Characterization of Escherichia coli outer membrane vesicles and the impact of pathogenic ones on NLR signaling pathways
Background and Objectives: The secretion of outer membrane vesicles (OMVs) is a universal event among bacteria. In this study, we characterized OMVs from pathogenic and non-pathogenic strains of Escherichia coli and assessed the effect of pathogenic OMVs on NLR signaling pathways. Materials and Met...
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Tehran University of Medical Sciences
2025-02-01
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| Series: | Iranian Journal of Microbiology |
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| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/4893 |
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| author | Roghayeh Mohammadzadeh Ava Behrouzi Farzam Vaziri Seyed Davar Siadat |
| author_facet | Roghayeh Mohammadzadeh Ava Behrouzi Farzam Vaziri Seyed Davar Siadat |
| author_sort | Roghayeh Mohammadzadeh |
| collection | DOAJ |
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Background and Objectives: The secretion of outer membrane vesicles (OMVs) is a universal event among bacteria. In this study, we characterized OMVs from pathogenic and non-pathogenic strains of Escherichia coli and assessed the effect of pathogenic OMVs on NLR signaling pathways.
Materials and Methods: OMVs were extracted by differential centrifugation and characterized by scanning electron microscopy (SEM), SDS-PAGE, Limulus amebocyte lysate (LAL) test, and nucleic acid extraction. Then, the Caco-2 cells were treated with the pathogenic OMVs to evaluate their effect on NLR signaling pathways.
Results: SEM showed that pathogenic and non-pathogenic strains produced OMVs in the range of 9-72.9 and 45-270 nm, respectively. The SDS-PAGE revealed that both OMVs had protein bands ranging from 25 to 100 kDa. The LAL test displayed that the concentration of LPS was 2.368 and 0.055 EU/ml in pathogenic and non-pathogenic OMVs, respectively. The evaluation of nucleic acid contents showed no significant difference between both types of OMVs. The assessment of pathogenic OMVs' effect on NLR genes demonstrated that the expression level was changed in some genes.
Conclusion: The characterization of OMVs showed that both strains of E. coli secrete OMVs in different sizes and contents. Besides, it was revealed that OMVs can regulate gene expression.
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| format | Article |
| id | doaj-art-3e4a0e49320a4f1e8bffb6132b697863 |
| institution | Kabale University |
| issn | 2008-3289 2008-4447 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj-art-3e4a0e49320a4f1e8bffb6132b6978632025-02-09T09:01:31ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472025-02-01171Characterization of Escherichia coli outer membrane vesicles and the impact of pathogenic ones on NLR signaling pathwaysRoghayeh Mohammadzadeh0Ava Behrouzi1Farzam Vaziri2Seyed Davar Siadat3Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; Microbiology Research Center, Pasteur Institute of Iran, Tehran, IranDepartment of Microbiology, Faculty of Advanced Science and Technology, Tehran Medical Science, Islamic Azad University, Tehran, IranDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; Microbiology Research Center, Pasteur Institute of Iran, Tehran, IranDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran Background and Objectives: The secretion of outer membrane vesicles (OMVs) is a universal event among bacteria. In this study, we characterized OMVs from pathogenic and non-pathogenic strains of Escherichia coli and assessed the effect of pathogenic OMVs on NLR signaling pathways. Materials and Methods: OMVs were extracted by differential centrifugation and characterized by scanning electron microscopy (SEM), SDS-PAGE, Limulus amebocyte lysate (LAL) test, and nucleic acid extraction. Then, the Caco-2 cells were treated with the pathogenic OMVs to evaluate their effect on NLR signaling pathways. Results: SEM showed that pathogenic and non-pathogenic strains produced OMVs in the range of 9-72.9 and 45-270 nm, respectively. The SDS-PAGE revealed that both OMVs had protein bands ranging from 25 to 100 kDa. The LAL test displayed that the concentration of LPS was 2.368 and 0.055 EU/ml in pathogenic and non-pathogenic OMVs, respectively. The evaluation of nucleic acid contents showed no significant difference between both types of OMVs. The assessment of pathogenic OMVs' effect on NLR genes demonstrated that the expression level was changed in some genes. Conclusion: The characterization of OMVs showed that both strains of E. coli secrete OMVs in different sizes and contents. Besides, it was revealed that OMVs can regulate gene expression. https://ijm.tums.ac.ir/index.php/ijm/article/view/4893Escherichia coli;Outer membrane vesicles;NOD-like receptors;Signaling pathways |
| spellingShingle | Roghayeh Mohammadzadeh Ava Behrouzi Farzam Vaziri Seyed Davar Siadat Characterization of Escherichia coli outer membrane vesicles and the impact of pathogenic ones on NLR signaling pathways Iranian Journal of Microbiology Escherichia coli; Outer membrane vesicles; NOD-like receptors; Signaling pathways |
| title | Characterization of Escherichia coli outer membrane vesicles and the impact of pathogenic ones on NLR signaling pathways |
| title_full | Characterization of Escherichia coli outer membrane vesicles and the impact of pathogenic ones on NLR signaling pathways |
| title_fullStr | Characterization of Escherichia coli outer membrane vesicles and the impact of pathogenic ones on NLR signaling pathways |
| title_full_unstemmed | Characterization of Escherichia coli outer membrane vesicles and the impact of pathogenic ones on NLR signaling pathways |
| title_short | Characterization of Escherichia coli outer membrane vesicles and the impact of pathogenic ones on NLR signaling pathways |
| title_sort | characterization of escherichia coli outer membrane vesicles and the impact of pathogenic ones on nlr signaling pathways |
| topic | Escherichia coli; Outer membrane vesicles; NOD-like receptors; Signaling pathways |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/4893 |
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