Genomic characterization of foodborne Salmonella enterica and Escherichia coli isolates from Saboba district and Bolgatanga Municipality Ghana.
Salmonella enterica and Escherichia coli are well-known bacteria commonly associated with foodborne illnesses in humans and animals. Genomic characterization of these pathogens provides valuable insights into their evolution, virulence factors and resistance determinants. This study aimed to charact...
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Public Library of Science (PLoS)
2025-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0315583 |
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| author | Gabriel Temitope Sunmonu Frederick Adzitey Erkison Ewomazino Odih Boniface Awini Tibile Rejoice Ekli Martin Aduah Anderson O Oaikhena Olabisi C Akinlabi Akebe Luther King Abia Daniel Gyamfi Amoako Iruka N Okeke |
| author_facet | Gabriel Temitope Sunmonu Frederick Adzitey Erkison Ewomazino Odih Boniface Awini Tibile Rejoice Ekli Martin Aduah Anderson O Oaikhena Olabisi C Akinlabi Akebe Luther King Abia Daniel Gyamfi Amoako Iruka N Okeke |
| author_sort | Gabriel Temitope Sunmonu |
| collection | DOAJ |
| description | Salmonella enterica and Escherichia coli are well-known bacteria commonly associated with foodborne illnesses in humans and animals. Genomic characterization of these pathogens provides valuable insights into their evolution, virulence factors and resistance determinants. This study aimed to characterized previously isolated Salmonella (n = 14) and E. coli (n = 19) from milk, meat and its associated utensils in Ghana using whole-genome sequencing. Most of the Salmonella serovars (Fresno, Plymouth, Infantis, Give and Orleans) identified in this study are yet to be reported in Ghana. Most Salmonella isolates were pan-sensitive, but genes conferring resistance to fosfomycin (fosA7.2) and tetracycline (tet(A)) were detected in one and three isolates, respectively. Seven of the Salmonella isolates carried the IncI1-I(Gamma) plasmid replicon. Although antimicrobial resistance was not common among Salmonella strains, most (11/19) of the E. coli strains had at least one resistance gene, with nearly half (8/19) being multidrug resistant and carried plasmids. Three of the 19 E. coli strains belonged to serovars commonly associated with enteroaggregative E. coli (EAEC) pathotype. While strains belonging to virulence-associated lineages lacked key plasmid-encoded virulence plasmids, several plasmid replicons were detected in most of the E. coli (14/19) strains. Food contaminated with these pathogens can serve as a vehicle for disease transmission, posing a significant public health risk and necessitating stringent food safety and hygiene practices to prevent outbreaks. Hence, there is need for continuous surveillance and preventive measures to stop the spread of foodborne pathogens and reduce the risk of associated illnesses in Ghana. |
| format | Article |
| id | doaj-art-2f0eeb2c79eb4a39ace2ae8c6eb262a1 |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-2f0eeb2c79eb4a39ace2ae8c6eb262a12025-02-12T05:31:12ZengPublic Library of Science (PLoS)PLoS ONE1932-62032025-01-01202e031558310.1371/journal.pone.0315583Genomic characterization of foodborne Salmonella enterica and Escherichia coli isolates from Saboba district and Bolgatanga Municipality Ghana.Gabriel Temitope SunmonuFrederick AdziteyErkison Ewomazino OdihBoniface Awini TibileRejoice EkliMartin AduahAnderson O OaikhenaOlabisi C AkinlabiAkebe Luther King AbiaDaniel Gyamfi AmoakoIruka N OkekeSalmonella enterica and Escherichia coli are well-known bacteria commonly associated with foodborne illnesses in humans and animals. Genomic characterization of these pathogens provides valuable insights into their evolution, virulence factors and resistance determinants. This study aimed to characterized previously isolated Salmonella (n = 14) and E. coli (n = 19) from milk, meat and its associated utensils in Ghana using whole-genome sequencing. Most of the Salmonella serovars (Fresno, Plymouth, Infantis, Give and Orleans) identified in this study are yet to be reported in Ghana. Most Salmonella isolates were pan-sensitive, but genes conferring resistance to fosfomycin (fosA7.2) and tetracycline (tet(A)) were detected in one and three isolates, respectively. Seven of the Salmonella isolates carried the IncI1-I(Gamma) plasmid replicon. Although antimicrobial resistance was not common among Salmonella strains, most (11/19) of the E. coli strains had at least one resistance gene, with nearly half (8/19) being multidrug resistant and carried plasmids. Three of the 19 E. coli strains belonged to serovars commonly associated with enteroaggregative E. coli (EAEC) pathotype. While strains belonging to virulence-associated lineages lacked key plasmid-encoded virulence plasmids, several plasmid replicons were detected in most of the E. coli (14/19) strains. Food contaminated with these pathogens can serve as a vehicle for disease transmission, posing a significant public health risk and necessitating stringent food safety and hygiene practices to prevent outbreaks. Hence, there is need for continuous surveillance and preventive measures to stop the spread of foodborne pathogens and reduce the risk of associated illnesses in Ghana.https://doi.org/10.1371/journal.pone.0315583 |
| spellingShingle | Gabriel Temitope Sunmonu Frederick Adzitey Erkison Ewomazino Odih Boniface Awini Tibile Rejoice Ekli Martin Aduah Anderson O Oaikhena Olabisi C Akinlabi Akebe Luther King Abia Daniel Gyamfi Amoako Iruka N Okeke Genomic characterization of foodborne Salmonella enterica and Escherichia coli isolates from Saboba district and Bolgatanga Municipality Ghana. PLoS ONE |
| title | Genomic characterization of foodborne Salmonella enterica and Escherichia coli isolates from Saboba district and Bolgatanga Municipality Ghana. |
| title_full | Genomic characterization of foodborne Salmonella enterica and Escherichia coli isolates from Saboba district and Bolgatanga Municipality Ghana. |
| title_fullStr | Genomic characterization of foodborne Salmonella enterica and Escherichia coli isolates from Saboba district and Bolgatanga Municipality Ghana. |
| title_full_unstemmed | Genomic characterization of foodborne Salmonella enterica and Escherichia coli isolates from Saboba district and Bolgatanga Municipality Ghana. |
| title_short | Genomic characterization of foodborne Salmonella enterica and Escherichia coli isolates from Saboba district and Bolgatanga Municipality Ghana. |
| title_sort | genomic characterization of foodborne salmonella enterica and escherichia coli isolates from saboba district and bolgatanga municipality ghana |
| url | https://doi.org/10.1371/journal.pone.0315583 |
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