The epididymal microenvironment in Akodon cursor (Muridae, Sigmodontinae, Akodontini): The lifetime of spermatozoa from production to maturation
Sperm fertility in mammals depends on the complexity of several physiological events. The male gamete is produced in the testis and matured in the epididymis, which requires an adequate environment for sperm maturation and survival. This study focused on characterizing the sperm morphology of the sm...
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Elsevier
2025-01-01
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| Series: | Theriogenology Wild |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2773093X25000029 |
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| author | Tatiana Prata Menezes Fernanda Carolina Ribeiro Dias Nara Clara Lazaroni e Merchid Camila Folly Batista Camilo Jose Ramirez-Lopez Arabela Guedes de Azevedo Viana Renner Philipe Rodrigues Carvalho Adrielli Ribeiro Araújo José Lino-Neto Gisele Lessa Arlindo A. Moura Mariana Machado-Neves |
| author_facet | Tatiana Prata Menezes Fernanda Carolina Ribeiro Dias Nara Clara Lazaroni e Merchid Camila Folly Batista Camilo Jose Ramirez-Lopez Arabela Guedes de Azevedo Viana Renner Philipe Rodrigues Carvalho Adrielli Ribeiro Araújo José Lino-Neto Gisele Lessa Arlindo A. Moura Mariana Machado-Neves |
| author_sort | Tatiana Prata Menezes |
| collection | DOAJ |
| description | Sperm fertility in mammals depends on the complexity of several physiological events. The male gamete is produced in the testis and matured in the epididymis, which requires an adequate environment for sperm maturation and survival. This study focused on characterizing the sperm morphology of the small wild rodent Akodon cursor and describing the epididymal environment supporting sperm maturation. Eight A. cursor males were captured from a preserved area of the Atlantic Forest in Viçosa (MG, Brazil). The epididymides were processed for histological, functional, and proteomic analyses, besides sperm quality evaluation. Data were submitted to ANOVA and the post hoc Tukey's test (P < 0.05). The length of A. cursor sperm was 52.71 ± 0.79 μm (mean ± SD), with 5.12 ± 0.07 μm corresponding to the nucleus. Sperm motility and vigor were 45.6 % ± 2.9 and 2.9 ± 0.2, respectively. The duration of sperm transit time in the epididymis was 2.75 ± 0.22 days. Histomorphometrically, the distal cauda had the highest tubular and luminal diameter, whereas the epithelial height was higher in the initial segment than in the other regions. Proteomic analysis identified 56 proteins in the proximal epididymal regions of A. cursor related to crucial processes, including cellular homeostasis, flagellar elongation, and the elimination of defective sperm during epididymal transit. These findings provide valuable insights into the molecular mechanisms underlying sperm maturation in this species. In conclusion, the epididymis of A. cursor shares similarities with other rodent species but exhibits unique characteristics, such as shorter sperm transit time. |
| format | Article |
| id | doaj-art-44d9a5d9434f461fbeb9a3a5031b5bab |
| institution | Kabale University |
| issn | 2773-093X |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Theriogenology Wild |
| spelling | doaj-art-44d9a5d9434f461fbeb9a3a5031b5bab2025-02-08T05:01:46ZengElsevierTheriogenology Wild2773-093X2025-01-016100120The epididymal microenvironment in Akodon cursor (Muridae, Sigmodontinae, Akodontini): The lifetime of spermatozoa from production to maturationTatiana Prata Menezes0Fernanda Carolina Ribeiro Dias1Nara Clara Lazaroni e Merchid2Camila Folly Batista3Camilo Jose Ramirez-Lopez4Arabela Guedes de Azevedo Viana5Renner Philipe Rodrigues Carvalho6Adrielli Ribeiro Araújo7José Lino-Neto8Gisele Lessa9Arlindo A. Moura10Mariana Machado-Neves11Department of General Biology, Federal University of Viçosa, Viçosa, MG 36570-900, Brazil; School of Nutrition, Federal University of Ouro Preto, Ouro Preto, MG 35400-000, BrazilDepartment of General Biology, Federal University of Viçosa, Viçosa, MG 36570-900, Brazil; Federal University of Triângulo Mineiro, Uberaba, MG 38025-180, BrazilDepartment of General Biology, Federal University of Viçosa, Viçosa, MG 36570-900, BrazilDepartment of General Biology, Federal University of Viçosa, Viçosa, MG 36570-900, BrazilDepartment of General Biology, Federal University of Viçosa, Viçosa, MG 36570-900, BrazilDepartment of General Biology, Federal University of Viçosa, Viçosa, MG 36570-900, BrazilDepartment of General Biology, Federal University of Viçosa, Viçosa, MG 36570-900, BrazilDepartment of Animal Biology, Federal University of Viçosa, Viçosa, MG 36570-000, BrazilDepartment of General Biology, Federal University of Viçosa, Viçosa, MG 36570-900, BrazilDepartment of Animal Biology, Federal University of Viçosa, Viçosa, MG 36570-000, BrazilDepartment of Animal Science, Federal University of Ceará, Ceará, CE 60021-970, BrazilDepartment of General Biology, Federal University of Viçosa, Viçosa, MG 36570-900, Brazil; Correspondence to: Department of General Biology, Federal University of Viçosa, Campus Universitario, s/n, Viçosa, MG 36570-900, Brazil.Sperm fertility in mammals depends on the complexity of several physiological events. The male gamete is produced in the testis and matured in the epididymis, which requires an adequate environment for sperm maturation and survival. This study focused on characterizing the sperm morphology of the small wild rodent Akodon cursor and describing the epididymal environment supporting sperm maturation. Eight A. cursor males were captured from a preserved area of the Atlantic Forest in Viçosa (MG, Brazil). The epididymides were processed for histological, functional, and proteomic analyses, besides sperm quality evaluation. Data were submitted to ANOVA and the post hoc Tukey's test (P < 0.05). The length of A. cursor sperm was 52.71 ± 0.79 μm (mean ± SD), with 5.12 ± 0.07 μm corresponding to the nucleus. Sperm motility and vigor were 45.6 % ± 2.9 and 2.9 ± 0.2, respectively. The duration of sperm transit time in the epididymis was 2.75 ± 0.22 days. Histomorphometrically, the distal cauda had the highest tubular and luminal diameter, whereas the epithelial height was higher in the initial segment than in the other regions. Proteomic analysis identified 56 proteins in the proximal epididymal regions of A. cursor related to crucial processes, including cellular homeostasis, flagellar elongation, and the elimination of defective sperm during epididymal transit. These findings provide valuable insights into the molecular mechanisms underlying sperm maturation in this species. In conclusion, the epididymis of A. cursor shares similarities with other rodent species but exhibits unique characteristics, such as shorter sperm transit time.http://www.sciencedirect.com/science/article/pii/S2773093X25000029EpididymisMorphologyProteomeReproductionSpermatozoaSperm production |
| spellingShingle | Tatiana Prata Menezes Fernanda Carolina Ribeiro Dias Nara Clara Lazaroni e Merchid Camila Folly Batista Camilo Jose Ramirez-Lopez Arabela Guedes de Azevedo Viana Renner Philipe Rodrigues Carvalho Adrielli Ribeiro Araújo José Lino-Neto Gisele Lessa Arlindo A. Moura Mariana Machado-Neves The epididymal microenvironment in Akodon cursor (Muridae, Sigmodontinae, Akodontini): The lifetime of spermatozoa from production to maturation Theriogenology Wild Epididymis Morphology Proteome Reproduction Spermatozoa Sperm production |
| title | The epididymal microenvironment in Akodon cursor (Muridae, Sigmodontinae, Akodontini): The lifetime of spermatozoa from production to maturation |
| title_full | The epididymal microenvironment in Akodon cursor (Muridae, Sigmodontinae, Akodontini): The lifetime of spermatozoa from production to maturation |
| title_fullStr | The epididymal microenvironment in Akodon cursor (Muridae, Sigmodontinae, Akodontini): The lifetime of spermatozoa from production to maturation |
| title_full_unstemmed | The epididymal microenvironment in Akodon cursor (Muridae, Sigmodontinae, Akodontini): The lifetime of spermatozoa from production to maturation |
| title_short | The epididymal microenvironment in Akodon cursor (Muridae, Sigmodontinae, Akodontini): The lifetime of spermatozoa from production to maturation |
| title_sort | epididymal microenvironment in akodon cursor muridae sigmodontinae akodontini the lifetime of spermatozoa from production to maturation |
| topic | Epididymis Morphology Proteome Reproduction Spermatozoa Sperm production |
| url | http://www.sciencedirect.com/science/article/pii/S2773093X25000029 |
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