Confocal Live Imaging of Reproductive Organs Development in Arabidopsis
Understanding how multicellular organisms are shaped requires high-resolution, quantitative data to unravel how biological structures grow and develop over time. In recent years, confocal live imaging has become an essential tool providing insights into developmental dynamics at cellular resolution...
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| Format: | Article |
| Language: | English |
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Bio-protocol LLC
2025-02-01
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| Series: | Bio-Protocol |
| Online Access: | https://bio-protocol.org/en/bpdetail?id=5177&type=0 |
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| author | Binghan Wang Amélie Bauer Andrea Gómez-Felipe Sylvia Silveira Daniel Kierzkowski |
| author_facet | Binghan Wang Amélie Bauer Andrea Gómez-Felipe Sylvia Silveira Daniel Kierzkowski |
| author_sort | Binghan Wang |
| collection | DOAJ |
| description | Understanding how multicellular organisms are shaped requires high-resolution, quantitative data to unravel how biological structures grow and develop over time. In recent years, confocal live imaging has become an essential tool providing insights into developmental dynamics at cellular resolution in plant organs such as leaves or meristems. In the context of flowers, growth tracking has primarily been limited to sepals, the outermost floral organs, or the post-fertilization gynoecium, which are easily accessible for microscopy. Here, we describe a detailed pipeline for the preparation, dissection, and confocal imaging of the development of internal reproductive floral organs of Arabidopsis thaliana including both the stamen and gynoecium. We also discuss how to acquire high-quality images suitable for efficient 2D and 3D segmentation that allow the quantification of cellular dynamics underlying their development. |
| format | Article |
| id | doaj-art-2a396e12b9f248858e9a0bf8d20768e6 |
| institution | Kabale University |
| issn | 2331-8325 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Bio-protocol LLC |
| record_format | Article |
| series | Bio-Protocol |
| spelling | doaj-art-2a396e12b9f248858e9a0bf8d20768e62025-02-07T08:16:46ZengBio-protocol LLCBio-Protocol2331-83252025-02-0115310.21769/BioProtoc.5177Confocal Live Imaging of Reproductive Organs Development in ArabidopsisBinghan Wang0Amélie Bauer1Andrea Gómez-Felipe2Sylvia Silveira3Daniel Kierzkowski4Institut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, 4101 Sherbrooke St E, Montréal, QC, CanadaInstitut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, 4101 Sherbrooke St E, Montréal, QC, CanadaInstitut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, 4101 Sherbrooke St E, Montréal, QC, CanadaInstitut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, 4101 Sherbrooke St E, Montréal, QC, CanadaInstitut de Recherche en Biologie Végétale, Département de Sciences Biologiques, Université de Montréal, 4101 Sherbrooke St E, Montréal, QC, CanadaUnderstanding how multicellular organisms are shaped requires high-resolution, quantitative data to unravel how biological structures grow and develop over time. In recent years, confocal live imaging has become an essential tool providing insights into developmental dynamics at cellular resolution in plant organs such as leaves or meristems. In the context of flowers, growth tracking has primarily been limited to sepals, the outermost floral organs, or the post-fertilization gynoecium, which are easily accessible for microscopy. Here, we describe a detailed pipeline for the preparation, dissection, and confocal imaging of the development of internal reproductive floral organs of Arabidopsis thaliana including both the stamen and gynoecium. We also discuss how to acquire high-quality images suitable for efficient 2D and 3D segmentation that allow the quantification of cellular dynamics underlying their development.https://bio-protocol.org/en/bpdetail?id=5177&type=0 |
| spellingShingle | Binghan Wang Amélie Bauer Andrea Gómez-Felipe Sylvia Silveira Daniel Kierzkowski Confocal Live Imaging of Reproductive Organs Development in Arabidopsis Bio-Protocol |
| title | Confocal Live Imaging of Reproductive Organs Development in Arabidopsis |
| title_full | Confocal Live Imaging of Reproductive Organs Development in Arabidopsis |
| title_fullStr | Confocal Live Imaging of Reproductive Organs Development in Arabidopsis |
| title_full_unstemmed | Confocal Live Imaging of Reproductive Organs Development in Arabidopsis |
| title_short | Confocal Live Imaging of Reproductive Organs Development in Arabidopsis |
| title_sort | confocal live imaging of reproductive organs development in arabidopsis |
| url | https://bio-protocol.org/en/bpdetail?id=5177&type=0 |
| work_keys_str_mv | AT binghanwang confocalliveimagingofreproductiveorgansdevelopmentinarabidopsis AT ameliebauer confocalliveimagingofreproductiveorgansdevelopmentinarabidopsis AT andreagomezfelipe confocalliveimagingofreproductiveorgansdevelopmentinarabidopsis AT sylviasilveira confocalliveimagingofreproductiveorgansdevelopmentinarabidopsis AT danielkierzkowski confocalliveimagingofreproductiveorgansdevelopmentinarabidopsis |