Identifying the function of novel cross-species microRNAs from the excretory-secretory products of Angiostrongylus cantonensis fifth-stage larvae
Background: Angiostrongylus cantonensis is a significant foodborne zoonotic parasite that causes severe neuropathological damage and symptoms in humans. Excretory-secretory products (ESPs) play a pivotal role in elucidating host-parasite interactions and can aid in penetrating host defensive barrier...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Journal of Microbiology, Immunology and Infection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S168411822400207X |
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| author | Kuang-Yao Chen Yi-Hsuan Lin Chien-Ju Cheng Yi-Hao Huang Sheng-Yu Lin Chyi-Liang Chen Cheng-Hsun Chiu |
| author_facet | Kuang-Yao Chen Yi-Hsuan Lin Chien-Ju Cheng Yi-Hao Huang Sheng-Yu Lin Chyi-Liang Chen Cheng-Hsun Chiu |
| author_sort | Kuang-Yao Chen |
| collection | DOAJ |
| description | Background: Angiostrongylus cantonensis is a significant foodborne zoonotic parasite that causes severe neuropathological damage and symptoms in humans. Excretory-secretory products (ESPs) play a pivotal role in elucidating host-parasite interactions and can aid in penetrating host defensive barriers in helminths. Recently, secreted microRNAs have become important research targets for parasite-host communication. In this study, we determined the expression and function of novel microRNAs from A. cantonensis L5 ESPs and evaluated the effect of target microRNAs on the molecular mechanisms of mouse astrocytes. Methods: Here, we employed next-generation sequencing (NGS) to establish the secreted microRNAs dataset. Next, we evaluated the effects of AcESPs-microRNAs in A. cantonensis ESPs treated astrocytes. Results: First, we established the secreted microRNA dataset, and then comprehensively verified the characteristics. Novel microRNAs were initially detected, and their expression was found. Moreover, the prediction results showed that these secreted microRNAs may regulate Wnt and mTOR signaling. Next, the data showed that the AcNOVEL55 microRNA reduced cell apoptosis generation via regulating the RhoA-Rock signaling pathway in A. cantonensis L5 ESPs treated mouse astrocytes. Moreover, we also demonstrated that the AcNOVEL31 microRNA can affect the inflammation activation via regulating the presenilin-1/GSK3B/β-catenin/NF-κB pathway. Finally, the concentrations of secreted IL-6 and IL-12 proteins were downregulated by AcNOVEL31 microRNA by influencing presenilin-1 expression. Conclusion: This is the first study to verify the molecular functions of novel microRNAs secreted by A. cantonensis. The discovery of the microRNA mechanisms by which cross-species parasitic nematodes influence hosts has advanced research on host-parasitic nematode interactions. |
| format | Article |
| id | doaj-art-411850fde3a6417cbc5d61c3ec923e02 |
| institution | Kabale University |
| issn | 1684-1182 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Microbiology, Immunology and Infection |
| spelling | doaj-art-411850fde3a6417cbc5d61c3ec923e022025-02-06T05:11:20ZengElsevierJournal of Microbiology, Immunology and Infection1684-11822025-02-01581128137Identifying the function of novel cross-species microRNAs from the excretory-secretory products of Angiostrongylus cantonensis fifth-stage larvaeKuang-Yao Chen0Yi-Hsuan Lin1Chien-Ju Cheng2Yi-Hao Huang3Sheng-Yu Lin4Chyi-Liang Chen5Cheng-Hsun Chiu6Department of Parasitology, College of Medicine, Chang Gung University, Taoyuan, 333, Taiwan; Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, 333, Taiwan; Molecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Taoyuan, Taiwan; Corresponding author. Department of Parasitology, College of Medicine, Chang Gung University, Taoyuan, 333, Taiwan.Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, 333, TaiwanDepartment of Parasitology, College of Medicine, Chang Gung University, Taoyuan, 333, TaiwanGraduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, 333, TaiwanGraduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taoyuan, 333, TaiwanMolecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Taoyuan, TaiwanMolecular Infectious Disease Research Center, Chang Gung Memorial Hospital, Taoyuan, TaiwanBackground: Angiostrongylus cantonensis is a significant foodborne zoonotic parasite that causes severe neuropathological damage and symptoms in humans. Excretory-secretory products (ESPs) play a pivotal role in elucidating host-parasite interactions and can aid in penetrating host defensive barriers in helminths. Recently, secreted microRNAs have become important research targets for parasite-host communication. In this study, we determined the expression and function of novel microRNAs from A. cantonensis L5 ESPs and evaluated the effect of target microRNAs on the molecular mechanisms of mouse astrocytes. Methods: Here, we employed next-generation sequencing (NGS) to establish the secreted microRNAs dataset. Next, we evaluated the effects of AcESPs-microRNAs in A. cantonensis ESPs treated astrocytes. Results: First, we established the secreted microRNA dataset, and then comprehensively verified the characteristics. Novel microRNAs were initially detected, and their expression was found. Moreover, the prediction results showed that these secreted microRNAs may regulate Wnt and mTOR signaling. Next, the data showed that the AcNOVEL55 microRNA reduced cell apoptosis generation via regulating the RhoA-Rock signaling pathway in A. cantonensis L5 ESPs treated mouse astrocytes. Moreover, we also demonstrated that the AcNOVEL31 microRNA can affect the inflammation activation via regulating the presenilin-1/GSK3B/β-catenin/NF-κB pathway. Finally, the concentrations of secreted IL-6 and IL-12 proteins were downregulated by AcNOVEL31 microRNA by influencing presenilin-1 expression. Conclusion: This is the first study to verify the molecular functions of novel microRNAs secreted by A. cantonensis. The discovery of the microRNA mechanisms by which cross-species parasitic nematodes influence hosts has advanced research on host-parasitic nematode interactions.http://www.sciencedirect.com/science/article/pii/S168411822400207XAngiostrongylus cantonensisExcretory-secretory productsNovel microRNAsHelminth-host interactionRhoAPresenilin-1 |
| spellingShingle | Kuang-Yao Chen Yi-Hsuan Lin Chien-Ju Cheng Yi-Hao Huang Sheng-Yu Lin Chyi-Liang Chen Cheng-Hsun Chiu Identifying the function of novel cross-species microRNAs from the excretory-secretory products of Angiostrongylus cantonensis fifth-stage larvae Journal of Microbiology, Immunology and Infection Angiostrongylus cantonensis Excretory-secretory products Novel microRNAs Helminth-host interaction RhoA Presenilin-1 |
| title | Identifying the function of novel cross-species microRNAs from the excretory-secretory products of Angiostrongylus cantonensis fifth-stage larvae |
| title_full | Identifying the function of novel cross-species microRNAs from the excretory-secretory products of Angiostrongylus cantonensis fifth-stage larvae |
| title_fullStr | Identifying the function of novel cross-species microRNAs from the excretory-secretory products of Angiostrongylus cantonensis fifth-stage larvae |
| title_full_unstemmed | Identifying the function of novel cross-species microRNAs from the excretory-secretory products of Angiostrongylus cantonensis fifth-stage larvae |
| title_short | Identifying the function of novel cross-species microRNAs from the excretory-secretory products of Angiostrongylus cantonensis fifth-stage larvae |
| title_sort | identifying the function of novel cross species micrornas from the excretory secretory products of angiostrongylus cantonensis fifth stage larvae |
| topic | Angiostrongylus cantonensis Excretory-secretory products Novel microRNAs Helminth-host interaction RhoA Presenilin-1 |
| url | http://www.sciencedirect.com/science/article/pii/S168411822400207X |
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