Molecular genetic and bacteriological methods of bovine mycoplasmosis diagnosis
Mycoplasmas are bacteria that are extremely unstable in vitro as they lack a rigid cell wall. They are most often detected in association with other pathogens, including those that can become L-forms if treated with antibiotics. Mycoplasma colonies, as well as colonies of L-form bacteria, have a typ...
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Da Vinci Media
2022-12-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/678 |
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| author | E. V. Remizova A. V. Gorbatov L. K. Semina Z. A. Skulyabina N. V. Shmidt G. A. Baldicheva N. N. Avduevskaya |
| author_facet | E. V. Remizova A. V. Gorbatov L. K. Semina Z. A. Skulyabina N. V. Shmidt G. A. Baldicheva N. N. Avduevskaya |
| author_sort | E. V. Remizova |
| collection | DOAJ |
| description | Mycoplasmas are bacteria that are extremely unstable in vitro as they lack a rigid cell wall. They are most often detected in association with other pathogens, including those that can become L-forms if treated with antibiotics. Mycoplasma colonies, as well as colonies of L-form bacteria, have a typical «fried egg» appearance, therefore it is necessary to differentiate them for the accurate diagnosis and choice of treatment. The paper presents data on mycoplasma infection diagnosis in cattle and results of differentiation of isolated mycoplasma and L-form bacteria colonies using multiple passaging and real-time polymerase chain reaction. For that, 177 samples were collected from animals with mycoplasmosis clinical signs, 45 of them were tested using molecular genetic method, 132 samples were subjected to bacteriological testing. Mycoplasma DNA was detected in 71.1% of samples, and specific colonies were detected in 3.8% of samples. Such biochemical tests of mycoplasma species identification as arginine hydrolysis, blood serum liquefaction, film and grain formation, inoculation into Tween-80-containing medium, hemadsorption and hemolysis of erythrocytes do not allow an objective assessment of the species belonging to mycoplasmas, but, according to the results obtained, the isolated species most likely belongs to Mycoplasma dispar, which is pathogenic for cattle. Real-time polymerase chain reaction is undoubtedly the most accurate and rapid diagnostic method for mycoplasmosis, but a preliminary diagnosis can also be established bacteriologically within 2–7 days. In addition, during microbiological testing, it is possible to assess the antibiotic resistance of mycoplasma isolates, thereby developing an optimal and high-quality scheme of the disease treatment and prevention. |
| format | Article |
| id | doaj-art-2c50604d0ca64601b15bc03653587212 |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2022-12-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-2c50604d0ca64601b15bc036535872122025-02-06T09:52:09ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592022-12-0111433534010.29326/2304-196X-2022-11-4-335-340589Molecular genetic and bacteriological methods of bovine mycoplasmosis diagnosisE. V. Remizova0A. V. Gorbatov1L. K. Semina2Z. A. Skulyabina3N. V. Shmidt4G. A. Baldicheva5N. N. Avduevskaya6Vologda Branch of the Federal State Budget Scientific Institution «Federal Scientific Centre VIEV» (Vologda Branch of the FSC VIEV)Federal State Budget Scientific Institution «Federal Scientific Centre VIEV» (FSC VIEV)Vologda Branch of the Federal State Budget Scientific Institution «Federal Scientific Centre VIEV» (Vologda Branch of the FSC VIEV)Vologda Branch of the Federal State Budget Scientific Institution «Federal Scientific Centre VIEV» (Vologda Branch of the FSC VIEV)State-Financed Veterinary Institution of the Vologda Oblast «Vologda Oblast Veterinary Laboratory» (SFVIVO «Vologda OVL»)Vologda Branch of the Federal State Budget Scientific Institution «Federal Scientific Centre VIEV» (Vologda Branch of the FSC VIEV)Vologda Branch of the Federal State Budget Scientific Institution «Federal Scientific Centre VIEV» (Vologda Branch of the FSC VIEV)Mycoplasmas are bacteria that are extremely unstable in vitro as they lack a rigid cell wall. They are most often detected in association with other pathogens, including those that can become L-forms if treated with antibiotics. Mycoplasma colonies, as well as colonies of L-form bacteria, have a typical «fried egg» appearance, therefore it is necessary to differentiate them for the accurate diagnosis and choice of treatment. The paper presents data on mycoplasma infection diagnosis in cattle and results of differentiation of isolated mycoplasma and L-form bacteria colonies using multiple passaging and real-time polymerase chain reaction. For that, 177 samples were collected from animals with mycoplasmosis clinical signs, 45 of them were tested using molecular genetic method, 132 samples were subjected to bacteriological testing. Mycoplasma DNA was detected in 71.1% of samples, and specific colonies were detected in 3.8% of samples. Such biochemical tests of mycoplasma species identification as arginine hydrolysis, blood serum liquefaction, film and grain formation, inoculation into Tween-80-containing medium, hemadsorption and hemolysis of erythrocytes do not allow an objective assessment of the species belonging to mycoplasmas, but, according to the results obtained, the isolated species most likely belongs to Mycoplasma dispar, which is pathogenic for cattle. Real-time polymerase chain reaction is undoubtedly the most accurate and rapid diagnostic method for mycoplasmosis, but a preliminary diagnosis can also be established bacteriologically within 2–7 days. In addition, during microbiological testing, it is possible to assess the antibiotic resistance of mycoplasma isolates, thereby developing an optimal and high-quality scheme of the disease treatment and prevention.https://veterinary.arriah.ru/jour/article/view/678mycoplasmabacteriological diagnosisfactor infectionmicroflorapneumonial-form bacteriaspecific nutrient mediamolecular genetic diagnosis |
| spellingShingle | E. V. Remizova A. V. Gorbatov L. K. Semina Z. A. Skulyabina N. V. Shmidt G. A. Baldicheva N. N. Avduevskaya Molecular genetic and bacteriological methods of bovine mycoplasmosis diagnosis Ветеринария сегодня mycoplasma bacteriological diagnosis factor infection microflora pneumonia l-form bacteria specific nutrient media molecular genetic diagnosis |
| title | Molecular genetic and bacteriological methods of bovine mycoplasmosis diagnosis |
| title_full | Molecular genetic and bacteriological methods of bovine mycoplasmosis diagnosis |
| title_fullStr | Molecular genetic and bacteriological methods of bovine mycoplasmosis diagnosis |
| title_full_unstemmed | Molecular genetic and bacteriological methods of bovine mycoplasmosis diagnosis |
| title_short | Molecular genetic and bacteriological methods of bovine mycoplasmosis diagnosis |
| title_sort | molecular genetic and bacteriological methods of bovine mycoplasmosis diagnosis |
| topic | mycoplasma bacteriological diagnosis factor infection microflora pneumonia l-form bacteria specific nutrient media molecular genetic diagnosis |
| url | https://veterinary.arriah.ru/jour/article/view/678 |
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