Updates on hosts and distribution of pepper yellow leaf curl Indonesia virus and squash leaf curl China virus in Central Java Province, Indonesia
Chili pepper, tomato, and squash cultivated in Magelang Regency, Central Java Province, Indonesia showed severe viral symptoms. Three samples were taken then molecularly tested against begomovirus, potyvirus, tobamovirus, and polerovirus. However, PCR test confirmed only begomovirus infections. BL...
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Universitas Lancang Kuning
2024-03-01
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| Series: | Jurnal Ilmiah Pertanian |
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| Online Access: | https://dev-journal.unilak.ac.id/index.php/jip/article/view/18856 |
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| author | Adyatma Irawan Santosa Adenisa Hanifah Irbati Geby Nandita Cahyaning Pratiwi Antama Surwadinata Putri Laeshita Krisnanda Surya Dharma Reko Saputra Jaya Annisa Lisa Andriyani Chilya Qurrota A'yun Chusnu Naura Syifa Fatika |
| author_facet | Adyatma Irawan Santosa Adenisa Hanifah Irbati Geby Nandita Cahyaning Pratiwi Antama Surwadinata Putri Laeshita Krisnanda Surya Dharma Reko Saputra Jaya Annisa Lisa Andriyani Chilya Qurrota A'yun Chusnu Naura Syifa Fatika |
| author_sort | Adyatma Irawan Santosa |
| collection | DOAJ |
| description |
Chili pepper, tomato, and squash cultivated in Magelang Regency, Central Java Province, Indonesia showed severe viral symptoms. Three samples were taken then molecularly tested against begomovirus, potyvirus, tobamovirus, and polerovirus. However, PCR test confirmed only begomovirus infections. BLAST analysis concluded that the chili pepper and tomato isolates were pepper yellow leaf curl Indonesia virus (PepYLCIV) while the squash isolate was squash leaf curl China virus (SLCCNV). The 552 bp partial AV1 gene sequences of the three isolates were given NCBI GenBank acc nos. OR924278-80. PepYLCIV OR924279 and OR924280 formed a subgroup with LC542629 from Bali in the phylogenetic tree constructed using MEGA11, and shared 95.8 – 96.7% identities at nucleotide (nt) and 98.4 – 99.5% at amino acid (aa) levels according to Sequence Demarcation Tool v1.2 software. Meanwhile, SLCCNV OR924278 was clustered, and shared 95.8 – 96.7% nt and 98.4 – 99.5% aa identities with three Malaysian isolates (MW248685, MW248687, and MW248689). Different plant species inoculated with SLCCNV OR924278 remained symptomless up to four weeks observation, suggesting that the isolate is not mechanically transmissible. This study contributed additional knowledge on molecular variation while expanding hosts and distribution of PepYLCIV and SLCCNV in Central Java Province.
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| format | Article |
| id | doaj-art-3f0ed8f516ba4653b06df7dd0da4d3c9 |
| institution | Kabale University |
| issn | 1829-8346 2502-5988 |
| language | English |
| publishDate | 2024-03-01 |
| publisher | Universitas Lancang Kuning |
| record_format | Article |
| series | Jurnal Ilmiah Pertanian |
| spelling | doaj-art-3f0ed8f516ba4653b06df7dd0da4d3c92025-02-07T09:32:23ZengUniversitas Lancang KuningJurnal Ilmiah Pertanian1829-83462502-59882024-03-01211Updates on hosts and distribution of pepper yellow leaf curl Indonesia virus and squash leaf curl China virus in Central Java Province, IndonesiaAdyatma Irawan Santosa0Adenisa Hanifah Irbati1Geby Nandita Cahyaning Pratiwi2Antama Surwadinata3Putri Laeshita4Krisnanda Surya Dharma5Reko Saputra Jaya6Annisa Lisa Andriyani7Chilya Qurrota A'yun8Chusnu Naura Syifa Fatika9Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Agrotechnology, Faculty of Agriculture, Universitas Tidar, Magelang 56114, IndonesiaDirectorate of Horticulture Protection, Directorate General of Horticulture, Ministry of Agriculture, DKI Jakarta, IndonesiaDepartment of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta 55281, IndonesiaDepartment of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia Chili pepper, tomato, and squash cultivated in Magelang Regency, Central Java Province, Indonesia showed severe viral symptoms. Three samples were taken then molecularly tested against begomovirus, potyvirus, tobamovirus, and polerovirus. However, PCR test confirmed only begomovirus infections. BLAST analysis concluded that the chili pepper and tomato isolates were pepper yellow leaf curl Indonesia virus (PepYLCIV) while the squash isolate was squash leaf curl China virus (SLCCNV). The 552 bp partial AV1 gene sequences of the three isolates were given NCBI GenBank acc nos. OR924278-80. PepYLCIV OR924279 and OR924280 formed a subgroup with LC542629 from Bali in the phylogenetic tree constructed using MEGA11, and shared 95.8 – 96.7% identities at nucleotide (nt) and 98.4 – 99.5% at amino acid (aa) levels according to Sequence Demarcation Tool v1.2 software. Meanwhile, SLCCNV OR924278 was clustered, and shared 95.8 – 96.7% nt and 98.4 – 99.5% aa identities with three Malaysian isolates (MW248685, MW248687, and MW248689). Different plant species inoculated with SLCCNV OR924278 remained symptomless up to four weeks observation, suggesting that the isolate is not mechanically transmissible. This study contributed additional knowledge on molecular variation while expanding hosts and distribution of PepYLCIV and SLCCNV in Central Java Province. https://dev-journal.unilak.ac.id/index.php/jip/article/view/18856coat proteinmechanical inoculationmolecular characterizationpercentage identityphylogenetic analysis |
| spellingShingle | Adyatma Irawan Santosa Adenisa Hanifah Irbati Geby Nandita Cahyaning Pratiwi Antama Surwadinata Putri Laeshita Krisnanda Surya Dharma Reko Saputra Jaya Annisa Lisa Andriyani Chilya Qurrota A'yun Chusnu Naura Syifa Fatika Updates on hosts and distribution of pepper yellow leaf curl Indonesia virus and squash leaf curl China virus in Central Java Province, Indonesia Jurnal Ilmiah Pertanian coat protein mechanical inoculation molecular characterization percentage identity phylogenetic analysis |
| title | Updates on hosts and distribution of pepper yellow leaf curl Indonesia virus and squash leaf curl China virus in Central Java Province, Indonesia |
| title_full | Updates on hosts and distribution of pepper yellow leaf curl Indonesia virus and squash leaf curl China virus in Central Java Province, Indonesia |
| title_fullStr | Updates on hosts and distribution of pepper yellow leaf curl Indonesia virus and squash leaf curl China virus in Central Java Province, Indonesia |
| title_full_unstemmed | Updates on hosts and distribution of pepper yellow leaf curl Indonesia virus and squash leaf curl China virus in Central Java Province, Indonesia |
| title_short | Updates on hosts and distribution of pepper yellow leaf curl Indonesia virus and squash leaf curl China virus in Central Java Province, Indonesia |
| title_sort | updates on hosts and distribution of pepper yellow leaf curl indonesia virus and squash leaf curl china virus in central java province indonesia |
| topic | coat protein mechanical inoculation molecular characterization percentage identity phylogenetic analysis |
| url | https://dev-journal.unilak.ac.id/index.php/jip/article/view/18856 |
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