Specialized contact sites regulate the fusion of chlamydial inclusion membranes
Abstract The intracellular bacterial pathogen Chlamydia trachomatis replicates within a membrane-bound compartment called the inclusion. Upon infection with several chlamydiae, each bacterium creates its own inclusion, resulting in multiple inclusions within each host cell. Ultimately, these inclusi...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-10-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-53443-7 |
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| _version_ | 1823861794670641152 |
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| author | Christine Linton Jordan Wesolowski Anna Lobley Toshiyuki Yamaji Kentaro Hanada Fabienne Paumet |
| author_facet | Christine Linton Jordan Wesolowski Anna Lobley Toshiyuki Yamaji Kentaro Hanada Fabienne Paumet |
| author_sort | Christine Linton |
| collection | DOAJ |
| description | Abstract The intracellular bacterial pathogen Chlamydia trachomatis replicates within a membrane-bound compartment called the inclusion. Upon infection with several chlamydiae, each bacterium creates its own inclusion, resulting in multiple inclusions within each host cell. Ultimately, these inclusions fuse together in a process that requires the chlamydial protein IncA. Here, we show that inclusions form unique contact sites (inclusion contact sites, ICSs) prior to fusion, that serve as fusogenic platforms in which specific lipids and chlamydial proteins concentrate. Fusion depends on IncA clustering within ICSs and is regulated by PI(3,4)P2 and sphingolipids. As IncA concentrates within ICSs, its C-terminus likely interacts in trans with IncA on the apposing membrane, securing a high concentration of IncA at fusion sites. This regulatory mechanism contrasts with eukaryotic or viral fusion systems that are either composed of multiple proteins or use a change in pH to initiate membrane fusion. Thus, our study demonstrates that Chlamydia-mediated membrane fusion is primarily regulated by specific structural domains in IncA and its local organization on the inclusion membrane, which is affected by the host cell lipid composition. |
| format | Article |
| id | doaj-art-4160d72d259849d09a16ef92d3ec033b |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-4160d72d259849d09a16ef92d3ec033b2025-02-09T12:43:48ZengNature PortfolioNature Communications2041-17232024-10-0115111510.1038/s41467-024-53443-7Specialized contact sites regulate the fusion of chlamydial inclusion membranesChristine Linton0Jordan Wesolowski1Anna Lobley2Toshiyuki Yamaji3Kentaro Hanada4Fabienne Paumet5Department of Microbiology and Immunology, Thomas Jefferson UniversityDepartment of Microbiology and Immunology, Thomas Jefferson UniversityDepartment of Microbiology and Immunology, Thomas Jefferson UniversityDepartment of Microbiology and Immunology, Faculty of Pharmacy, Juntendo UniversityCenter for Quality Management Systems, National Institute of Infectious DiseasesDepartment of Microbiology and Immunology, Thomas Jefferson UniversityAbstract The intracellular bacterial pathogen Chlamydia trachomatis replicates within a membrane-bound compartment called the inclusion. Upon infection with several chlamydiae, each bacterium creates its own inclusion, resulting in multiple inclusions within each host cell. Ultimately, these inclusions fuse together in a process that requires the chlamydial protein IncA. Here, we show that inclusions form unique contact sites (inclusion contact sites, ICSs) prior to fusion, that serve as fusogenic platforms in which specific lipids and chlamydial proteins concentrate. Fusion depends on IncA clustering within ICSs and is regulated by PI(3,4)P2 and sphingolipids. As IncA concentrates within ICSs, its C-terminus likely interacts in trans with IncA on the apposing membrane, securing a high concentration of IncA at fusion sites. This regulatory mechanism contrasts with eukaryotic or viral fusion systems that are either composed of multiple proteins or use a change in pH to initiate membrane fusion. Thus, our study demonstrates that Chlamydia-mediated membrane fusion is primarily regulated by specific structural domains in IncA and its local organization on the inclusion membrane, which is affected by the host cell lipid composition.https://doi.org/10.1038/s41467-024-53443-7 |
| spellingShingle | Christine Linton Jordan Wesolowski Anna Lobley Toshiyuki Yamaji Kentaro Hanada Fabienne Paumet Specialized contact sites regulate the fusion of chlamydial inclusion membranes Nature Communications |
| title | Specialized contact sites regulate the fusion of chlamydial inclusion membranes |
| title_full | Specialized contact sites regulate the fusion of chlamydial inclusion membranes |
| title_fullStr | Specialized contact sites regulate the fusion of chlamydial inclusion membranes |
| title_full_unstemmed | Specialized contact sites regulate the fusion of chlamydial inclusion membranes |
| title_short | Specialized contact sites regulate the fusion of chlamydial inclusion membranes |
| title_sort | specialized contact sites regulate the fusion of chlamydial inclusion membranes |
| url | https://doi.org/10.1038/s41467-024-53443-7 |
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