Metagenomic next-generation sequencing assists in the diagnosis of visceral leishmaniasis in non-endemic areas of China
IntroductionLeishmaniasis, a protozoan disease caused by infection by Leishmania, is a critical issue in Asia, South America, East Africa, and North Africa. With 12 million cases globally, leishmaniasis is one of the most serious neglected tropical diseases worldwide. Direct identification of infect...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1517046/full |
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| author | Rui Zhao Guilun He Lin Xiang Melinda Ji Rongheng He Xudong Wei |
| author_facet | Rui Zhao Guilun He Lin Xiang Melinda Ji Rongheng He Xudong Wei |
| author_sort | Rui Zhao |
| collection | DOAJ |
| description | IntroductionLeishmaniasis, a protozoan disease caused by infection by Leishmania, is a critical issue in Asia, South America, East Africa, and North Africa. With 12 million cases globally, leishmaniasis is one of the most serious neglected tropical diseases worldwide. Direct identification of infected tissues is currently the primary method of diagnosis; however, the low sensitivity and inconvenience of microscopic examination in detecting amastigotes, parasitic manifestations of Leishmania, leads to the possibility of misdiagnosis, delayed diagnosis, and underdiagnosis.MethodsWith the development of metagenomic nextgeneration sequencing (mNGS) technology for pathogen identification, it is possible to detect specific nucleic acid sequences characteristic of Leishmania parasites, which opens new avenues for the more accurate diagnosis of leishmaniasis. In this study, we report two cases of leishmaniasis from Henan Province, China, in which Leishmania parasites were identified using mNGS technology, massively expediting diagnosis and treatment.ResultsOur report demonstrates that the mNGS method is applicable to peripheral blood samples (PB), which are far more readily available in clinical settings, in addition to bone marrow aspirate samples (BM), which are traditionally used for diagnosis of visceral leishmaniasis.ConclusionOur report validates the efficacy of mNGS technology as a rapid and accurate method of diagnosis for leishmaniasis. |
| format | Article |
| id | doaj-art-36111bf2393f49ab9b2451ce76024ca0 |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-36111bf2393f49ab9b2451ce76024ca02025-02-06T07:09:26ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-02-011510.3389/fcimb.2025.15170461517046Metagenomic next-generation sequencing assists in the diagnosis of visceral leishmaniasis in non-endemic areas of ChinaRui Zhao0Guilun He1Lin Xiang2Melinda Ji3Rongheng He4Xudong Wei5Department of Hematopathy, Henan Institute of Hematology, Cancer Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan, ChinaScience and Technology Service Center, Nanjing Practice Medicine Diagnostics CO., Ltd., Nanjing, Jiangsu, ChinaScience and Technology Service Center, Nanjing Practice Medicine Diagnostics CO., Ltd., Nanjing, Jiangsu, ChinaDepartment of Translational Research and Cellular Therapeutics, City of Hope, Duarte, CA, United StatesDepartment of Hematopathy, Henan Institute of Hematology, Cancer Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan, ChinaDepartment of Hematopathy, Henan Institute of Hematology, Cancer Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan, ChinaIntroductionLeishmaniasis, a protozoan disease caused by infection by Leishmania, is a critical issue in Asia, South America, East Africa, and North Africa. With 12 million cases globally, leishmaniasis is one of the most serious neglected tropical diseases worldwide. Direct identification of infected tissues is currently the primary method of diagnosis; however, the low sensitivity and inconvenience of microscopic examination in detecting amastigotes, parasitic manifestations of Leishmania, leads to the possibility of misdiagnosis, delayed diagnosis, and underdiagnosis.MethodsWith the development of metagenomic nextgeneration sequencing (mNGS) technology for pathogen identification, it is possible to detect specific nucleic acid sequences characteristic of Leishmania parasites, which opens new avenues for the more accurate diagnosis of leishmaniasis. In this study, we report two cases of leishmaniasis from Henan Province, China, in which Leishmania parasites were identified using mNGS technology, massively expediting diagnosis and treatment.ResultsOur report demonstrates that the mNGS method is applicable to peripheral blood samples (PB), which are far more readily available in clinical settings, in addition to bone marrow aspirate samples (BM), which are traditionally used for diagnosis of visceral leishmaniasis.ConclusionOur report validates the efficacy of mNGS technology as a rapid and accurate method of diagnosis for leishmaniasis.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1517046/fullmetagenomic next-generation sequencing assiststherapyleishmaniasisendemic areaclinical diagnosis |
| spellingShingle | Rui Zhao Guilun He Lin Xiang Melinda Ji Rongheng He Xudong Wei Metagenomic next-generation sequencing assists in the diagnosis of visceral leishmaniasis in non-endemic areas of China Frontiers in Cellular and Infection Microbiology metagenomic next-generation sequencing assists therapy leishmaniasis endemic area clinical diagnosis |
| title | Metagenomic next-generation sequencing assists in the diagnosis of visceral leishmaniasis in non-endemic areas of China |
| title_full | Metagenomic next-generation sequencing assists in the diagnosis of visceral leishmaniasis in non-endemic areas of China |
| title_fullStr | Metagenomic next-generation sequencing assists in the diagnosis of visceral leishmaniasis in non-endemic areas of China |
| title_full_unstemmed | Metagenomic next-generation sequencing assists in the diagnosis of visceral leishmaniasis in non-endemic areas of China |
| title_short | Metagenomic next-generation sequencing assists in the diagnosis of visceral leishmaniasis in non-endemic areas of China |
| title_sort | metagenomic next generation sequencing assists in the diagnosis of visceral leishmaniasis in non endemic areas of china |
| topic | metagenomic next-generation sequencing assists therapy leishmaniasis endemic area clinical diagnosis |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1517046/full |
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