichia coli. Antioxidant properties were evaluated using DPPH and ABTS assays, as well as an iron-reducing ability test.ResultsAfter optimization, the extraction rate of CBD reached 0.26 ± 0.02%. The use of HP-100 resin in the purification process resulted in a significant enrichment of CBD content, which was 4.2 times higher than that of the crude extract, with a recovery rate of 83.13%. The MIC against S. aureus was found to be 5 mg/mL, while no inhibitory effect was observed against E. coli. The IC50 values for the DPPH and ABTS assays were 0.1875 mg/mL and 2.988 mg/mL, respectively, indicating the potent antioxidant capacity of CBD. Additionally, CBD demonstrated a strong iron-reducing ability.ConclusionThese findings contribute to the development of CBD for broader applications in various industries, highlighting its potential as a valuable compound in health and wellness sectors. |
| format | Article |
| id | doaj-art-26d6b1767a0f413ea3240f287f90a760 |
| institution | Kabale University |
| issn | 1664-462X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Plant Science |
| spelling | doaj-art-26d6b1767a0f413ea3240f287f90a7602025-02-07T06:49:40ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2025-02-011610.3389/fpls.2025.14957791495779Optimization of extraction and enrichment process of cannabidiol from industrial hemp and evaluation of its bioactivityJunkai Wu0Xiaomeng Zhang1Xiaoqing Liu2Zunlai Sheng3Zunlai Sheng4Jianping Hu5Feiyan Zhang6School of Pharmacy, Quanzhou Medical College, Quanzhou, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaCollege of Veterinary Medicine, Northeast Agricultural University, Harbin, ChinaHeilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, Northeast Agricultural University, Harbin, ChinaSchool of Pharmacy, Quanzhou Medical College, Quanzhou, ChinaSchool of Pharmacy, Quanzhou Medical College, Quanzhou, ChinaIntroductionThe Cannabis Sativa L., a perennial dioecious herb renowned for its industrial applications, serves as the source of hemp. Cannabidiol (CBD), a non-psychotropic compound derived from industrial hemp, has garnered considerable interest due to its promising therapeutic potential.MethodsThe extraction parameters for CBD from industrial hemp were optimized using the Box-Behnken design and response surface methodology (RSM). The purification process involved characterizing the penetration and desorption profiles of CBD on HPD-100 resin. The in vitro antibacterial activity was assessed by determining the minimum inhibitory concentration (MIC) against Staphylococcus aureus and Escherichia coli. Antioxidant properties were evaluated using DPPH and ABTS assays, as well as an iron-reducing ability test.ResultsAfter optimization, the extraction rate of CBD reached 0.26 ± 0.02%. The use of HP-100 resin in the purification process resulted in a significant enrichment of CBD content, which was 4.2 times higher than that of the crude extract, with a recovery rate of 83.13%. The MIC against S. aureus was found to be 5 mg/mL, while no inhibitory effect was observed against E. coli. The IC50 values for the DPPH and ABTS assays were 0.1875 mg/mL and 2.988 mg/mL, respectively, indicating the potent antioxidant capacity of CBD. Additionally, CBD demonstrated a strong iron-reducing ability.ConclusionThese findings contribute to the development of CBD for broader applications in various industries, highlighting its potential as a valuable compound in health and wellness sectors.https://www.frontiersin.org/articles/10.3389/fpls.2025.1495779/fullindustrial hempcannabidiolextraction processenrichment processbioactivity evaluation |
| spellingShingle | Junkai Wu Xiaomeng Zhang Xiaoqing Liu Zunlai Sheng Zunlai Sheng Jianping Hu Feiyan Zhang Optimization of extraction and enrichment process of cannabidiol from industrial hemp and evaluation of its bioactivity Frontiers in Plant Science industrial hemp cannabidiol extraction process enrichment process bioactivity evaluation |
| title | Optimization of extraction and enrichment process of cannabidiol from industrial hemp and evaluation of its bioactivity |
| title_full | Optimization of extraction and enrichment process of cannabidiol from industrial hemp and evaluation of its bioactivity |
| title_fullStr | Optimization of extraction and enrichment process of cannabidiol from industrial hemp and evaluation of its bioactivity |
| title_full_unstemmed | Optimization of extraction and enrichment process of cannabidiol from industrial hemp and evaluation of its bioactivity |
| title_short | Optimization of extraction and enrichment process of cannabidiol from industrial hemp and evaluation of its bioactivity |
| title_sort | optimization of extraction and enrichment process of cannabidiol from industrial hemp and evaluation of its bioactivity |
| topic | industrial hemp cannabidiol extraction process enrichment process bioactivity evaluation |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2025.1495779/full |
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