The effect of probiotic-fortified kefir on depression, appetite, oxidative stress, and inflammatory parameters in Iranian overweight and obese elderly: a randomized, double-blind, placebo-controlled clinical trial
Abstract Background It has been shown that the microflora of the gastrointestinal tract undergoes changes in obese individuals. The present study aimed to investigate the effect of kefir fortified with two strains, Lactobacillus helveticus and Bifidobacterium longum, on depression, appetite, oxidati...
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BMC
2025-02-01
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| Series: | Journal of Health, Population and Nutrition |
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| Online Access: | https://doi.org/10.1186/s41043-025-00773-x |
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| author | Mehran Noori Zainab Shateri Siavash Babajafari Mohammad Hadi Eskandari Karim Parastouei Mohammad Ghasemi Hoseein Afshari Mohammad Samadi |
| author_facet | Mehran Noori Zainab Shateri Siavash Babajafari Mohammad Hadi Eskandari Karim Parastouei Mohammad Ghasemi Hoseein Afshari Mohammad Samadi |
| author_sort | Mehran Noori |
| collection | DOAJ |
| description | Abstract Background It has been shown that the microflora of the gastrointestinal tract undergoes changes in obese individuals. The present study aimed to investigate the effect of kefir fortified with two strains, Lactobacillus helveticus and Bifidobacterium longum, on depression, appetite, oxidative stress, and inflammatory parameters in overweight and obese elderly individuals. Methods This study was a double-blind, randomized, and placebo-controlled clinical trial conducted on 67 elderly men aged over 65, who were randomly divided into two groups. One group (n = 35) received one bottle (240 cc) of regular kefir as a placebo, while the intervention group (n = 32) received one bottle of probiotic-fortified kefir for eight weeks. Depression and appetite were evaluated using the Geriatric Depression Scale-15 (GDS-15) and a validated Visual Analogue Scale (VAS), respectively. Oxidative stress parameters were assessed using the standard calorimetric method, and inflammatory parameters were measured via the enzyme-linked immunosorbent assay method (ELISA). The differences between the two groups were compared using the independent samples T-test. Results The median age of participant in both groups was 65 years. A significant difference in depression scores and the mean change between the two groups was observed after eight weeks (p = 0.001 and p = 0.042, respectively). Within-group comparison revealed a significant increase in appetite scores in both groups (p < 0.05 for both). Moreover, a significant difference in the changes in total antioxidant capacity (TAC) was noted (p = 0.009). However, no significant differences were observed in other oxidative and inflammatory parameters between the two groups (p˃0.05 for all). Conclusions The results demonstrated the positive impact of two specific strains of Bifidobacterium and Lactobacillus on improving depression in the elderly. However, when comparing the two groups, no significant effects were observed on appetite, inflammation, and oxidative stress parameters, except for TAC. |
| format | Article |
| id | doaj-art-3c57cde89a8a47b2a0228ec606bf589c |
| institution | Kabale University |
| issn | 2072-1315 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | BMC |
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| series | Journal of Health, Population and Nutrition |
| spelling | doaj-art-3c57cde89a8a47b2a0228ec606bf589c2025-02-09T12:43:00ZengBMCJournal of Health, Population and Nutrition2072-13152025-02-0144111010.1186/s41043-025-00773-xThe effect of probiotic-fortified kefir on depression, appetite, oxidative stress, and inflammatory parameters in Iranian overweight and obese elderly: a randomized, double-blind, placebo-controlled clinical trialMehran Noori0Zainab Shateri1Siavash Babajafari2Mohammad Hadi Eskandari3Karim Parastouei4Mohammad Ghasemi5Hoseein Afshari6Mohammad Samadi7Student Research Committee, Baqiyatallah University of Medical SciencesDepartment of Nutrition and Biochemistry, School of Medicine, Ilam University of Medical SciencesNutrition Research Center, Department of Clinical Nutrition, School of Nutrition and Food Science, Shiraz University of Medical SciencesDepartment of Food Science and Technology, School of Agriculture, Shiraz UniversityHealth Research Center, Life Style Institute, Baqiyatallah University of Medical SciencesHealth Research Center, Life Style Institute, Baqiyatallah University of Medical SciencesStudent Research Committee, Baqiyatallah University of Medical SciencesExercise Physiology Research Center, Life Style Institute, Baqiyatallah University of Medical SciencesAbstract Background It has been shown that the microflora of the gastrointestinal tract undergoes changes in obese individuals. The present study aimed to investigate the effect of kefir fortified with two strains, Lactobacillus helveticus and Bifidobacterium longum, on depression, appetite, oxidative stress, and inflammatory parameters in overweight and obese elderly individuals. Methods This study was a double-blind, randomized, and placebo-controlled clinical trial conducted on 67 elderly men aged over 65, who were randomly divided into two groups. One group (n = 35) received one bottle (240 cc) of regular kefir as a placebo, while the intervention group (n = 32) received one bottle of probiotic-fortified kefir for eight weeks. Depression and appetite were evaluated using the Geriatric Depression Scale-15 (GDS-15) and a validated Visual Analogue Scale (VAS), respectively. Oxidative stress parameters were assessed using the standard calorimetric method, and inflammatory parameters were measured via the enzyme-linked immunosorbent assay method (ELISA). The differences between the two groups were compared using the independent samples T-test. Results The median age of participant in both groups was 65 years. A significant difference in depression scores and the mean change between the two groups was observed after eight weeks (p = 0.001 and p = 0.042, respectively). Within-group comparison revealed a significant increase in appetite scores in both groups (p < 0.05 for both). Moreover, a significant difference in the changes in total antioxidant capacity (TAC) was noted (p = 0.009). However, no significant differences were observed in other oxidative and inflammatory parameters between the two groups (p˃0.05 for all). Conclusions The results demonstrated the positive impact of two specific strains of Bifidobacterium and Lactobacillus on improving depression in the elderly. However, when comparing the two groups, no significant effects were observed on appetite, inflammation, and oxidative stress parameters, except for TAC.https://doi.org/10.1186/s41043-025-00773-xProbioticsKefirLactobacillusBifidobacteriumDepressionOxidative stress |
| spellingShingle | Mehran Noori Zainab Shateri Siavash Babajafari Mohammad Hadi Eskandari Karim Parastouei Mohammad Ghasemi Hoseein Afshari Mohammad Samadi The effect of probiotic-fortified kefir on depression, appetite, oxidative stress, and inflammatory parameters in Iranian overweight and obese elderly: a randomized, double-blind, placebo-controlled clinical trial Journal of Health, Population and Nutrition Probiotics Kefir Lactobacillus Bifidobacterium Depression Oxidative stress |
| title | The effect of probiotic-fortified kefir on depression, appetite, oxidative stress, and inflammatory parameters in Iranian overweight and obese elderly: a randomized, double-blind, placebo-controlled clinical trial |
| title_full | The effect of probiotic-fortified kefir on depression, appetite, oxidative stress, and inflammatory parameters in Iranian overweight and obese elderly: a randomized, double-blind, placebo-controlled clinical trial |
| title_fullStr | The effect of probiotic-fortified kefir on depression, appetite, oxidative stress, and inflammatory parameters in Iranian overweight and obese elderly: a randomized, double-blind, placebo-controlled clinical trial |
| title_full_unstemmed | The effect of probiotic-fortified kefir on depression, appetite, oxidative stress, and inflammatory parameters in Iranian overweight and obese elderly: a randomized, double-blind, placebo-controlled clinical trial |
| title_short | The effect of probiotic-fortified kefir on depression, appetite, oxidative stress, and inflammatory parameters in Iranian overweight and obese elderly: a randomized, double-blind, placebo-controlled clinical trial |
| title_sort | effect of probiotic fortified kefir on depression appetite oxidative stress and inflammatory parameters in iranian overweight and obese elderly a randomized double blind placebo controlled clinical trial |
| topic | Probiotics Kefir Lactobacillus Bifidobacterium Depression Oxidative stress |
| url | https://doi.org/10.1186/s41043-025-00773-x |
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