The gastric microbiome altered by A4GNT deficiency in mice
BackgroundSelective antimicrobial effects have been found for α1,4-linked N-acetylglucosamine residues at the terminus of O-glycans attached to a core protein of gastric gland mucin. A4gnt encodes α1,4-N-acetylglucosaminyl transferase, which is responsible for the biosynthesis of α1,4-linked N-acety...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2025-02-01
|
| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1541800/full |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1823856568696832000 |
|---|---|
| author | Dawei Gong Dawei Gong Yuqiang Gao Rui Shi Xiaona Xu Mengchao Yu Shumin Zhang Lili Wang Quanjiang Dong |
| author_facet | Dawei Gong Dawei Gong Yuqiang Gao Rui Shi Xiaona Xu Mengchao Yu Shumin Zhang Lili Wang Quanjiang Dong |
| author_sort | Dawei Gong |
| collection | DOAJ |
| description | BackgroundSelective antimicrobial effects have been found for α1,4-linked N-acetylglucosamine residues at the terminus of O-glycans attached to a core protein of gastric gland mucin. A4gnt encodes α1,4-N-acetylglucosaminyl transferase, which is responsible for the biosynthesis of α1,4-linked N-acetylglucosamine. The impact of A4GNT on the establishment and homeostasis of the gastric microbiome remains to be clarified. The aim of this study was to characterize the gastric microbiome in mice deficient for the production of α1,4-linked N-acetylglucosamine.MethodsThe gastric microbiome within A4gnt−/− mice and wild-type mice was analyzed using high-throughput sequencing of bacterial 16S rRNA.ResultsIn A4gnt−/− mice, which spontaneously develop gastric cancer, the community structure of the gastric microbiome was altered. The relative abundance of mutagenic Desulfovibrio and proinflammatory Prevotellamassilia in these mice was significantly increased, especially 4 weeks after birth. The co-occurrence network appeared to be much more complex. Functional prediction demonstrated considerable decreases in the relative frequencies of functions associated with polysaccharide metabolism and transportation.ConclusionThe distinct profile in A4gnt−/− mice demonstrated a vital role of A4GNT in the establishment of the gastric microbiome. A dysbiotic gastric microbiome may contribute to the spontaneous development of gastric cancer in mice. |
| format | Article |
| id | doaj-art-320acbfa7a0842d3b040078eb71db651 |
| institution | Kabale University |
| issn | 1664-302X |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj-art-320acbfa7a0842d3b040078eb71db6512025-02-12T07:25:55ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2025-02-011610.3389/fmicb.2025.15418001541800The gastric microbiome altered by A4GNT deficiency in miceDawei Gong0Dawei Gong1Yuqiang Gao2Rui Shi3Xiaona Xu4Mengchao Yu5Shumin Zhang6Lili Wang7Quanjiang Dong8Department of Gastroenterology, The Forth People's Hospital of Jinan, Jinan, ChinaCentral Laboratories, The Affiliated Qingdao Municipal Hospital of Dalian Medical University, Qingdao, ChinaCentral Laboratories, Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, ChinaQingdao Medical College, Qingdao University, Qingdao, ChinaCentral Laboratories, Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, ChinaCentral Laboratories, Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, ChinaDepartment of Gastroenterology, The Forth People's Hospital of Jinan, Jinan, ChinaCentral Laboratories, Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, ChinaCentral Laboratories, Department of Gastroenterology, Qingdao Municipal Hospital, Qingdao, ChinaBackgroundSelective antimicrobial effects have been found for α1,4-linked N-acetylglucosamine residues at the terminus of O-glycans attached to a core protein of gastric gland mucin. A4gnt encodes α1,4-N-acetylglucosaminyl transferase, which is responsible for the biosynthesis of α1,4-linked N-acetylglucosamine. The impact of A4GNT on the establishment and homeostasis of the gastric microbiome remains to be clarified. The aim of this study was to characterize the gastric microbiome in mice deficient for the production of α1,4-linked N-acetylglucosamine.MethodsThe gastric microbiome within A4gnt−/− mice and wild-type mice was analyzed using high-throughput sequencing of bacterial 16S rRNA.ResultsIn A4gnt−/− mice, which spontaneously develop gastric cancer, the community structure of the gastric microbiome was altered. The relative abundance of mutagenic Desulfovibrio and proinflammatory Prevotellamassilia in these mice was significantly increased, especially 4 weeks after birth. The co-occurrence network appeared to be much more complex. Functional prediction demonstrated considerable decreases in the relative frequencies of functions associated with polysaccharide metabolism and transportation.ConclusionThe distinct profile in A4gnt−/− mice demonstrated a vital role of A4GNT in the establishment of the gastric microbiome. A dysbiotic gastric microbiome may contribute to the spontaneous development of gastric cancer in mice.https://www.frontiersin.org/articles/10.3389/fmicb.2025.1541800/fullA4gntgastric microbiomeDesulfovibrioPrevotellamassiliagastric cancer |
| spellingShingle | Dawei Gong Dawei Gong Yuqiang Gao Rui Shi Xiaona Xu Mengchao Yu Shumin Zhang Lili Wang Quanjiang Dong The gastric microbiome altered by A4GNT deficiency in mice Frontiers in Microbiology A4gnt gastric microbiome Desulfovibrio Prevotellamassilia gastric cancer |
| title | The gastric microbiome altered by A4GNT deficiency in mice |
| title_full | The gastric microbiome altered by A4GNT deficiency in mice |
| title_fullStr | The gastric microbiome altered by A4GNT deficiency in mice |
| title_full_unstemmed | The gastric microbiome altered by A4GNT deficiency in mice |
| title_short | The gastric microbiome altered by A4GNT deficiency in mice |
| title_sort | gastric microbiome altered by a4gnt deficiency in mice |
| topic | A4gnt gastric microbiome Desulfovibrio Prevotellamassilia gastric cancer |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2025.1541800/full |
| work_keys_str_mv | AT daweigong thegastricmicrobiomealteredbya4gntdeficiencyinmice AT daweigong thegastricmicrobiomealteredbya4gntdeficiencyinmice AT yuqianggao thegastricmicrobiomealteredbya4gntdeficiencyinmice AT ruishi thegastricmicrobiomealteredbya4gntdeficiencyinmice AT xiaonaxu thegastricmicrobiomealteredbya4gntdeficiencyinmice AT mengchaoyu thegastricmicrobiomealteredbya4gntdeficiencyinmice AT shuminzhang thegastricmicrobiomealteredbya4gntdeficiencyinmice AT liliwang thegastricmicrobiomealteredbya4gntdeficiencyinmice AT quanjiangdong thegastricmicrobiomealteredbya4gntdeficiencyinmice AT daweigong gastricmicrobiomealteredbya4gntdeficiencyinmice AT daweigong gastricmicrobiomealteredbya4gntdeficiencyinmice AT yuqianggao gastricmicrobiomealteredbya4gntdeficiencyinmice AT ruishi gastricmicrobiomealteredbya4gntdeficiencyinmice AT xiaonaxu gastricmicrobiomealteredbya4gntdeficiencyinmice AT mengchaoyu gastricmicrobiomealteredbya4gntdeficiencyinmice AT shuminzhang gastricmicrobiomealteredbya4gntdeficiencyinmice AT liliwang gastricmicrobiomealteredbya4gntdeficiencyinmice AT quanjiangdong gastricmicrobiomealteredbya4gntdeficiencyinmice |