Dose effects of iron on growth, antioxidant potential, intestinal morphology, and intestinal barrier in yellow-feathered broilers
This experiment was conducted to investigate the dose effects of iron on growth performance, antioxidant function, small intestinal histology, and intestinal barrier of 63-day-old yellow-feathered broilers. A total of 720 1-day-old male yellow-feathered broilers were randomly divided into 9 treatmen...
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Elsevier
2025-03-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579125001026 |
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| author | J. Chen K.W. Lei S.Y. Li D.P. Li Y.L. Wang X. Wang X. Bai Y.L. Huang |
| author_facet | J. Chen K.W. Lei S.Y. Li D.P. Li Y.L. Wang X. Wang X. Bai Y.L. Huang |
| author_sort | J. Chen |
| collection | DOAJ |
| description | This experiment was conducted to investigate the dose effects of iron on growth performance, antioxidant function, small intestinal histology, and intestinal barrier of 63-day-old yellow-feathered broilers. A total of 720 1-day-old male yellow-feathered broilers were randomly divided into 9 treatments, with 8 cages per treatment and 10 birds per cage. The Fe supplementation was 0, 20, 40, 60, 80, 160, 320, 640, and 1280 mg/kg, respectively, in the form of FeSO4•7H2O. The results showed that the ADG (P = 0.002) and ADFI (P < 0.001) decreased linearly with increased dietary Fe supplementation. Malondialdehyde (MDA) concentration in plasma (P = 0.001), duodenum (P < 0.001), and jejunum (P < 0.001) were increased linearly as dietary Fe increased. As dietary Fe increased, there was a linear decrease in the villus height and the villus height/crypt depth in the duodenum (P = 0.003; P = 0.001) and jejunum (P = 0.001; P < 0.001). Decreased secretory immunoglobulin A (sIgA) concentration in jejunal mucosa (P < 0.001) was observed with increased dietary Fe concentration. Lower jejunal sIgA concentrations were observed in birds consuming more than 160 mg/kg of Fe (P < 0.001). A quadratic response was found for jejunal diamine oxidase (DAO) activity (P = 0.011) as dietary Fe supplementation was increased. The highest response of DAO in jejunal mucosa was observed for broilers supplemented with 160 mg/kg of Fe. Furthermore, the mRNA expressions of ZO-1 (P < 0.001), occludin (P = 0.004), and claudin-1 (P = 0.007) in jejunal mucosa decreased linearly with increased dietary Fe concentration. Data from the study suggests that there is no need to supplement additional Fe to a corn-soybean-based diet for yellow-feathered broilers based on growth performance, antioxidant potential, small intestinal histology, and intestinal barrier. Chronic iron exposure (≥ 160 mg/kg) can damage the intestinal barrier function, and further increase of Fe supplementation can lead to oxidative stress and even cause growth inhibition for yellow-feathered broilers. |
| format | Article |
| id | doaj-art-3c105ab37ca1467b9c7667dff409c015 |
| institution | Kabale University |
| issn | 0032-5791 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
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| series | Poultry Science |
| spelling | doaj-art-3c105ab37ca1467b9c7667dff409c0152025-02-08T04:59:18ZengElsevierPoultry Science0032-57912025-03-011043104865Dose effects of iron on growth, antioxidant potential, intestinal morphology, and intestinal barrier in yellow-feathered broilersJ. Chen0K.W. Lei1S.Y. Li2D.P. Li3Y.L. Wang4X. Wang5X. Bai6Y.L. Huang7Key Laboratory of Animal Science of State Ethnic Affairs Commission, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, PR China; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, PR ChinaKey Laboratory of Animal Science of State Ethnic Affairs Commission, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, PR China; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, PR ChinaKey Laboratory of Animal Science of State Ethnic Affairs Commission, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, PR China; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, PR ChinaKey Laboratory of Animal Science of State Ethnic Affairs Commission, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, PR China; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, PR ChinaKey Laboratory of Animal Science of State Ethnic Affairs Commission, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, PR ChinaKey Laboratory of Animal Science of State Ethnic Affairs Commission, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, PR China; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, PR ChinaKey Laboratory of Animal Science of State Ethnic Affairs Commission, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, PR China; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, PR ChinaKey Laboratory of Animal Science of State Ethnic Affairs Commission, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, PR China; Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, College of Animal and Veterinary Sciences, Southwest Minzu University, Chengdu 610041, PR China; Corresponding author.This experiment was conducted to investigate the dose effects of iron on growth performance, antioxidant function, small intestinal histology, and intestinal barrier of 63-day-old yellow-feathered broilers. A total of 720 1-day-old male yellow-feathered broilers were randomly divided into 9 treatments, with 8 cages per treatment and 10 birds per cage. The Fe supplementation was 0, 20, 40, 60, 80, 160, 320, 640, and 1280 mg/kg, respectively, in the form of FeSO4•7H2O. The results showed that the ADG (P = 0.002) and ADFI (P < 0.001) decreased linearly with increased dietary Fe supplementation. Malondialdehyde (MDA) concentration in plasma (P = 0.001), duodenum (P < 0.001), and jejunum (P < 0.001) were increased linearly as dietary Fe increased. As dietary Fe increased, there was a linear decrease in the villus height and the villus height/crypt depth in the duodenum (P = 0.003; P = 0.001) and jejunum (P = 0.001; P < 0.001). Decreased secretory immunoglobulin A (sIgA) concentration in jejunal mucosa (P < 0.001) was observed with increased dietary Fe concentration. Lower jejunal sIgA concentrations were observed in birds consuming more than 160 mg/kg of Fe (P < 0.001). A quadratic response was found for jejunal diamine oxidase (DAO) activity (P = 0.011) as dietary Fe supplementation was increased. The highest response of DAO in jejunal mucosa was observed for broilers supplemented with 160 mg/kg of Fe. Furthermore, the mRNA expressions of ZO-1 (P < 0.001), occludin (P = 0.004), and claudin-1 (P = 0.007) in jejunal mucosa decreased linearly with increased dietary Fe concentration. Data from the study suggests that there is no need to supplement additional Fe to a corn-soybean-based diet for yellow-feathered broilers based on growth performance, antioxidant potential, small intestinal histology, and intestinal barrier. Chronic iron exposure (≥ 160 mg/kg) can damage the intestinal barrier function, and further increase of Fe supplementation can lead to oxidative stress and even cause growth inhibition for yellow-feathered broilers.http://www.sciencedirect.com/science/article/pii/S0032579125001026Dose effectIronIntestinal barrierYellow-feathered broiler |
| spellingShingle | J. Chen K.W. Lei S.Y. Li D.P. Li Y.L. Wang X. Wang X. Bai Y.L. Huang Dose effects of iron on growth, antioxidant potential, intestinal morphology, and intestinal barrier in yellow-feathered broilers Poultry Science Dose effect Iron Intestinal barrier Yellow-feathered broiler |
| title | Dose effects of iron on growth, antioxidant potential, intestinal morphology, and intestinal barrier in yellow-feathered broilers |
| title_full | Dose effects of iron on growth, antioxidant potential, intestinal morphology, and intestinal barrier in yellow-feathered broilers |
| title_fullStr | Dose effects of iron on growth, antioxidant potential, intestinal morphology, and intestinal barrier in yellow-feathered broilers |
| title_full_unstemmed | Dose effects of iron on growth, antioxidant potential, intestinal morphology, and intestinal barrier in yellow-feathered broilers |
| title_short | Dose effects of iron on growth, antioxidant potential, intestinal morphology, and intestinal barrier in yellow-feathered broilers |
| title_sort | dose effects of iron on growth antioxidant potential intestinal morphology and intestinal barrier in yellow feathered broilers |
| topic | Dose effect Iron Intestinal barrier Yellow-feathered broiler |
| url | http://www.sciencedirect.com/science/article/pii/S0032579125001026 |
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