Protocol for automated tracking and quantification of adult zebrafish anxiety behavior using ZebraTrack
Summary: The open-field tank test is a well-validated technique for evaluating anxiety behavior in vertebrate animal models. However, manual behavioral analysis can be subjective and time consuming. Here, we present a protocol using ZebraTrack, an open-source ImageJ-based approach for distraction-fr...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166725000371 |
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| author | Atanu Pramanik Shubham Nema Yogesh Bhargava Anamika Bhargava |
| author_facet | Atanu Pramanik Shubham Nema Yogesh Bhargava Anamika Bhargava |
| author_sort | Atanu Pramanik |
| collection | DOAJ |
| description | Summary: The open-field tank test is a well-validated technique for evaluating anxiety behavior in vertebrate animal models. However, manual behavioral analysis can be subjective and time consuming. Here, we present a protocol using ZebraTrack, an open-source ImageJ-based approach for distraction-free swimming video acquisition and automated tracking of adult zebrafish. We detail steps for mathematical calculations on 2D coordinates to extract various behavioral endpoints for anxiety-behavioral evaluation.For complete details on the use and execution of this protocol, please refer to Nema et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-36c4d6918fd74478b252b1bb33f8024c |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-36c4d6918fd74478b252b1bb33f8024c2025-02-12T05:32:49ZengElsevierSTAR Protocols2666-16672025-03-0161103631Protocol for automated tracking and quantification of adult zebrafish anxiety behavior using ZebraTrackAtanu Pramanik0Shubham Nema1Yogesh Bhargava2Anamika Bhargava3Department of Biotechnology, Indian Institute of Technology Hyderabad, Kandi, Sangareddy 502284, Telangana, IndiaMolecular Engineering and Imaging Lab, Department of Microbiology, Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar, MP 470003, IndiaMolecular Engineering and Imaging Lab, Department of Microbiology, Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar, MP 470003, India; Corresponding authorDepartment of Biotechnology, Indian Institute of Technology Hyderabad, Kandi, Sangareddy 502284, Telangana, India; Corresponding authorSummary: The open-field tank test is a well-validated technique for evaluating anxiety behavior in vertebrate animal models. However, manual behavioral analysis can be subjective and time consuming. Here, we present a protocol using ZebraTrack, an open-source ImageJ-based approach for distraction-free swimming video acquisition and automated tracking of adult zebrafish. We detail steps for mathematical calculations on 2D coordinates to extract various behavioral endpoints for anxiety-behavioral evaluation.For complete details on the use and execution of this protocol, please refer to Nema et al.1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166725000371Model OrganismsNeuroscienceBehavior |
| spellingShingle | Atanu Pramanik Shubham Nema Yogesh Bhargava Anamika Bhargava Protocol for automated tracking and quantification of adult zebrafish anxiety behavior using ZebraTrack STAR Protocols Model Organisms Neuroscience Behavior |
| title | Protocol for automated tracking and quantification of adult zebrafish anxiety behavior using ZebraTrack |
| title_full | Protocol for automated tracking and quantification of adult zebrafish anxiety behavior using ZebraTrack |
| title_fullStr | Protocol for automated tracking and quantification of adult zebrafish anxiety behavior using ZebraTrack |
| title_full_unstemmed | Protocol for automated tracking and quantification of adult zebrafish anxiety behavior using ZebraTrack |
| title_short | Protocol for automated tracking and quantification of adult zebrafish anxiety behavior using ZebraTrack |
| title_sort | protocol for automated tracking and quantification of adult zebrafish anxiety behavior using zebratrack |
| topic | Model Organisms Neuroscience Behavior |
| url | http://www.sciencedirect.com/science/article/pii/S2666166725000371 |
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