Assessment of the hypoglycemic effect of Cyanthillium cinereum (L.) H. Rob. and its dual impact on uterine contraction in gestational diabetic rats
Objective: This study investigates the effects of Cyanthillium cinereum (L.) H. Rob. ethanolic extract (CCE) on gestational diabetes mellitus (GDM) in rats using biochemical, histological, and uterine contractility studies. Methods: Diabetes was induced in pregnant rats using streptozotocin (60 mg/k...
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| Format: | Article |
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Elsevier
2025-01-01
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| Series: | Current Research in Physiology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266594412500001X |
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| author | Sasitorn Kerdsuknirund Arreeya Kosinan Panida Khunkaewla Pakanit Kupittayanant Pattama Tongdee Porntip Nimkuntod Susan Wray Sajeera Kupittayanant |
| author_facet | Sasitorn Kerdsuknirund Arreeya Kosinan Panida Khunkaewla Pakanit Kupittayanant Pattama Tongdee Porntip Nimkuntod Susan Wray Sajeera Kupittayanant |
| author_sort | Sasitorn Kerdsuknirund |
| collection | DOAJ |
| description | Objective: This study investigates the effects of Cyanthillium cinereum (L.) H. Rob. ethanolic extract (CCE) on gestational diabetes mellitus (GDM) in rats using biochemical, histological, and uterine contractility studies. Methods: Diabetes was induced in pregnant rats using streptozotocin (60 mg/kg). CCE was administered orally at low (50 mg/kg BW) and high (500 mg/kg BW) doses from gestation day 7 to day 21. Maternal evaluations included body weight, gravid uterine weight, and biochemical assays for serum glucose, insulin, lipids, and liver enzymes. Fetal outcomes included fetal size. Histological analyses of maternal pancreatic and uterine tissues and uterine contractility studies using ex vivo muscle strip experiments were also performed. Results: CCE and metformin (MET) significantly reduced elevated blood glucose levels and improved the Islets of Langerhans area compared to the GDM group (P < 0.05). Both treatments showed a trend toward increased insulin levels (P > 0.05) and significantly reduced lipids, AST, and ALP levels (P < 0.05). High-dose CCE and MET increased gravid uterine weight and fetal size (P < 0.05) while showing a trend toward reducing placental weight and index (P > 0.05). Histological analysis revealed increased fiber area and decreased interstitial space in uterine sections (P < 0.05). Ex vivo, CCE enhanced spontaneous and oxytocin-induced contractions (P < 0.05), while MET had no effect. Conclusion: CCE reduces elevated glucose levels and exhibits hypolipidemic and hepatoprotective effects, improving maternal and fetal outcomes in GDM. Its uterine contractility effects suggest potential as a complementary therapy to MET for GDM management. |
| format | Article |
| id | doaj-art-3115af20e5ab4ecc85e01c9336a1a059 |
| institution | Kabale University |
| issn | 2665-9441 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Physiology |
| spelling | doaj-art-3115af20e5ab4ecc85e01c9336a1a0592025-02-08T05:01:09ZengElsevierCurrent Research in Physiology2665-94412025-01-018100139Assessment of the hypoglycemic effect of Cyanthillium cinereum (L.) H. Rob. and its dual impact on uterine contraction in gestational diabetic ratsSasitorn Kerdsuknirund0Arreeya Kosinan1Panida Khunkaewla2Pakanit Kupittayanant3Pattama Tongdee4Porntip Nimkuntod5Susan Wray6Sajeera Kupittayanant7School of Preclinical Sciences, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, ThailandBiochemistry-Electrochemistry Research Unit, School of Biochemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, ThailandBiochemistry-Electrochemistry Research Unit, School of Biochemistry, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, ThailandSchool of Animal Technology and Innovation, Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, 30000, ThailandSchool of Obstetrics and Gynecology, Institute of Medicine, Suranaree University of Technology, Nakhon Ratchasima, 30000, ThailandSchool of Internal Medicine, Institute of Medicine, Suranaree University of Technology, Nakhon Ratchasima, 30000, ThailandHarris-Wellbeing Preterm Birth Research Centre, Department of Women and Children's Health, The Institute of Lifecourse and Medical Sciences, University of Liverpool, Liverpool, UKSchool of Preclinical Sciences, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima, 30000, Thailand; Corresponding author. Suranaree University of Technology, 111 University Avenue, Suranaree Sub-District, Muang Nakhon Ratchasima District, Nakhon Ratchasima, 30000, Thailand.Objective: This study investigates the effects of Cyanthillium cinereum (L.) H. Rob. ethanolic extract (CCE) on gestational diabetes mellitus (GDM) in rats using biochemical, histological, and uterine contractility studies. Methods: Diabetes was induced in pregnant rats using streptozotocin (60 mg/kg). CCE was administered orally at low (50 mg/kg BW) and high (500 mg/kg BW) doses from gestation day 7 to day 21. Maternal evaluations included body weight, gravid uterine weight, and biochemical assays for serum glucose, insulin, lipids, and liver enzymes. Fetal outcomes included fetal size. Histological analyses of maternal pancreatic and uterine tissues and uterine contractility studies using ex vivo muscle strip experiments were also performed. Results: CCE and metformin (MET) significantly reduced elevated blood glucose levels and improved the Islets of Langerhans area compared to the GDM group (P < 0.05). Both treatments showed a trend toward increased insulin levels (P > 0.05) and significantly reduced lipids, AST, and ALP levels (P < 0.05). High-dose CCE and MET increased gravid uterine weight and fetal size (P < 0.05) while showing a trend toward reducing placental weight and index (P > 0.05). Histological analysis revealed increased fiber area and decreased interstitial space in uterine sections (P < 0.05). Ex vivo, CCE enhanced spontaneous and oxytocin-induced contractions (P < 0.05), while MET had no effect. Conclusion: CCE reduces elevated glucose levels and exhibits hypolipidemic and hepatoprotective effects, improving maternal and fetal outcomes in GDM. Its uterine contractility effects suggest potential as a complementary therapy to MET for GDM management.http://www.sciencedirect.com/science/article/pii/S266594412500001XGestational diabetes mellitusUterotonicTocolyticOxytocinCyanthillium cinereum (L.) H. Rob |
| spellingShingle | Sasitorn Kerdsuknirund Arreeya Kosinan Panida Khunkaewla Pakanit Kupittayanant Pattama Tongdee Porntip Nimkuntod Susan Wray Sajeera Kupittayanant Assessment of the hypoglycemic effect of Cyanthillium cinereum (L.) H. Rob. and its dual impact on uterine contraction in gestational diabetic rats Current Research in Physiology Gestational diabetes mellitus Uterotonic Tocolytic Oxytocin Cyanthillium cinereum (L.) H. Rob |
| title | Assessment of the hypoglycemic effect of Cyanthillium cinereum (L.) H. Rob. and its dual impact on uterine contraction in gestational diabetic rats |
| title_full | Assessment of the hypoglycemic effect of Cyanthillium cinereum (L.) H. Rob. and its dual impact on uterine contraction in gestational diabetic rats |
| title_fullStr | Assessment of the hypoglycemic effect of Cyanthillium cinereum (L.) H. Rob. and its dual impact on uterine contraction in gestational diabetic rats |
| title_full_unstemmed | Assessment of the hypoglycemic effect of Cyanthillium cinereum (L.) H. Rob. and its dual impact on uterine contraction in gestational diabetic rats |
| title_short | Assessment of the hypoglycemic effect of Cyanthillium cinereum (L.) H. Rob. and its dual impact on uterine contraction in gestational diabetic rats |
| title_sort | assessment of the hypoglycemic effect of cyanthillium cinereum l h rob and its dual impact on uterine contraction in gestational diabetic rats |
| topic | Gestational diabetes mellitus Uterotonic Tocolytic Oxytocin Cyanthillium cinereum (L.) H. Rob |
| url | http://www.sciencedirect.com/science/article/pii/S266594412500001X |
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