Expression and correlation of endoglin, sEndoglin, and MMP-14 on preeclampsia placenta
Background: Hypertensive disorders, particularly preeclampsia, are major contributors to maternal mortality and neonatal morbidity. Angiogenic imbalance plays a critical role in placental ischemia, a hallmark of preeclampsia. Purpose: This study aimed to investigate the roles of endoglin, solubl...
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Indonesian Society for Biochemistry and Molecular Biology
2024-11-01
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| Series: | Acta Biochimica Indonesiana |
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| Online Access: | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/179 |
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| author | Febriana Catur Iswanti Ninik Mudjihartini Reni Paramita Yuditiya Purwosunu Ani Retno Prijanti |
| author_facet | Febriana Catur Iswanti Ninik Mudjihartini Reni Paramita Yuditiya Purwosunu Ani Retno Prijanti |
| author_sort | Febriana Catur Iswanti |
| collection | DOAJ |
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Background: Hypertensive disorders, particularly preeclampsia, are major contributors to maternal mortality and neonatal morbidity. Angiogenic imbalance plays a critical role in placental ischemia, a hallmark of preeclampsia.
Purpose: This study aimed to investigate the roles of endoglin, soluble endoglin (sEndoglin), and matrix metalloproteinase-14 (MMP-14) in the angiogenic imbalance observed in preeclampsia placentas compared to normal-term placentas.
Method: A cross-sectional study was conducted using 68 placental samples: 34 from normal-term pregnancies and 34 from preeclampsia cases. Concentrations of endoglin, sEndoglin, and MMP-14 were measured using the sandwich ELISA method, and protein levels were determined using the Christian Warburg method. Data were analyzed using SPSS version 20.
Results: The concentration of endoglin in preeclampsia placentas was slightly higher (1.37 [0.2–2.2] ng/μg protein) compared to normal placentas (1.12 [0.6–14.1] ng/μg protein), although the difference was not statistically significant. In contrast, sEndoglin (0.05 [0.0–0.01] ng/μg protein vs. 0.02 [0.0–0.3] ng/μg protein) and MMP-14 (0.14 [0.1–0.6] ng/μg protein vs. 0.11 [0.1–1.3] ng/μg protein) concentrations were significantly higher in preeclampsia placentas compared to normal placentas. All parameters showed a gradual decrease with advancing gestational age. sEndoglin and MMP-14 demonstrated a strong positive correlation (r = 0.658, p < 0.001), while endoglin and MMP-14 exhibited a moderate positive correlation (r = 0.554, p < 0.001).
Conclusion: Endoglin, sEndoglin, and MMP-14 were differentially expressed in preeclampsia placentas, with sEndoglin and MMP-14 significantly elevated. These findings highlight their potential role in angiogenic imbalance and may provide insight into the pathophysiology of preeclampsia.
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| format | Article |
| id | doaj-art-34c3f0634b274661a412f48f786c464f |
| institution | Kabale University |
| issn | 2654-6108 2654-3222 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Indonesian Society for Biochemistry and Molecular Biology |
| record_format | Article |
| series | Acta Biochimica Indonesiana |
| spelling | doaj-art-34c3f0634b274661a412f48f786c464f2025-02-08T03:04:42ZengIndonesian Society for Biochemistry and Molecular BiologyActa Biochimica Indonesiana2654-61082654-32222024-11-017210.32889/actabioina.179 Expression and correlation of endoglin, sEndoglin, and MMP-14 on preeclampsia placentaFebriana Catur Iswanti0Ninik Mudjihartini1Reni Paramita2Yuditiya Purwosunu3Ani Retno Prijanti4Department of Biochemistry and Molecular Biology, Faculty of Medicine, Universitas Indonesia, Jakarta Pusat 10340, IndonesiaDepartment of Biochemistry & Molecular Biology Faculty of Medicine Universitas Indonesia, Jakarta Pusat 10340, IndonesiaDepartment of Biochemistry & Molecular Biology Faculty of Medicine Universitas Indonesia, Jakarta Pusat 10340, IndonesiaDepartment of Obstetrics & Gynecology, Faculty of Medicine, Universitas Indonesia, Jakarta Pusat 10340, IndonesiaDepartment of Biochemistry & Molecular Biology Faculty of Medicine Universitas Indonesia, Jakarta Pusat 10340, Indonesia Background: Hypertensive disorders, particularly preeclampsia, are major contributors to maternal mortality and neonatal morbidity. Angiogenic imbalance plays a critical role in placental ischemia, a hallmark of preeclampsia. Purpose: This study aimed to investigate the roles of endoglin, soluble endoglin (sEndoglin), and matrix metalloproteinase-14 (MMP-14) in the angiogenic imbalance observed in preeclampsia placentas compared to normal-term placentas. Method: A cross-sectional study was conducted using 68 placental samples: 34 from normal-term pregnancies and 34 from preeclampsia cases. Concentrations of endoglin, sEndoglin, and MMP-14 were measured using the sandwich ELISA method, and protein levels were determined using the Christian Warburg method. Data were analyzed using SPSS version 20. Results: The concentration of endoglin in preeclampsia placentas was slightly higher (1.37 [0.2–2.2] ng/μg protein) compared to normal placentas (1.12 [0.6–14.1] ng/μg protein), although the difference was not statistically significant. In contrast, sEndoglin (0.05 [0.0–0.01] ng/μg protein vs. 0.02 [0.0–0.3] ng/μg protein) and MMP-14 (0.14 [0.1–0.6] ng/μg protein vs. 0.11 [0.1–1.3] ng/μg protein) concentrations were significantly higher in preeclampsia placentas compared to normal placentas. All parameters showed a gradual decrease with advancing gestational age. sEndoglin and MMP-14 demonstrated a strong positive correlation (r = 0.658, p < 0.001), while endoglin and MMP-14 exhibited a moderate positive correlation (r = 0.554, p < 0.001). Conclusion: Endoglin, sEndoglin, and MMP-14 were differentially expressed in preeclampsia placentas, with sEndoglin and MMP-14 significantly elevated. These findings highlight their potential role in angiogenic imbalance and may provide insight into the pathophysiology of preeclampsia. https://pbbmi.org/newjurnal/index.php/actabioina/article/view/179preeclampsiaEndoglinsEndoglinMMP-14 |
| spellingShingle | Febriana Catur Iswanti Ninik Mudjihartini Reni Paramita Yuditiya Purwosunu Ani Retno Prijanti Expression and correlation of endoglin, sEndoglin, and MMP-14 on preeclampsia placenta Acta Biochimica Indonesiana preeclampsia Endoglin sEndoglin MMP-14 |
| title | Expression and correlation of endoglin, sEndoglin, and MMP-14 on preeclampsia placenta |
| title_full | Expression and correlation of endoglin, sEndoglin, and MMP-14 on preeclampsia placenta |
| title_fullStr | Expression and correlation of endoglin, sEndoglin, and MMP-14 on preeclampsia placenta |
| title_full_unstemmed | Expression and correlation of endoglin, sEndoglin, and MMP-14 on preeclampsia placenta |
| title_short | Expression and correlation of endoglin, sEndoglin, and MMP-14 on preeclampsia placenta |
| title_sort | expression and correlation of endoglin sendoglin and mmp 14 on preeclampsia placenta |
| topic | preeclampsia Endoglin sEndoglin MMP-14 |
| url | https://pbbmi.org/newjurnal/index.php/actabioina/article/view/179 |
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