Bioprinting of bespoke islet-specific niches to promote maturation of stem cell-derived islets
Abstract Pancreatic islets are densely packed cellular aggregates containing various hormonal cell types essential for blood glucose regulation. Interactions among these cells markedly affect the glucoregulatory functions of islets along with the surrounding niche and pancreatic tissue-specific geom...
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| Format: | Article |
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Nature Portfolio
2025-02-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-56665-5 |
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| author | Myungji Kim Seungyeon Cho Dong Gyu Hwang In Kyong Shim Song Cheol Kim Jiwon Jang Jinah Jang |
| author_facet | Myungji Kim Seungyeon Cho Dong Gyu Hwang In Kyong Shim Song Cheol Kim Jiwon Jang Jinah Jang |
| author_sort | Myungji Kim |
| collection | DOAJ |
| description | Abstract Pancreatic islets are densely packed cellular aggregates containing various hormonal cell types essential for blood glucose regulation. Interactions among these cells markedly affect the glucoregulatory functions of islets along with the surrounding niche and pancreatic tissue-specific geometrical organization. However, stem cell (SC)-derived islets generated in vitro often lack the three-dimensional extracellular microenvironment and peri-vasculature, which leads to the immaturity of SC-derived islets, reducing their ability to detect glucose fluctuations and insulin release. Here, we bioengineer the in vivo-like pancreatic niches by optimizing the combination of pancreatic tissue-specific extracellular matrix and basement membrane proteins and utilizing bioprinting-based geometrical guidance to recreate the spatial pattern of islet peripheries. The bioprinted islet-specific niche promotes coordinated interactions between islets and vasculature, supporting structural and functional features resembling native islets. Our strategy not only improves SC-derived islet functionality but also offers significant potential for advancing research on islet development, maturation, and diabetic disease modeling, with future implications for translational applications. |
| format | Article |
| id | doaj-art-453bba7c46e541219293ee6627f6cbb3 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-453bba7c46e541219293ee6627f6cbb32025-02-09T12:45:20ZengNature PortfolioNature Communications2041-17232025-02-0116111810.1038/s41467-025-56665-5Bioprinting of bespoke islet-specific niches to promote maturation of stem cell-derived isletsMyungji Kim0Seungyeon Cho1Dong Gyu Hwang2In Kyong Shim3Song Cheol Kim4Jiwon Jang5Jinah Jang6Division of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH)Center for 3D Organ Printing and Stem Cells, Pohang University of Science and Technology (POSTECH)Center for 3D Organ Printing and Stem Cells, Pohang University of Science and Technology (POSTECH)Asan Institute for Life Science, University of Ulsan College of Medicine and Asan Medical CenterAsan Institute for Life Science, University of Ulsan College of Medicine and Asan Medical CenterCenter for 3D Organ Printing and Stem Cells, Pohang University of Science and Technology (POSTECH)Division of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology (POSTECH)Abstract Pancreatic islets are densely packed cellular aggregates containing various hormonal cell types essential for blood glucose regulation. Interactions among these cells markedly affect the glucoregulatory functions of islets along with the surrounding niche and pancreatic tissue-specific geometrical organization. However, stem cell (SC)-derived islets generated in vitro often lack the three-dimensional extracellular microenvironment and peri-vasculature, which leads to the immaturity of SC-derived islets, reducing their ability to detect glucose fluctuations and insulin release. Here, we bioengineer the in vivo-like pancreatic niches by optimizing the combination of pancreatic tissue-specific extracellular matrix and basement membrane proteins and utilizing bioprinting-based geometrical guidance to recreate the spatial pattern of islet peripheries. The bioprinted islet-specific niche promotes coordinated interactions between islets and vasculature, supporting structural and functional features resembling native islets. Our strategy not only improves SC-derived islet functionality but also offers significant potential for advancing research on islet development, maturation, and diabetic disease modeling, with future implications for translational applications.https://doi.org/10.1038/s41467-025-56665-5 |
| spellingShingle | Myungji Kim Seungyeon Cho Dong Gyu Hwang In Kyong Shim Song Cheol Kim Jiwon Jang Jinah Jang Bioprinting of bespoke islet-specific niches to promote maturation of stem cell-derived islets Nature Communications |
| title | Bioprinting of bespoke islet-specific niches to promote maturation of stem cell-derived islets |
| title_full | Bioprinting of bespoke islet-specific niches to promote maturation of stem cell-derived islets |
| title_fullStr | Bioprinting of bespoke islet-specific niches to promote maturation of stem cell-derived islets |
| title_full_unstemmed | Bioprinting of bespoke islet-specific niches to promote maturation of stem cell-derived islets |
| title_short | Bioprinting of bespoke islet-specific niches to promote maturation of stem cell-derived islets |
| title_sort | bioprinting of bespoke islet specific niches to promote maturation of stem cell derived islets |
| url | https://doi.org/10.1038/s41467-025-56665-5 |
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