Immune imbalance in the pre-ovulatory follicular microenvironment of overweight and obese women during IVF
Abstract Background Overweight and obesity can induce an inflammatory milieu in the oocyte microenvironment and are closely associated with reduced assisted reproductive outcomes. Objective How are immune cells, cytokines and lipid profiles altered in the pre-ovulatory microenvironment of overweight...
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BMC
2025-02-01
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| Series: | Journal of Ovarian Research |
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| Online Access: | https://doi.org/10.1186/s13048-025-01606-5 |
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| author | Yang Liu Yiran Zhao Chengliang Zhou Hong Zhu Jiexue Pan Jing Fu Hefeng Huang Hui Lin Li Jin |
| author_facet | Yang Liu Yiran Zhao Chengliang Zhou Hong Zhu Jiexue Pan Jing Fu Hefeng Huang Hui Lin Li Jin |
| author_sort | Yang Liu |
| collection | DOAJ |
| description | Abstract Background Overweight and obesity can induce an inflammatory milieu in the oocyte microenvironment and are closely associated with reduced assisted reproductive outcomes. Objective How are immune cells, cytokines and lipid profiles altered in the pre-ovulatory microenvironment of overweight and obese women? Methods 32 women undergoing in vitro fertilization (IVF) were included, with 14 overweight or obese (OW) and 18 normal weight (NW) participants. Serum was collected before ovulation induction, follicular fluid (FF) and aspirates were obtained during oocyte retrieval for flow cytometry, cytokines, hormone, and lipid profiles measurement. Clinical outcomes were recorded through a one-year follow-up. Results The percentage of T cells in the pre-ovulatory follicular microenvironment, especially CD4+ T cells, increased significantly in the OW group, which positively related with BMI. Notably, type 2 cytokine IL4 and IL13 transcription level in OW group had significantly increased, while the type 1 cytokine IFNG only showed a non-statistically significant upward trend. Lipid profiles were screened, revealing no difference between the two groups, however, levels were higher in serum compared to FF. Additionally, the concentration gradient of TG between serum and FF was 22-fold in OW group (2.92 ± 3.66 vs. 0.13 ± 0.03), which was significantly higher than the 12-fold gradient observed in NW group (1.72 ± 0.95 vs. 0.14 ± 0.08). Furthermore, day 3 high quality embryos rate is negatively associated with BMI and exhibits a decreasing trend in OW group. Conclusion Overweight and obesity can disrupt immune hemostasis in the pre-ovulatory follicular microenvironment, potentially leading to adverse effects on assisted reproductive outcomes. |
| format | Article |
| id | doaj-art-35d8866d78d44df2951e917053fd11e8 |
| institution | Kabale University |
| issn | 1757-2215 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj-art-35d8866d78d44df2951e917053fd11e82025-02-09T12:49:59ZengBMCJournal of Ovarian Research1757-22152025-02-0118111010.1186/s13048-025-01606-5Immune imbalance in the pre-ovulatory follicular microenvironment of overweight and obese women during IVFYang Liu0Yiran Zhao1Chengliang Zhou2Hong Zhu3Jiexue Pan4Jing Fu5Hefeng Huang6Hui Lin7Li Jin8Obstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan UniversityObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan UniversityInternational Peace Maternity and Child Health Hospital, Shanghai Jiao Tong University School of MedicineObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan UniversityObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan UniversityShanghai JiAi Genetics and IVF Institute, Obstetrics and Gynecology Hospital, Fudan UniversityObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan UniversityObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan UniversityObstetrics and Gynecology Hospital, Institute of Reproduction and Development, Fudan UniversityAbstract Background Overweight and obesity can induce an inflammatory milieu in the oocyte microenvironment and are closely associated with reduced assisted reproductive outcomes. Objective How are immune cells, cytokines and lipid profiles altered in the pre-ovulatory microenvironment of overweight and obese women? Methods 32 women undergoing in vitro fertilization (IVF) were included, with 14 overweight or obese (OW) and 18 normal weight (NW) participants. Serum was collected before ovulation induction, follicular fluid (FF) and aspirates were obtained during oocyte retrieval for flow cytometry, cytokines, hormone, and lipid profiles measurement. Clinical outcomes were recorded through a one-year follow-up. Results The percentage of T cells in the pre-ovulatory follicular microenvironment, especially CD4+ T cells, increased significantly in the OW group, which positively related with BMI. Notably, type 2 cytokine IL4 and IL13 transcription level in OW group had significantly increased, while the type 1 cytokine IFNG only showed a non-statistically significant upward trend. Lipid profiles were screened, revealing no difference between the two groups, however, levels were higher in serum compared to FF. Additionally, the concentration gradient of TG between serum and FF was 22-fold in OW group (2.92 ± 3.66 vs. 0.13 ± 0.03), which was significantly higher than the 12-fold gradient observed in NW group (1.72 ± 0.95 vs. 0.14 ± 0.08). Furthermore, day 3 high quality embryos rate is negatively associated with BMI and exhibits a decreasing trend in OW group. Conclusion Overweight and obesity can disrupt immune hemostasis in the pre-ovulatory follicular microenvironment, potentially leading to adverse effects on assisted reproductive outcomes.https://doi.org/10.1186/s13048-025-01606-5ObesityPre-ovulatory follicular microenvironmentT cellsCholesterolIVF |
| spellingShingle | Yang Liu Yiran Zhao Chengliang Zhou Hong Zhu Jiexue Pan Jing Fu Hefeng Huang Hui Lin Li Jin Immune imbalance in the pre-ovulatory follicular microenvironment of overweight and obese women during IVF Journal of Ovarian Research Obesity Pre-ovulatory follicular microenvironment T cells Cholesterol IVF |
| title | Immune imbalance in the pre-ovulatory follicular microenvironment of overweight and obese women during IVF |
| title_full | Immune imbalance in the pre-ovulatory follicular microenvironment of overweight and obese women during IVF |
| title_fullStr | Immune imbalance in the pre-ovulatory follicular microenvironment of overweight and obese women during IVF |
| title_full_unstemmed | Immune imbalance in the pre-ovulatory follicular microenvironment of overweight and obese women during IVF |
| title_short | Immune imbalance in the pre-ovulatory follicular microenvironment of overweight and obese women during IVF |
| title_sort | immune imbalance in the pre ovulatory follicular microenvironment of overweight and obese women during ivf |
| topic | Obesity Pre-ovulatory follicular microenvironment T cells Cholesterol IVF |
| url | https://doi.org/10.1186/s13048-025-01606-5 |
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