Development of an innovative intravaginal model of probiotic inoculation in dairy ovine flocks: positive effects on the vaginal microbiota, vaginitis and fertility
In vitro studies have reported the antimicrobial effect of an inoculum (L2) made from commercial probiotic strains of Lactobacillus spp. (Lactobacillus (L.) crispatus, L. brevis and L. gasseri) against some ruminants’ pathogens. However, their possible beneficial effects on controlling the vaginosis...
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Elsevier
2025-02-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1751731124003549 |
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| author | M. Toquet J. Gomis E. Jiménez-Trigos E. Bataller M. Barba A. Sánchez P. González-Torres Á. Gómez-Martín |
| author_facet | M. Toquet J. Gomis E. Jiménez-Trigos E. Bataller M. Barba A. Sánchez P. González-Torres Á. Gómez-Martín |
| author_sort | M. Toquet |
| collection | DOAJ |
| description | In vitro studies have reported the antimicrobial effect of an inoculum (L2) made from commercial probiotic strains of Lactobacillus spp. (Lactobacillus (L.) crispatus, L. brevis and L. gasseri) against some ruminants’ pathogens. However, their possible beneficial effects on controlling the vaginosis caused by the use of intravaginal sponges for oestrus synchronisation have not been evaluated in in vivo models. Therefore, the purpose of this study was to perform an intravaginal double inoculation of this same non-lyophilised (non-freeze-dried) L2 inoculum, in 120 ewes from four dairy commercial ovine flocks using straws similar to those used in artificial insemination. Globally, in the entire study population, the fertility percentage (65%) of the inoculated groups increased by 10% compared to the control groups (55%). Specifically, increases in the percentages of pregnant sheep in flocks 1 and 4 (10.7 and 5.7% respectively; P > 0.05) and flock 3 (27.3%; P < 0.05) were observed in inoculated ewes. Overall, the administration of L2 on the day of intravaginal sponge insertion decreased the number of ewes with vaginitis by 11.13% at the time of sponge removal (T1). In the first flock, the administration of the probiotic positively modulated the microbiota with an increased richness and presence of Lactobacillales accompanied by a reduction in Mycoplasma spp. Genera such as Fusobacterium, Streptobacillus, Campylobacter or Trueperella were linked to the presence of vaginitis at T1. Genera belonging to Lactobacillales, including some genera previously reported to be beneficial for pregnancy, significantly increased at the time of pregnancy diagnosis but significantly decreased at T1. The results of this study suggest that the use of probiotics via intravaginal inoculation as a strategy to positively modulate the microbiota and vaginal inflammation, derived from the use of intravaginal sponges, is also associated with improved fertility. This novel approach could improve animal welfare in oestrus-synchronisation protocols and represents an alternative to the prophylactic use of antibiotics. |
| format | Article |
| id | doaj-art-2a61da88b40e496287072d571798e1e1 |
| institution | Kabale University |
| issn | 1751-7311 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
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| series | Animal |
| spelling | doaj-art-2a61da88b40e496287072d571798e1e12025-02-12T05:30:55ZengElsevierAnimal1751-73112025-02-01192101417Development of an innovative intravaginal model of probiotic inoculation in dairy ovine flocks: positive effects on the vaginal microbiota, vaginitis and fertilityM. Toquet0J. Gomis1E. Jiménez-Trigos2E. Bataller3M. Barba4A. Sánchez5P. González-Torres6Á. Gómez-Martín7Microbiological Agents Associated with Animal Reproduction (ProVaginBIO) Research Group, Departamento Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera‑CEU, CEU Universities, Carrer Tirant lo Blanc, 7, 46115 Alfara del Patriarca, Valencia, SpainMicrobiological Agents Associated with Animal Reproduction (ProVaginBIO) Research Group, Departamento Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera‑CEU, CEU Universities, Carrer Tirant lo Blanc, 7, 46115 Alfara del Patriarca, Valencia, SpainMicrobiological Agents Associated with Animal Reproduction (ProVaginBIO) Research Group, Departamento Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera‑CEU, CEU Universities, Carrer Tirant lo Blanc, 7, 46115 Alfara del Patriarca, Valencia, SpainMicrobiological Agents Associated with Animal Reproduction (ProVaginBIO) Research Group, Departamento Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera‑CEU, CEU Universities, Carrer Tirant lo Blanc, 7, 46115 Alfara del Patriarca, Valencia, SpainAgrifood Research and Technology Centre of Aragon (CITA), Calle Corinto 3, 44159, Teruel, SpainRuminant Health Research Group, Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Murcia, Campus de Espinardo, 30100 Murcia, SpainMicrobiological Agents Associated with Animal Reproduction (ProVaginBIO) Research Group, Departamento Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera‑CEU, CEU Universities, Carrer Tirant lo Blanc, 7, 46115 Alfara del Patriarca, Valencia, Spain; Microomics Systems S.L., Barcelona, SpainMicrobiological Agents Associated with Animal Reproduction (ProVaginBIO) Research Group, Departamento Producción y Sanidad Animal, Salud Pública Veterinaria y Ciencia y Tecnología de los Alimentos, Facultad de Veterinaria, Universidad Cardenal Herrera‑CEU, CEU Universities, Carrer Tirant lo Blanc, 7, 46115 Alfara del Patriarca, Valencia, Spain; Corresponding author.In vitro studies have reported the antimicrobial effect of an inoculum (L2) made from commercial probiotic strains of Lactobacillus spp. (Lactobacillus (L.) crispatus, L. brevis and L. gasseri) against some ruminants’ pathogens. However, their possible beneficial effects on controlling the vaginosis caused by the use of intravaginal sponges for oestrus synchronisation have not been evaluated in in vivo models. Therefore, the purpose of this study was to perform an intravaginal double inoculation of this same non-lyophilised (non-freeze-dried) L2 inoculum, in 120 ewes from four dairy commercial ovine flocks using straws similar to those used in artificial insemination. Globally, in the entire study population, the fertility percentage (65%) of the inoculated groups increased by 10% compared to the control groups (55%). Specifically, increases in the percentages of pregnant sheep in flocks 1 and 4 (10.7 and 5.7% respectively; P > 0.05) and flock 3 (27.3%; P < 0.05) were observed in inoculated ewes. Overall, the administration of L2 on the day of intravaginal sponge insertion decreased the number of ewes with vaginitis by 11.13% at the time of sponge removal (T1). In the first flock, the administration of the probiotic positively modulated the microbiota with an increased richness and presence of Lactobacillales accompanied by a reduction in Mycoplasma spp. Genera such as Fusobacterium, Streptobacillus, Campylobacter or Trueperella were linked to the presence of vaginitis at T1. Genera belonging to Lactobacillales, including some genera previously reported to be beneficial for pregnancy, significantly increased at the time of pregnancy diagnosis but significantly decreased at T1. The results of this study suggest that the use of probiotics via intravaginal inoculation as a strategy to positively modulate the microbiota and vaginal inflammation, derived from the use of intravaginal sponges, is also associated with improved fertility. This novel approach could improve animal welfare in oestrus-synchronisation protocols and represents an alternative to the prophylactic use of antibiotics.http://www.sciencedirect.com/science/article/pii/S1751731124003549Alternative to antibiotic therapyAnimal welfareEwesLactobacillusMollicutes |
| spellingShingle | M. Toquet J. Gomis E. Jiménez-Trigos E. Bataller M. Barba A. Sánchez P. González-Torres Á. Gómez-Martín Development of an innovative intravaginal model of probiotic inoculation in dairy ovine flocks: positive effects on the vaginal microbiota, vaginitis and fertility Animal Alternative to antibiotic therapy Animal welfare Ewes Lactobacillus Mollicutes |
| title | Development of an innovative intravaginal model of probiotic inoculation in dairy ovine flocks: positive effects on the vaginal microbiota, vaginitis and fertility |
| title_full | Development of an innovative intravaginal model of probiotic inoculation in dairy ovine flocks: positive effects on the vaginal microbiota, vaginitis and fertility |
| title_fullStr | Development of an innovative intravaginal model of probiotic inoculation in dairy ovine flocks: positive effects on the vaginal microbiota, vaginitis and fertility |
| title_full_unstemmed | Development of an innovative intravaginal model of probiotic inoculation in dairy ovine flocks: positive effects on the vaginal microbiota, vaginitis and fertility |
| title_short | Development of an innovative intravaginal model of probiotic inoculation in dairy ovine flocks: positive effects on the vaginal microbiota, vaginitis and fertility |
| title_sort | development of an innovative intravaginal model of probiotic inoculation in dairy ovine flocks positive effects on the vaginal microbiota vaginitis and fertility |
| topic | Alternative to antibiotic therapy Animal welfare Ewes Lactobacillus Mollicutes |
| url | http://www.sciencedirect.com/science/article/pii/S1751731124003549 |
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