Explication of the mixture of <i>Piper betle</i> and <i>Eugenia polyantha</i> on oral bacteria
The aim of this study was to analyze the effectiveness of the mixtures of Eugenia polyantha (EP) and Piper betle (PB) leaves against oral bacteria. Four compositions of EP and PB were made and observed on tryptic soy agar (TSA), blood agar (BA), MacConkey agar (MCA), and mannitol salt agar (MSA). I...
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PAGEPress Publications
2025-02-01
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| Series: | Healthcare in Low-resource Settings |
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| Online Access: | https://www.pagepressjournals.org/hls/article/view/13016 |
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| author | Neneng Nurjanah Megananda Hiranya Putri Yenni Hendriani Praptiwi Dewi Sodja Laela Susi Sukmasari |
| author_facet | Neneng Nurjanah Megananda Hiranya Putri Yenni Hendriani Praptiwi Dewi Sodja Laela Susi Sukmasari |
| author_sort | Neneng Nurjanah |
| collection | DOAJ |
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The aim of this study was to analyze the effectiveness of the mixtures of Eugenia polyantha (EP) and Piper betle (PB) leaves against oral bacteria. Four compositions of EP and PB were made and observed on tryptic soy agar (TSA), blood agar (BA), MacConkey agar (MCA), and mannitol salt agar (MSA). Inhibition analysis was conducted at concentrations of 20%, 30%, 40%, 50%, and 60%. Our study results indicate that Staphylococcus aureus exhibited growth on BA, bacterial colonies were identified on MSA, and serological tests confirmed coagulation. A biochemical test conducted on an MCA sample revealed several species within the Enterobacteriaceae family, including Escherichia coli, Pseudomonas, Klebsiella, and Proteus. The highest level of inhibition was observed with the sixth toothbrush (K4). Significant differences in inhibition were noted among the various groups (K1, K2, K3, K4, and controls), with the diameter of inhibition for each combination yielding p<0.05. However, the differences in inhibition between the combinations themselves were not statistically significant, with p>0.05. Streptococcus sp, Klebsiella sp, Enterobacteriaceae sp (Coliform sp), Pseudomonas, and Staphylococcus aureus are the contaminant bacteria on used toothbrushes. The highest inhibition level and bactericidal properties were achieved by mixing 70% bay leaf (Eugenia polyantha) and 30% betel leaf infusion.
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| format | Article |
| id | doaj-art-292a4871f30f49438249edeaa18d71a9 |
| institution | Kabale University |
| issn | 2281-7824 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | Healthcare in Low-resource Settings |
| spelling | doaj-art-292a4871f30f49438249edeaa18d71a92025-02-11T10:56:40ZengPAGEPress PublicationsHealthcare in Low-resource Settings2281-78242025-02-0110.4081/hls.2025.13016Explication of the mixture of <i>Piper betle</i> and <i>Eugenia polyantha</i> on oral bacteriaNeneng Nurjanah0Megananda Hiranya Putri1Yenni Hendriani Praptiwi2Dewi Sodja Laela3Susi Sukmasari4Department of Dental Nurse, Bandung Polytechnic of Health, Jawa BaratDepartment of Dental Nurse, Bandung Polytechnic of Health, Jawa BaratDepartment of Dental Nurse, Bandung Polytechnic of Health, Jawa BaratDepartment of Dental Nurse, Bandung Polytechnic of Health, Jawa BaratDepartment of Paediatric Dentistry and Dental Public Health, Kulliyyah of Dentistry, International Islamic University Malaysia, Kuantan, Pahang The aim of this study was to analyze the effectiveness of the mixtures of Eugenia polyantha (EP) and Piper betle (PB) leaves against oral bacteria. Four compositions of EP and PB were made and observed on tryptic soy agar (TSA), blood agar (BA), MacConkey agar (MCA), and mannitol salt agar (MSA). Inhibition analysis was conducted at concentrations of 20%, 30%, 40%, 50%, and 60%. Our study results indicate that Staphylococcus aureus exhibited growth on BA, bacterial colonies were identified on MSA, and serological tests confirmed coagulation. A biochemical test conducted on an MCA sample revealed several species within the Enterobacteriaceae family, including Escherichia coli, Pseudomonas, Klebsiella, and Proteus. The highest level of inhibition was observed with the sixth toothbrush (K4). Significant differences in inhibition were noted among the various groups (K1, K2, K3, K4, and controls), with the diameter of inhibition for each combination yielding p<0.05. However, the differences in inhibition between the combinations themselves were not statistically significant, with p>0.05. Streptococcus sp, Klebsiella sp, Enterobacteriaceae sp (Coliform sp), Pseudomonas, and Staphylococcus aureus are the contaminant bacteria on used toothbrushes. The highest inhibition level and bactericidal properties were achieved by mixing 70% bay leaf (Eugenia polyantha) and 30% betel leaf infusion. https://www.pagepressjournals.org/hls/article/view/13016Piper betleEugenia polyanthanatural disinfectionused toothbrushes |
| spellingShingle | Neneng Nurjanah Megananda Hiranya Putri Yenni Hendriani Praptiwi Dewi Sodja Laela Susi Sukmasari Explication of the mixture of <i>Piper betle</i> and <i>Eugenia polyantha</i> on oral bacteria Healthcare in Low-resource Settings Piper betle Eugenia polyantha natural disinfection used toothbrushes |
| title | Explication of the mixture of <i>Piper betle</i> and <i>Eugenia polyantha</i> on oral bacteria |
| title_full | Explication of the mixture of <i>Piper betle</i> and <i>Eugenia polyantha</i> on oral bacteria |
| title_fullStr | Explication of the mixture of <i>Piper betle</i> and <i>Eugenia polyantha</i> on oral bacteria |
| title_full_unstemmed | Explication of the mixture of <i>Piper betle</i> and <i>Eugenia polyantha</i> on oral bacteria |
| title_short | Explication of the mixture of <i>Piper betle</i> and <i>Eugenia polyantha</i> on oral bacteria |
| title_sort | explication of the mixture of i piper betle i and i eugenia polyantha i on oral bacteria |
| topic | Piper betle Eugenia polyantha natural disinfection used toothbrushes |
| url | https://www.pagepressjournals.org/hls/article/view/13016 |
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