Mixture of Bacillus Amyloliquefaciens and Bacillus Pumilus Modulates Community Structures of Rice Rhizosphere Soil to Suppress Rice Seedling Blight
Rice seedling blight, caused by various fungi, including Fusarium oxysporum, poses a severe threat to rice production. As awareness grows regarding the environmental and safety hazards associated with the application of fungicides for managing rice seedling blight, there has been a shift in focus to...
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Elsevier
2025-01-01
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| Series: | Rice Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1672630824000830 |
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| author | Jiang Nan Qiu Jiehua Tian Dagang Shi Huanbin Liu Zhiquan Wen Hui Xie Shuwei Chen Huizhe Wu Meng Kou Yanjun |
| author_facet | Jiang Nan Qiu Jiehua Tian Dagang Shi Huanbin Liu Zhiquan Wen Hui Xie Shuwei Chen Huizhe Wu Meng Kou Yanjun |
| author_sort | Jiang Nan |
| collection | DOAJ |
| description | Rice seedling blight, caused by various fungi, including Fusarium oxysporum, poses a severe threat to rice production. As awareness grows regarding the environmental and safety hazards associated with the application of fungicides for managing rice seedling blight, there has been a shift in focus towards biological control agents. In this study, we isolated biocontrol bacteria from paddy fields that significantly inhibited the growth of F. oxysporum in vitro and identified the strains as Bacillus amyloliquefaciens T40 and Bacillus pumilus T208. Additionally, our findings indicated that the combined application of these Bacillus strains in soil was more effective in reducing the incidence of rice seedling blight than their individual use. Analysis of 16S and internal transcribed spacer rRNA gene sequencing data revealed that the mixture of the T40 and T208 strains exhibited the lowest average clustering coefficients, which were negatively correlated with the biomass of F. oxysporum-inoculated rice seedlings. Furthermore, this mixture led to higher stochastic assembly (average |βNTI| < 2) and reduced selection pressures on rice rhizosphere bacteria compared with individual strain applications. The mixture of the T40 and T208 strains also significantly increased the expression of defense-related genes. In conclusion, the mixture of the T40 and T208 strains effectively modulates microbial community structures, enhances microbial network stability, and boosts the resistance against rice seedling blight. Our study supports the development and utilization of biological resources for crop protection. |
| format | Article |
| id | doaj-art-33dd9fc3782141ebb54569fdaee5844c |
| institution | Kabale University |
| issn | 1672-6308 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Rice Science |
| spelling | doaj-art-33dd9fc3782141ebb54569fdaee5844c2025-02-12T05:30:48ZengElsevierRice Science1672-63082025-01-01321118130Mixture of Bacillus Amyloliquefaciens and Bacillus Pumilus Modulates Community Structures of Rice Rhizosphere Soil to Suppress Rice Seedling BlightJiang Nan0Qiu Jiehua1Tian Dagang2Shi Huanbin3Liu Zhiquan4Wen Hui5Xie Shuwei6Chen Huizhe7Wu Meng8Kou Yanjun9State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, ChinaBiotechnology Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, ChinaState Key Laboratory of Soil and Sustainable Agriculture, the Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, ChinaState Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 311400, China; Corresponding author.Rice seedling blight, caused by various fungi, including Fusarium oxysporum, poses a severe threat to rice production. As awareness grows regarding the environmental and safety hazards associated with the application of fungicides for managing rice seedling blight, there has been a shift in focus towards biological control agents. In this study, we isolated biocontrol bacteria from paddy fields that significantly inhibited the growth of F. oxysporum in vitro and identified the strains as Bacillus amyloliquefaciens T40 and Bacillus pumilus T208. Additionally, our findings indicated that the combined application of these Bacillus strains in soil was more effective in reducing the incidence of rice seedling blight than their individual use. Analysis of 16S and internal transcribed spacer rRNA gene sequencing data revealed that the mixture of the T40 and T208 strains exhibited the lowest average clustering coefficients, which were negatively correlated with the biomass of F. oxysporum-inoculated rice seedlings. Furthermore, this mixture led to higher stochastic assembly (average |βNTI| < 2) and reduced selection pressures on rice rhizosphere bacteria compared with individual strain applications. The mixture of the T40 and T208 strains also significantly increased the expression of defense-related genes. In conclusion, the mixture of the T40 and T208 strains effectively modulates microbial community structures, enhances microbial network stability, and boosts the resistance against rice seedling blight. Our study supports the development and utilization of biological resources for crop protection.http://www.sciencedirect.com/science/article/pii/S1672630824000830application strategydisease controldisease resistancemicrobial community structuremicrobial community assembly processOryza sativa |
| spellingShingle | Jiang Nan Qiu Jiehua Tian Dagang Shi Huanbin Liu Zhiquan Wen Hui Xie Shuwei Chen Huizhe Wu Meng Kou Yanjun Mixture of Bacillus Amyloliquefaciens and Bacillus Pumilus Modulates Community Structures of Rice Rhizosphere Soil to Suppress Rice Seedling Blight Rice Science application strategy disease control disease resistance microbial community structure microbial community assembly process Oryza sativa |
| title | Mixture of Bacillus Amyloliquefaciens and Bacillus Pumilus Modulates Community Structures of Rice Rhizosphere Soil to Suppress Rice Seedling Blight |
| title_full | Mixture of Bacillus Amyloliquefaciens and Bacillus Pumilus Modulates Community Structures of Rice Rhizosphere Soil to Suppress Rice Seedling Blight |
| title_fullStr | Mixture of Bacillus Amyloliquefaciens and Bacillus Pumilus Modulates Community Structures of Rice Rhizosphere Soil to Suppress Rice Seedling Blight |
| title_full_unstemmed | Mixture of Bacillus Amyloliquefaciens and Bacillus Pumilus Modulates Community Structures of Rice Rhizosphere Soil to Suppress Rice Seedling Blight |
| title_short | Mixture of Bacillus Amyloliquefaciens and Bacillus Pumilus Modulates Community Structures of Rice Rhizosphere Soil to Suppress Rice Seedling Blight |
| title_sort | mixture of bacillus amyloliquefaciens and bacillus pumilus modulates community structures of rice rhizosphere soil to suppress rice seedling blight |
| topic | application strategy disease control disease resistance microbial community structure microbial community assembly process Oryza sativa |
| url | http://www.sciencedirect.com/science/article/pii/S1672630824000830 |
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