Diagnostic Effectiveness of Transforming Growth Factor Beta 1 in Assessing the Risk of Developing Graft Dysfunction in Liver Recipient Children
The problem of non-invasive monitoring of the liver condition is particularly relevant in liver transplantation among young children. Transforming growth factor beta 1 (TGF-β1) is a pleiotropic cytokine with a profibrogenic and immunosuppressive effect that can have a definite effect on the liver tr...
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Gastro LLC
2019-06-01
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| Series: | Российский журнал гастроэнтерологии, гепатологии, колопроктологии |
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| Online Access: | https://www.gastro-j.ru/jour/article/view/340 |
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| author | R. M. Kurabekova O. M. Tsiroulnikova I. E. Pashkova A. G. Olefirenko O. E. Gichkun L. V. Makarova R. A. Monakhov P. O. Shevchenko |
| author_facet | R. M. Kurabekova O. M. Tsiroulnikova I. E. Pashkova A. G. Olefirenko O. E. Gichkun L. V. Makarova R. A. Monakhov P. O. Shevchenko |
| author_sort | R. M. Kurabekova |
| collection | DOAJ |
| description | The problem of non-invasive monitoring of the liver condition is particularly relevant in liver transplantation among young children. Transforming growth factor beta 1 (TGF-β1) is a pleiotropic cytokine with a profibrogenic and immunosuppressive effect that can have a definite effect on the liver transplant functioning.Aim. To determine the diagnostic efficacy of TGF-β1 in blood when assessing the risk of developing graft dysfunction in liver recipient children. Materials and methods. 95 children aged 3 to 73 months with liver cirrhosis of various etiologies were surveyed. All the patients underwent liver transplantation (LT) from a living related donor. The TGF-β1 concentration in plasma was determined using ELISA on average 3 ± 2 days prior to liver transplantation and in the early post-transplant period.Results. The level of TGF-β1 in the blood of children with liver cirrhosis was lower than in healthy children (p = 0.001). LT was accompanied by an increase in the TGF-β1 content in the blood of recipients (p = 0.001). The incidence of graft dysfunction in the early postoperative period correlated with the pre-transplantation level of TGF-β1 (r = 0.40, p = 0.00), which was lower in recipients with developed graft dysfunction than in patients without dysfunction (1.7 ± 1.3 ng/ml versus 6.7 ± 5.3 ng/ml, p = 0.001). The analysis of the test diagnostic efficiency showed that the area under the ROC curve (AUC) was 0.85 ± 0.05, 95 % CI 0.75–0.94, the sensitivity of the method was 83 %, its specificity was 77 %. When the marker value was less than the threshold (2.2 ng/ml), the relative risk of developing graft dysfunction was 11.4 ± 0.7, 95 % CI 2.7-48.7. The accuracy of the method, the positive predictive value and the negative predictive value of the results were 78, 83 and 77 %, respectively. Conclusion. The level of TGF-β1 in the blood of liver recipient children before transplantation below 2.2 ng/ml increases the risk of developing graft dysfunction in the early postoperative period 11-fold. Measuring the TGF-β1 level in the blood prior to liver transplantation makes it possible to identify recipients with 85 % chance of developing a graft dysfunction. |
| format | Article |
| id | doaj-art-433d5c7417944cda883a2d31a833c66d |
| institution | Kabale University |
| issn | 1382-4376 2658-6673 |
| language | Russian |
| publishDate | 2019-06-01 |
| publisher | Gastro LLC |
| record_format | Article |
| series | Российский журнал гастроэнтерологии, гепатологии, колопроктологии |
| spelling | doaj-art-433d5c7417944cda883a2d31a833c66d2025-02-10T16:14:35ZrusGastro LLCРоссийский журнал гастроэнтерологии, гепатологии, колопроктологии1382-43762658-66732019-06-01293586510.22416/1382-4376-2019-29-3-58-65295Diagnostic Effectiveness of Transforming Growth Factor Beta 1 in Assessing the Risk of Developing Graft Dysfunction in Liver Recipient ChildrenR. M. Kurabekova0O. M. Tsiroulnikova1I. E. Pashkova2A. G. Olefirenko3O. E. Gichkun4L. V. Makarova5R. A. Monakhov6P. O. Shevchenko7V.I. Shumakov National Medical Research Center of Transplantology and Artificial OrgansV.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs; I.M. Sechenov First Moscow State Medical University (Sechenov University)V.I. Shumakov National Medical Research Center of Transplantology and Artificial OrgansV.I. Shumakov National Medical Research Center of Transplantology and Artificial OrgansV.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs; I.M. Sechenov First Moscow State Medical University (Sechenov University)V.I. Shumakov National Medical Research Center of Transplantology and Artificial OrgansV.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs; I.M. Sechenov First Moscow State Medical University (Sechenov University)V.I. Shumakov National Medical Research Center of Transplantology and Artificial Organs; I.M. Sechenov First Moscow State Medical University (Sechenov University)The problem of non-invasive monitoring of the liver condition is particularly relevant in liver transplantation among young children. Transforming growth factor beta 1 (TGF-β1) is a pleiotropic cytokine with a profibrogenic and immunosuppressive effect that can have a definite effect on the liver transplant functioning.Aim. To determine the diagnostic efficacy of TGF-β1 in blood when assessing the risk of developing graft dysfunction in liver recipient children. Materials and methods. 95 children aged 3 to 73 months with liver cirrhosis of various etiologies were surveyed. All the patients underwent liver transplantation (LT) from a living related donor. The TGF-β1 concentration in plasma was determined using ELISA on average 3 ± 2 days prior to liver transplantation and in the early post-transplant period.Results. The level of TGF-β1 in the blood of children with liver cirrhosis was lower than in healthy children (p = 0.001). LT was accompanied by an increase in the TGF-β1 content in the blood of recipients (p = 0.001). The incidence of graft dysfunction in the early postoperative period correlated with the pre-transplantation level of TGF-β1 (r = 0.40, p = 0.00), which was lower in recipients with developed graft dysfunction than in patients without dysfunction (1.7 ± 1.3 ng/ml versus 6.7 ± 5.3 ng/ml, p = 0.001). The analysis of the test diagnostic efficiency showed that the area under the ROC curve (AUC) was 0.85 ± 0.05, 95 % CI 0.75–0.94, the sensitivity of the method was 83 %, its specificity was 77 %. When the marker value was less than the threshold (2.2 ng/ml), the relative risk of developing graft dysfunction was 11.4 ± 0.7, 95 % CI 2.7-48.7. The accuracy of the method, the positive predictive value and the negative predictive value of the results were 78, 83 and 77 %, respectively. Conclusion. The level of TGF-β1 in the blood of liver recipient children before transplantation below 2.2 ng/ml increases the risk of developing graft dysfunction in the early postoperative period 11-fold. Measuring the TGF-β1 level in the blood prior to liver transplantation makes it possible to identify recipients with 85 % chance of developing a graft dysfunction.https://www.gastro-j.ru/jour/article/view/340biomarkerliver fibrosisliver transplantationtransforming growth factor beta 1graft dysfunction |
| spellingShingle | R. M. Kurabekova O. M. Tsiroulnikova I. E. Pashkova A. G. Olefirenko O. E. Gichkun L. V. Makarova R. A. Monakhov P. O. Shevchenko Diagnostic Effectiveness of Transforming Growth Factor Beta 1 in Assessing the Risk of Developing Graft Dysfunction in Liver Recipient Children Российский журнал гастроэнтерологии, гепатологии, колопроктологии biomarker liver fibrosis liver transplantation transforming growth factor beta 1 graft dysfunction |
| title | Diagnostic Effectiveness of Transforming Growth Factor Beta 1 in Assessing the Risk of Developing Graft Dysfunction in Liver Recipient Children |
| title_full | Diagnostic Effectiveness of Transforming Growth Factor Beta 1 in Assessing the Risk of Developing Graft Dysfunction in Liver Recipient Children |
| title_fullStr | Diagnostic Effectiveness of Transforming Growth Factor Beta 1 in Assessing the Risk of Developing Graft Dysfunction in Liver Recipient Children |
| title_full_unstemmed | Diagnostic Effectiveness of Transforming Growth Factor Beta 1 in Assessing the Risk of Developing Graft Dysfunction in Liver Recipient Children |
| title_short | Diagnostic Effectiveness of Transforming Growth Factor Beta 1 in Assessing the Risk of Developing Graft Dysfunction in Liver Recipient Children |
| title_sort | diagnostic effectiveness of transforming growth factor beta 1 in assessing the risk of developing graft dysfunction in liver recipient children |
| topic | biomarker liver fibrosis liver transplantation transforming growth factor beta 1 graft dysfunction |
| url | https://www.gastro-j.ru/jour/article/view/340 |
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