Identification of key chromium resistance genes in Cellulomonas using transcriptomics
Cellulomonas fimi Clb-11 can reduce high toxic Cr(VI) to less toxic Cr(III), and transcriptomics was used to reveal the key Cr(VI) uptake and reduction genes of C. fimi Clb-11 in this study. The results showed that under 0.5 mM Cr(VI) stress, 654 genes were upregulated. Among the upregulated genes,...
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Elsevier
2025-02-01
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| Series: | Ecotoxicology and Environmental Safety |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651325001794 |
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| author | Hongmei Sun Yongdong Li Shengchao Gao Guangchuan Shi Lianbin Cao Xinya Li Tong Li Tongbiao Li Mingcheng Wang Enzhong Li Junhe Liu Hongxia Ni Yi Chen Ying Liu |
| author_facet | Hongmei Sun Yongdong Li Shengchao Gao Guangchuan Shi Lianbin Cao Xinya Li Tong Li Tongbiao Li Mingcheng Wang Enzhong Li Junhe Liu Hongxia Ni Yi Chen Ying Liu |
| author_sort | Hongmei Sun |
| collection | DOAJ |
| description | Cellulomonas fimi Clb-11 can reduce high toxic Cr(VI) to less toxic Cr(III), and transcriptomics was used to reveal the key Cr(VI) uptake and reduction genes of C. fimi Clb-11 in this study. The results showed that under 0.5 mM Cr(VI) stress, 654 genes were upregulated. Among the upregulated genes, phosphate transport protein encoding genes phoU and TC.PIT, and molybdate transport protein encoding genes modA, modB, and modC were involved in the cell uptake of Cr(VI). Cytochrome c subunits encoding genes qcrA and qcrC were involved in the intracellular reduction of Cr(VI), and cytochrome c oxidase subunits encoding genes coxB and coxC were involved in extracellular electron secretion. Additionally, several unreported genes were found to be upregulated in C. fimi Clb-11 under Cr(VI) stress. The upregulated manganese transport protein encoding gene mntH may also assist Cr(VI) uptake in C. fimi Clb-11. Proton pump subunit encoding genes atpA, atpB, atpE, atpF, and atpH, as well as sodium-hydrogen antiporter subunit encoding genes mnhA and mnhC were upregulated, it may be involved in the extracellular proton secretion to reduce Cr(VI). Iron complex transport system substrate-binding protein encoding gene ABC.FEV.S, vacuolar iron transporter encoding gene VIT, FMN reductase gene encoding gene ssuE, and quinone oxidoreductase encoding genes qor and qorB were upregulated to reduce Cr(VI) in the intracellular. Our study showed theoretical importance by revealing the Cr(VI) reduction mechanism using chrome-resistant microorganisms. |
| format | Article |
| id | doaj-art-44d8e1c9b249401b96d90cb882497bf3 |
| institution | Kabale University |
| issn | 0147-6513 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj-art-44d8e1c9b249401b96d90cb882497bf32025-02-07T04:46:36ZengElsevierEcotoxicology and Environmental Safety0147-65132025-02-01291117843Identification of key chromium resistance genes in Cellulomonas using transcriptomicsHongmei Sun0Yongdong Li1Shengchao Gao2Guangchuan Shi3Lianbin Cao4Xinya Li5Tong Li6Tongbiao Li7Mingcheng Wang8Enzhong Li9Junhe Liu10Hongxia Ni11Yi Chen12Ying Liu13College of Life Sciences, Northwest A&F University, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, No. 22 Xinong Road, Yangling, Shaanxi 712100, PR China; College of Biological and Food Engineering, Huanghuai University, No.76 Kaiyuan Road, Zhumadian, Henan 463000, PR ChinaNingbo Municipal Center for Disease Control and Prevention, Ningbo Key Laboratory of Virus Research, Ningbo 315010, PR ChinaCollege of Life Sciences, Northwest A&F University, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, No. 22 Xinong Road, Yangling, Shaanxi 712100, PR ChinaCollege of Biological and Food Engineering, Huanghuai University, No.76 Kaiyuan Road, Zhumadian, Henan 463000, PR ChinaCollege of Biological and Food Engineering, Huanghuai University, No.76 Kaiyuan Road, Zhumadian, Henan 463000, PR ChinaCollege of Biological and Food Engineering, Huanghuai University, No.76 Kaiyuan Road, Zhumadian, Henan 463000, PR ChinaCollege of Biological and Food Engineering, Huanghuai University, No.76 Kaiyuan Road, Zhumadian, Henan 463000, PR ChinaCollege of Biological and Food Engineering, Huanghuai University, No.76 Kaiyuan Road, Zhumadian, Henan 463000, PR ChinaCollege of Biological and Food Engineering, Huanghuai University, No.76 Kaiyuan Road, Zhumadian, Henan 463000, PR ChinaCollege of Biological and Food Engineering, Huanghuai University, No.76 Kaiyuan Road, Zhumadian, Henan 463000, PR ChinaCollege of Biological and Food Engineering, Huanghuai University, No.76 Kaiyuan Road, Zhumadian, Henan 463000, PR ChinaNingbo Municipal Center for Disease Control and Prevention, Ningbo Key Laboratory of Virus Research, Ningbo 315010, PR ChinaNingbo Municipal Center for Disease Control and Prevention, Ningbo Key Laboratory of Virus Research, Ningbo 315010, PR China; Corresponding authors.College of Life Sciences, Northwest A&F University, Shaanxi Key Laboratory of Agricultural and Environmental Microbiology, No. 22 Xinong Road, Yangling, Shaanxi 712100, PR China; Corresponding authors.Cellulomonas fimi Clb-11 can reduce high toxic Cr(VI) to less toxic Cr(III), and transcriptomics was used to reveal the key Cr(VI) uptake and reduction genes of C. fimi Clb-11 in this study. The results showed that under 0.5 mM Cr(VI) stress, 654 genes were upregulated. Among the upregulated genes, phosphate transport protein encoding genes phoU and TC.PIT, and molybdate transport protein encoding genes modA, modB, and modC were involved in the cell uptake of Cr(VI). Cytochrome c subunits encoding genes qcrA and qcrC were involved in the intracellular reduction of Cr(VI), and cytochrome c oxidase subunits encoding genes coxB and coxC were involved in extracellular electron secretion. Additionally, several unreported genes were found to be upregulated in C. fimi Clb-11 under Cr(VI) stress. The upregulated manganese transport protein encoding gene mntH may also assist Cr(VI) uptake in C. fimi Clb-11. Proton pump subunit encoding genes atpA, atpB, atpE, atpF, and atpH, as well as sodium-hydrogen antiporter subunit encoding genes mnhA and mnhC were upregulated, it may be involved in the extracellular proton secretion to reduce Cr(VI). Iron complex transport system substrate-binding protein encoding gene ABC.FEV.S, vacuolar iron transporter encoding gene VIT, FMN reductase gene encoding gene ssuE, and quinone oxidoreductase encoding genes qor and qorB were upregulated to reduce Cr(VI) in the intracellular. Our study showed theoretical importance by revealing the Cr(VI) reduction mechanism using chrome-resistant microorganisms.http://www.sciencedirect.com/science/article/pii/S0147651325001794Cellulomonas fimiHeavy metalsKey genes identificationHexavalent chromium reductionBioremediation |
| spellingShingle | Hongmei Sun Yongdong Li Shengchao Gao Guangchuan Shi Lianbin Cao Xinya Li Tong Li Tongbiao Li Mingcheng Wang Enzhong Li Junhe Liu Hongxia Ni Yi Chen Ying Liu Identification of key chromium resistance genes in Cellulomonas using transcriptomics Ecotoxicology and Environmental Safety Cellulomonas fimi Heavy metals Key genes identification Hexavalent chromium reduction Bioremediation |
| title | Identification of key chromium resistance genes in Cellulomonas using transcriptomics |
| title_full | Identification of key chromium resistance genes in Cellulomonas using transcriptomics |
| title_fullStr | Identification of key chromium resistance genes in Cellulomonas using transcriptomics |
| title_full_unstemmed | Identification of key chromium resistance genes in Cellulomonas using transcriptomics |
| title_short | Identification of key chromium resistance genes in Cellulomonas using transcriptomics |
| title_sort | identification of key chromium resistance genes in cellulomonas using transcriptomics |
| topic | Cellulomonas fimi Heavy metals Key genes identification Hexavalent chromium reduction Bioremediation |
| url | http://www.sciencedirect.com/science/article/pii/S0147651325001794 |
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