Molecular self-assembly strategy tuning a dry crosslinking protein patch for biocompatible and biodegradable haemostatic sealing
Abstract Uncontrolled haemorrhage is a leading cause of trauma-related fatalities, highlighting the critical need for rapid and effective haemostasis. Current haemostatic materials encounter limitations such as slow clotting and weak mechanical strength, while most of bioadhesives compromise their a...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-56726-9 |
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| _version_ | 1823861795684614144 |
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| author | Lisha Yu Zhaodi Liu Yong Zheng Zongrui Tong Yihang Ding Weilin Wang Yuan Ding Zhengwei Mao |
| author_facet | Lisha Yu Zhaodi Liu Yong Zheng Zongrui Tong Yihang Ding Weilin Wang Yuan Ding Zhengwei Mao |
| author_sort | Lisha Yu |
| collection | DOAJ |
| description | Abstract Uncontrolled haemorrhage is a leading cause of trauma-related fatalities, highlighting the critical need for rapid and effective haemostasis. Current haemostatic materials encounter limitations such as slow clotting and weak mechanical strength, while most of bioadhesives compromise their adhesion performance to wet tissues for biocompatibility and degradability. In this study, a molecular self-assembly strategy is proposed, developing a biocompatible and biodegradable protein-based patch with excellent adhesion performance. This strategy utilizes fibrinogen modified with hydrophobic groups to induce self-assembly into a hydrogel, which is converted into a dry patch. The protein patch enhances adhesion performance on the wet tissue through a dry cross-linking method and robust intra/inter-molecular interactions. This patch demonstrates excellent haemostatic efficacy in both porcine oozing wound and porcine severe acute haemorrhage. It maintains biological functionality, and ensures sustained wound sealing while gradually degrading in vivo, making it a promising candidate for clinical tissue sealing applications. |
| format | Article |
| id | doaj-art-433cd1d6a5bc4a81a9fdad410c277a5a |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-433cd1d6a5bc4a81a9fdad410c277a5a2025-02-09T12:45:25ZengNature PortfolioNature Communications2041-17232025-02-0116111310.1038/s41467-025-56726-9Molecular self-assembly strategy tuning a dry crosslinking protein patch for biocompatible and biodegradable haemostatic sealingLisha Yu0Zhaodi Liu1Yong Zheng2Zongrui Tong3Yihang Ding4Weilin Wang5Yuan Ding6Zhengwei Mao7Department of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang UniversityMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang UniversityMOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang UniversityDepartment of Hepatobiliary and Pancreatic Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang UniversityAbstract Uncontrolled haemorrhage is a leading cause of trauma-related fatalities, highlighting the critical need for rapid and effective haemostasis. Current haemostatic materials encounter limitations such as slow clotting and weak mechanical strength, while most of bioadhesives compromise their adhesion performance to wet tissues for biocompatibility and degradability. In this study, a molecular self-assembly strategy is proposed, developing a biocompatible and biodegradable protein-based patch with excellent adhesion performance. This strategy utilizes fibrinogen modified with hydrophobic groups to induce self-assembly into a hydrogel, which is converted into a dry patch. The protein patch enhances adhesion performance on the wet tissue through a dry cross-linking method and robust intra/inter-molecular interactions. This patch demonstrates excellent haemostatic efficacy in both porcine oozing wound and porcine severe acute haemorrhage. It maintains biological functionality, and ensures sustained wound sealing while gradually degrading in vivo, making it a promising candidate for clinical tissue sealing applications.https://doi.org/10.1038/s41467-025-56726-9 |
| spellingShingle | Lisha Yu Zhaodi Liu Yong Zheng Zongrui Tong Yihang Ding Weilin Wang Yuan Ding Zhengwei Mao Molecular self-assembly strategy tuning a dry crosslinking protein patch for biocompatible and biodegradable haemostatic sealing Nature Communications |
| title | Molecular self-assembly strategy tuning a dry crosslinking protein patch for biocompatible and biodegradable haemostatic sealing |
| title_full | Molecular self-assembly strategy tuning a dry crosslinking protein patch for biocompatible and biodegradable haemostatic sealing |
| title_fullStr | Molecular self-assembly strategy tuning a dry crosslinking protein patch for biocompatible and biodegradable haemostatic sealing |
| title_full_unstemmed | Molecular self-assembly strategy tuning a dry crosslinking protein patch for biocompatible and biodegradable haemostatic sealing |
| title_short | Molecular self-assembly strategy tuning a dry crosslinking protein patch for biocompatible and biodegradable haemostatic sealing |
| title_sort | molecular self assembly strategy tuning a dry crosslinking protein patch for biocompatible and biodegradable haemostatic sealing |
| url | https://doi.org/10.1038/s41467-025-56726-9 |
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