In situ quantification of fungicide residue on wheat leaf surfaces using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging technology
To overcome the time-consuming off-site limitations in conventional pesticide detection, this contribution presents an in situ quantitative analysis detection strategy for pesticides on leaf surfaces using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging technolog...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157525000082 |
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| author | Xuerui Yang Mengyao Shi Minghui Hong Zhixin Hui Jiaqi Pan Guangli Xiu Lei Zhou |
| author_facet | Xuerui Yang Mengyao Shi Minghui Hong Zhixin Hui Jiaqi Pan Guangli Xiu Lei Zhou |
| author_sort | Xuerui Yang |
| collection | DOAJ |
| description | To overcome the time-consuming off-site limitations in conventional pesticide detection, this contribution presents an in situ quantitative analysis detection strategy for pesticides on leaf surfaces using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging technology. Taking fungicide metrafenone as a representative, we initially screened seven commonly used matrices, and identifying α-cyano-4-hydroxycinnamic acid as the most effective one in positive mode. Subsequently, coating the matrix using sublimation spraying method resulted in the highest mass intensity. The optimal combination of operation parameters were established with imaging step size of 30 μm × 30 μm, laser diameter of 75 μm, and laser energy of 4.01 μJ/pulse. Under these conditions, a lowest detection limit of 0.6 ng/mm2 was reached for metrafenone, demonstrating extremely high sensitivity and strong repeatability. This method exhibits great potential for the analysis of various pesticides, including triadimefon and tebuconazole, thereby offering innovative approaches to the analysis of pesticide residues. |
| format | Article |
| id | doaj-art-38620ec2859b4bbca7546cbc11e2db84 |
| institution | Kabale University |
| issn | 2590-1575 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj-art-38620ec2859b4bbca7546cbc11e2db842025-02-12T05:32:18ZengElsevierFood Chemistry: X2590-15752025-01-0125102162In situ quantification of fungicide residue on wheat leaf surfaces using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging technologyXuerui Yang0Mengyao Shi1Minghui Hong2Zhixin Hui3Jiaqi Pan4Guangli Xiu5Lei Zhou6State Environmental Protection Key Lab of Environmental Risk Assessment and Control on Chemical Processes, School of Resources & Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China; Shanghai Environmental Protection Key Laboratory for Environmental Standard and Risk Management of Chemical Pollutants, School of Resources & Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, ChinaState Environmental Protection Key Lab of Environmental Risk Assessment and Control on Chemical Processes, School of Resources & Environmental Engineering, East China University of Science and Technology, Shanghai 200237, ChinaNanjing Institute of Environmental Science, Ministry of Environmental Protection of China, Nanjing 210042, ChinaState Environmental Protection Key Lab of Environmental Risk Assessment and Control on Chemical Processes, School of Resources & Environmental Engineering, East China University of Science and Technology, Shanghai 200237, ChinaState Environmental Protection Key Lab of Environmental Risk Assessment and Control on Chemical Processes, School of Resources & Environmental Engineering, East China University of Science and Technology, Shanghai 200237, ChinaState Environmental Protection Key Lab of Environmental Risk Assessment and Control on Chemical Processes, School of Resources & Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China; Shanghai Environmental Protection Key Laboratory for Environmental Standard and Risk Management of Chemical Pollutants, School of Resources & Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, ChinaState Environmental Protection Key Lab of Environmental Risk Assessment and Control on Chemical Processes, School of Resources & Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China; Shanghai Environmental Protection Key Laboratory for Environmental Standard and Risk Management of Chemical Pollutants, School of Resources & Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China; Corresponding author at: State Environmental Protection Key Lab of Environmental Risk Assessment and Control on Chemical Processes, School of Resources & Environmental Engineering, East China University of Science and Technology, Shanghai 200237, China.To overcome the time-consuming off-site limitations in conventional pesticide detection, this contribution presents an in situ quantitative analysis detection strategy for pesticides on leaf surfaces using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging technology. Taking fungicide metrafenone as a representative, we initially screened seven commonly used matrices, and identifying α-cyano-4-hydroxycinnamic acid as the most effective one in positive mode. Subsequently, coating the matrix using sublimation spraying method resulted in the highest mass intensity. The optimal combination of operation parameters were established with imaging step size of 30 μm × 30 μm, laser diameter of 75 μm, and laser energy of 4.01 μJ/pulse. Under these conditions, a lowest detection limit of 0.6 ng/mm2 was reached for metrafenone, demonstrating extremely high sensitivity and strong repeatability. This method exhibits great potential for the analysis of various pesticides, including triadimefon and tebuconazole, thereby offering innovative approaches to the analysis of pesticide residues.http://www.sciencedirect.com/science/article/pii/S2590157525000082LeafMatricesMetrafenonePesticideSublimation spraying |
| spellingShingle | Xuerui Yang Mengyao Shi Minghui Hong Zhixin Hui Jiaqi Pan Guangli Xiu Lei Zhou In situ quantification of fungicide residue on wheat leaf surfaces using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging technology Food Chemistry: X Leaf Matrices Metrafenone Pesticide Sublimation spraying |
| title | In situ quantification of fungicide residue on wheat leaf surfaces using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging technology |
| title_full | In situ quantification of fungicide residue on wheat leaf surfaces using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging technology |
| title_fullStr | In situ quantification of fungicide residue on wheat leaf surfaces using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging technology |
| title_full_unstemmed | In situ quantification of fungicide residue on wheat leaf surfaces using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging technology |
| title_short | In situ quantification of fungicide residue on wheat leaf surfaces using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry imaging technology |
| title_sort | in situ quantification of fungicide residue on wheat leaf surfaces using matrix assisted laser desorption ionization time of flight mass spectrometry imaging technology |
| topic | Leaf Matrices Metrafenone Pesticide Sublimation spraying |
| url | http://www.sciencedirect.com/science/article/pii/S2590157525000082 |
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